Publications by authors named "Ai-hua Guo"
Objectives: Atherosclerosis and osteoporosis (OP) are common diseases in elderly individuals and may share common pathogenetic mechanisms. The aim of this study was to investigate the association between bone mineral density (BMD) and coronary artery calcium (CAC) in postmenopausal women.
Methods: In this cross-sectional study, 186 postmenopausal women 50-80 years of age were included.
Mycoplasma pneumoniae pneumonia (MPP) is a common disease in children. Qingfei Tongluo formula (QTF) has been used for the treatment of MPP clinically, but the chemical constituents and mechanism involved remain unclear. This study aimed to analyze the main chemical constituents and to explore the possible mechanism of action associated with QTF treatment of MPP.
View Article and Find Full Text PDF[Two cases of congenital nephrotic syndrome resulting from cytomegalovirus infection].
Zhonghua Er Ke Za Zhi
November 2007
Objective: To construct an cell penetrating peptide-based expression vector capable of targeted delivery of proteins into the cell nuclei and study its function of protein transduction.
Methods: The fusion protein expression vector pET14b-HC(L)NE (pET14-b-His-CPP-Linker-NLS-EGFP) incorporating cell penetrating peptide (CPP), nuclear localization signal(NLS), linker and enhanced green fluorescent protein (EGFP) was constructed based on His-tagged pET14b-HE (pET14b-His-EGFP) by site-directed mutagenesis PCR method. After identification by enzyme digestion and DNA sequencing, the recombinant plasmid was transformed into BL21(DE(3)) strain.
[Reconstruction of an intracellular transduction system based on HIV-1 TAT protein transduction domain].
Nan Fang Yi Ke Da Xue Xue Bao
May 2006
Objective: To explore the reasons for the low intracellular transduction efficiency of a previously constructed His-TAT-Flag recombinant protein and establish a more efficient transduction system.
Methods: The Flag tag of pET14b-His- Tat-Flag vector was deleted with PCR mutant kit, and enhanced green fluorescent protein (EGFP) coding sequence was inserted into the new pET14b-His-TAT recombinant vector. Enzyme digestion and DNA sequencing were performed for identification of pET14b-His-TAT-EGFP vector, which was then transformed into E.
Objective: To assess the value of helical CT in the diagnosis of liver diseases.
Methods: 59 patients with different liver diseases were examined by two-phase or multi-phase dynamic helical CT.
Results: Small hepatocellular carcinoma showed a higher density in the arterial phase, and a lower density in the portal vein phase.
Objective: To study the transcriptional regulation of inducible nitric oxide synthase (iNOS) gene by p38 mitogen-activated protein kinase (MAPK).
Methods: With human embryonic kidney (HEK) 293 cells as the target and the assistance of lipofectamine-mediated co-transfection techniques and luciferase reporter gene systems, FLAG-tagged p38 isoforms (namely FLAG-p38 alpha, FLAG-p38 beta;, FLAG-p38 gamma and FLAG-p38 phi;) in pcDNA3, pcDNA3, piNOS-Luc and pCMV-beta; were transfected into HEK 293 cells, and the relative activity of luciferase was subsequently tested.
Results: Highest luciferase activity occurred only in p38 alpha group compared with the other three isoform groups under no stimulation.
Article Synopsis
- * Methods: Various chromatography techniques were employed to separate the compounds, and extensive spectral analysis was used to identify their structures.
- * Results: Five compounds were isolated, including a new compound called penicillixanthone A, while the other four were identified from Penicillium thomii for the first time.