Three Dimensional Solution Structure of Neurotoxin CM-11 from the Ophiophagus hannah.

The Ophiophagus hannah (King Cobra) neurotoxin CM-11 is a small protein with 72 amino acid residues. Based on complete assignments of (1)H-NMR resonances and determination of secondary structures of CM-11, 349 distance and 27 dihedral angle constraints including 19 psi's and 8 Xi (1)'s were collected from NOESY and DQF-COSY, and the chemical stereospecific assignment of Beta(1)H was partially achieved. Twelve structures with lower energy were obtained via metric matrix distance geometry and refinement with simulated annealing.

Determination of the Secondary Structure of the king Cobra Neurotoxin CM-11.

The king cobra neurotoxin CM-11 is a small protein with 72 amino acid residues. After its complete assignments of (1)H-NMR resonance's were obtained using various 2D-NMR technologies, including DQF-COSY, clean-TOCSY and NOESY, the secondary structure was analysed by studying the various NOEs extracted from the NOESY spectra and the distribution of chemical shifts. The secondary structure was finally determined by MCD as follows: a triple-strand antiparallel beta sheet with I20-W26, R37-A43 and V53-S59 as its beta strands, a short alpha helix formed by W30-G35 and four turns formed by P7-K1O, C14-G17, K50-V53 and D61-N64.

Complete Assignment of (1)H-NMR Resonances of the King Cobra Neurotoxin CM-11.

The king cobra (Ophiophagus Hannah) neurotoxin CM-Il is long-chain peptide with 72 amino acid residues. Its complete assignment of (1)H-NMR resonances was obtained using various 2D-NMR technologies, including DQF-COSY, clean-TOCSY and NOESY.