Effects of disulfiram on excitation-contraction coupling in rat soleus muscle.

The aim of this study was to analyze whether disulfiram could affect excitation-contraction coupling in rat slow-twitch ( soleus) muscle.In small bundles of intact fibers, the amplitude and the time constant of relaxation of twitch and potassium contractures were dose-dependently and reversibly reduced by disulfiram at concentrations up to 27 microM. At larger concentrations (up to 67.

Nandrolone decanoate reduces changes induced by hindlimb suspension in voltage-dependent tension of rat soleus muscle.

The effect of 8 weeks of nandrolone decanoate treatment (15 mg kg(-1)/week, 5 weeks under normal conditions followed by 3 weeks of unloading) was tested for the voltage-dependence of activation and steady-state inactivation of contraction in isolated small bundles (2-4 cells) of intact slow-twitch skeletal muscle in rats. Twenty-four male rats were divided into three groups (8 rats/group, weight matched) for 8 weeks: (1) control, (2) unloaded, and (3) unloaded-treated. Compared with age-matched control values (unloaded vs.

Nandrolone decanoate treatment affects sarcoplasmic reticulum Ca(2+) ATPase function in skinned rat slow- and fast-twitch fibres.

The effects of anabolic-androgenic steroid administration on the function of the sarcoplasmic reticulum (SR) pump were investigated in chemically skinned fibres from the extensor digitorum longus (EDL) and soleus muscles of sedentary rats. Twenty male rats were divided into two groups, one group received an intramuscular injection of nandrolone decanoate (15 mg x kg(-1)) weekly for 8 weeks, the second received similar weekly doses of vehicle (sterile peanut oil). Compared with control muscles, nandrolone decanoate treatment reduced SR Ca(2+) loading in EDL and soleus fibres by 49% and 29%, respectively.

Post-effects of nandrolone decanoate treatment on contractile responses of rat skeletal muscles.

The post-effects of nandrolone decanoate treatment (15 mg.kg(-1).week(-1)) were studied on contractile responses of isolated small bundles of intact slow-(soleus) and fast-(extensor digitorum longus, edl) twitch fibers in rat.

Methyl jasmonate-induced stimulation of sarcoplasmic reticulum Ca(2+)-ATPase affects contractile responses in rat slow-twitch skeletal muscle.

The purpose of this study was to determine whether methyl jasmonate, a stimulator of Ca(2+)-adenosine triphosphatase (ATPase) activity of the purified ATPase from fast-twitch skeletal muscle, could affect contractile responses in small bundles of rat isolated slow-twitch (soleus) fibers. In saponin-skinned fibers, sarcoplasmic reticulum (SR) Ca(2+) loading was performed in pCa 7.0 solution.