[The study of dynamic acccumulation of camptothecin in Camptotheca acuminata fruit by TLCS].

Objective: To establish TLC scanning method for the determination of camptothecin in Camptotheca acuminata fruit, and analysis the dynamic accumulation of camptothecin in Camptotheca acuminata fruit to find out the best collection period.

Methods: Silica gel H-CMC-Na thin layer plate was adopted in the determination with chloroform-acetone (7 : 3) used as deeloper, Single-wavelength and linear scanning of TLC was used, and the detection wavelength was 360 nm.

Results: There was a good linear relationship for Comptothecin within the range of 0.