[Genetic variation of gag gene in HIV-1 subtype B infections from Henan and Shanxi provinces of China].

The 109 whole blood samples were collected from HIV-1 infected former blood donors in Henan and Shanxi. The RNA templates were extracted from plasma and used for the full gag gene amplification and sequencing. The sequences were divided into 3 groups according to sampling year.

The impacts of current antiretroviral therapy regimens on Chinese AIDS patients and their implications for HIV-1 drug resistance mutation.

This study sought to investigate the impacts of the antiretroviral (ARV) therapy regimens currently used in Chinese HIV-1-infected individuals. Seven hundred eighteen ARV-treated and treatment-naive HIV-1-infected individuals living in seven provinces were enrolled in 2005 by a multistage sampling approach according to a national cross-sectional survey program on HIV-1 drug resistance. All patients were investigated clinically, and CD4+ T cell counts and HIV-1 viral loads were measured while genotyping for drug resistance was determined by a home brew nested PCR.

Development and optimization of a method for analysis of the products from the transesterification of dimethyl carbonate and phenol.

A high-purity sample of methyl phenyl carbonate (MPC) was obtained by developing a novel reaction route followed by a series of separation and purification procedures. Identification and quantification of the MPC sample (98.32%) was performed by a gas chromatography-mass spectrometry and Karl Fisher titration.

[Detection of hantaan virus from gamasid mite and chigger mite by molecular biological methods].

Objective: To study the proliferation and location of hantaan virus (HV) in gamasid mites and chigger mites.

Methods: HV RNA in gamasid mites and chigger mites were detected by reverse transcription, polymerase chain reaction (RT- PCR) and in situ hybridization.

Results: The smallest quantity of mite from which HV RNA could be detected was 5 mites group.