[Expression of quorum-sensing related genes during Enterococcus faecalis biofilm formation].

Objective: To investigate the relationship between the expression of the quorum-sensing related genes during Enterococcus faecalis(Ef) biofilm formation.

Methods: Ef biofilms model was established in vitro and film formation process was observed by confocal laser scanning microscope at 6, 12, 24 and 48 hours respectively.Quantification of biofilms was achieved by staining with crystal violet.

[The activity of osteoclast in peri-implant tissues in Beagle dogs].

Purpose: To observe the quantity and activity of osteoclast in peri-implant tissues dynamically.

Methods: An animal model of dental implants was established in 6 male Beagle dogs of 1-2 years old. Bone remodeling was tested at 3-, 7-,15-,30-,60- and 90-day after placement of implants.

[Expression of receptor activator of NF-κB ligand and osteoprotegerin in peri-implant tissues during unloading period].

Objective: To observe the expression of receptor activator of NF-κB ligand (RANKL) and its decoy receptor osteoprotegerin (OPG) during unloading period of dental implants.

Methods: An animal model of dental implants was established in Beagle dogs. Bone remodeling was tested at 3, 7, 15, 30, 60 and 90 days after the placement of implants.

[Clinical study of osteotome sinus floor elevation without grafting and with simultaneous implants placement].

Objective: To investigate the clinical results of osteotome sinus floor elevation (OSFE) without grafting combined with simultaneous implant placement.

Methods: A total of 65 patients underwent maxillary sinus floor elevation from alveolus without any bone grafting from January 2000 to December 2008 and 96 implants were placed in the maxillary posterior edentulous region simultaneously. Clinical and radiography examinations were performed.

[Clinical study of immediate placement and early loading of dental implants].

Objective: To evaluate the clinical results of immediate placement and early loading of dental implants.

Methods: Sixty-four dental implants were inserted into the edentulous section of 37 patients immediately after the teeth were extracted. 36 implants were inserted by one-stage operation procedure, of which 17 implants were loaded immediately in 3 edentulous jaws by temporary mandibular overdenture.