Effects of spraying cycocel and delaying nitrogen application on lodging resistance and yield formation of winter wheat.

We conducted a 3-year (2017-2020) field experiment in the wheat base of Jinzhong Agricultural Hi-tech Industries Demonstration Zone, aiming to determine the measures of nitrogen topdressing and the regulatory effect of cycocel in spring to increase wheat yield. Four nitrogen topdressing dates were set up under the condition of cycocel spraying and control (CK) during the rising period: 10 days (D10), 20 days (D20), 30 days (D30), and 40 days (D40) after regreening stage, to analyze the impact of different N topdressing dates on winter wheat yield and the regulation effect of cycocel on stem characteristics, lignin content and related synthetase activities. The results showed that compared to other nitrogen topdressing dates, D30 increased spike number by 1.

[Effects of Long-term Tillage on Soil Bacterial Community Structure and Physicochemical Properties of Dryland Wheat Fields in Northern China].

To explore the effects of long-term tillage on bacterial community structure in different soil layers of dryland wheat fields and its relationship with soil physicochemical properties, a long-term field experiment was conducted from 2016 to 2021 in Wenxi Experimental Demonstration Base of Shanxi Agricultural University, Shanxi Province. We studied the effects of no-tillage (NT), subsoiling-tillage (ST), and deep plowing (DP) on soil physicochemical properties; and diversity of the bacterial community; and dominant and different species of phyla and genera in different soil layers. Additionally, PICRUSt2 was used to predict the metabolic function of soil bacterial community.

[Effects of deep ploughing during the fallow period and soil moisture-based furrow sowing on water and nitrogen utilization of dryland wheat.].

To understand the effects of deep ploughing during the fallow period and soil moisture-based furrow sowing on the utilization of water and nitrogen in dryland wheat, a field experiment following split-plot design was carried out from 2016 to 2018 in Wenxi County of Shanxi Province, with deep ploughing during the fallow period and no-tillage as main plots and soil moisture-based furrow sowing and drilling sowing as sub-plots. The results showed that, compared with no-tillage treatment, deep ploughing during the fallow period significantly increased soil water storage efficiency (by 38.3%-42.

Soil physical properties response to tillage practices during summer fallow of dryland winter wheat field on the Loess Plateau.

Soil physical properties are a greatly important part of the soil and indicator of soil quality, which can directly affect soil nutrient turnover and crop yields in dryland. This study was carried out with three tillage practices during the summer fallow season since 2011, including no tillage (NT), plow tillage (PT), and subsoiling (ST) in dryland winter wheat fields of the Loess Plateau. Results showed that soil tillage during the summer fallow had a small effect on soil bulk density (ρ ) in the 0-50-cm soil profile before sowing and after harvesting of winter wheat.

[Relationships of water conservation through mulching in fallow period with wheat nitrogen transportation and crop yield in dryland].

Field experiments were carried out to study the effects of different mulching times (30 or 60 d after previous wheat harvest) and mulching methods (whole-mulching, half-mulching and no- mulching) on wheat plant N absorption and assimilation after deep plowing in fallow period on dryland. The results showed that mulching improved water storage efficiency in fallow period and soil water storage of 0-300 cm at sowing stage significantly. Mulching in fallow period, especially the whole-mulching, increased the N accumulation amount of each growth stage, N translocation amount and rate before anthesis (NABA) , and N accumulation amount after anthesis (NAAA) and grain N.

[Effects of tillage in fallow period on soil water and nitrogen absorption and translocation by wheat plant].

Field test was carried out to study the effect of tillage in fallow period on soil water before sowing and growth stages, and nitrogen (N) absorption, translocation by wheat plant. The current data showed that tillage in fallow period improved the soil water at the depth of 0-300 cm before sowing and growth stages, especially in dry years. Such tillage significantly improved N accumulation in leaf, stem and sheath (SS) at anthesis, grain N accumulation at maturity, N mobilization in SS and the contribution of mobilized N to grain N, amount of mobilized N in leaf, level of N accumulation before anthesis, N transportation from vegetative organs to grains after anthesis, and nitrogen accumulation after anthesis, which in turn enhanced the efficiency of N uptake.

Effect of subsoiling in fallow period on soil water storage and grain protein accumulation of dryland wheat and its regulatory effect by nitrogen application.

To provide a new way to increase water storage and retention of dryland wheat, a field study was conducted at Wenxi experimental site of Shanxi Agricultural University. The effect of subsoiling in fallow period on soil water storage, accumulation of proline, and formation of grain protein after anthesis were determined. Our results showed that subsoiling in fallow period could increase water storage in the 0-300 cm soil at pre-sowing stage and at anthesis stage with low or medium N application, especially for the 60-160 cm soil.

[Expression and activity determination of TNFR domain of osteoprotegerin in E.coli and corresponding antibody preparation].

Osteoprotegerin (OPG) plays an important role in the regulation of bone resorption and remodeling. The TNFR domain of OPG, which is involved in the inhibition of formation and activity of osteoclasts, was amplified by PCR and inserted into multiple cloning site of PET-28a. The recombinant plasmid was transferred into E.

Expression, purification and sublocalization of SARS-CoV nucleocapsid protein in insect cells.

The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients. To facilitate the studies on the function and structure of the N protein, this report describe the expression and purification of recombinant SARS-CoV N protein using the baculovirus expression system.