Publications by authors named "Aida Z Kebede"
Article Synopsis
- Stem rust is a significant disease affecting cultivated oats, primarily controlled in North America through host resistance from a limited number of resistance genes.
- Researchers focused on a specific resistance gene to develop high-density genetic maps and to create efficient DNA markers for selecting resistant oat varieties.
- Their findings included identifying key markers linked to the resistance gene, which were successfully converted into KASP assays that can accurately predict the presence of the resistance gene in various oat lines and cultivars.
Article Synopsis
- Crown rust, a major disease affecting oats caused by the fungus f. sp. Eriks., leads to significant yield losses and is controlled in part by a gene-for-gene interaction that oat breeders utilize.
- The study aimed to map a specific resistance gene in two independent oat populations and identify SNP markers to predict its presence in breeding material.
- Results showed the resistance gene mapped to linkage group Mrg11 with specific SNP markers, allowing for the development of PCR assays to accurately predict resistance status and improve oat breeding programs through marker-assisted selection.
SNP loci linked to the crown rust resistance gene Pc98 were identified by linkage analysis and KASP assays were developed for marker-assisted selection in breeding programs. Crown rust is among the most damaging diseases of oat and is caused by Puccinia coronata var. avenae f.
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- * Pg13 is crucial for stem rust resistance in North American oat cultivars and will aid in routine selection practices like gene pyramiding and backcrosses.
- * High-density linkage maps and genome-wide associations confirmed Pg13's location at approximately 67.7 cM on linkage group Mrg18, coinciding with specific translocation breakpoints and other resistance genes, with reliable KASP assays created for breeding use.
Molecular mapping of crown rust resistance genes is important to effectively utilize these genes and improve breeding efficiency through marker-assisted selection. is a major race-specific crown rust resistance gene initially identified in the wild hexaploid oat in the early 1970s. This gene was transferred to cultivated oat () and has been used as a differential for identification of crown rust races since 1974.
View Article and Find Full Text PDFBackground: Gibberella ear rot (GER) is one of the most economically important fungal diseases of maize in the temperate zone due to moldy grain contaminated with health threatening mycotoxins. To develop resistant genotypes and control the disease, understanding the host-pathogen interaction is essential.
Results: RNA-Seq-derived transcriptome profiles of fungal- and mock-inoculated developing kernel tissues of two maize inbred lines were used to identify differentially expressed transcripts and propose candidate genes mapping within GER resistance quantitative trait loci (QTL).
Unique and co-localized chromosomal regions affecting Gibberella ear rot disease resistance and correlated agronomic traits were identified in maize. Dissecting the mechanisms underlying resistance to Gibberella ear rot (GER) disease in maize provides insight towards more informed breeding. To this goal, we evaluated 410 recombinant inbred lines (RIL) for GER resistance over three testing years using silk channel and kernel inoculation techniques.
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