Chemical constituents from Notopterygium incisum and their anti-neuroinflammatory activity.

Phytochemical research on an extract of Notopterygium incisum yielded fifteen compounds (1-15), including four previously undescribed compounds (10-13). The structures of the unreported compounds were elucidated by spectroscopic and spectrometric data analysis such as 1D and 2D NMR, IR and HR-ESI-MS. Compounds 1-5 and 10-14 were isolated from N.

AgSbF-catalyzed C3 -Friedel-Crafts alkylation of ,-acetals with indoles for the synthesis of indole substituted pyrrolidine and piperidine derivatives.

An efficient approach to access chiral indole substituted pyrrolidine and piperidine skeletons has been developed through a AgSbF-catalyzed -Friedel-Crafts alkylation of ,-acetals 6a, 6b, 9, and 11a-11d with indoles. As a result, a series of 2,3- indole substituted pyrrolidines 8a-8x and piperidines 10a-10j were prepared in moderate to excellent yields and with excellent diastereoselectivities ( up to 99 : 1). Moreover, several 2,5- indole substituted pyrrolidine derivatives 12a-12k were synthesized according to this strategy with moderate to good yields and diastereoselectivities ( up to 99 : 1).

Zn(OTf)-catalyzed hydroamination of ynamides with aromatic amines.

The Zn(OTf)-catalyzed hydroamination of ynamides 2a-2l with aromatic amines 1a-1r was developed. This protocol features broad substrate scope of aromatic amines, good functional group tolerance for ynamides, and excellent regioselectivities. As a result, a variety of substituted amidine compounds 3aa-3oa, 3ab-3al and 3pa-3rk were prepared in moderate to excellent yields and with high regioselectivities.

Another Look at Pyrroloiminoquinone Alkaloids-Perspectives on Their Therapeutic Potential from Known Structures and Semisynthetic Analogues.

This study began with the goal of identifying constituents from extracts that showed selectivity in our primary cytotoxicity screen against the PANC-1 tumor cell line. During the course of this project, which focused on six . samples collected from various regions of the Indo-Pacific, known compounds were obtained consisting of nine makaluvamine and three damirone analogues.

[ORMDL3 polymorphisms and their relationship with OPN and TGF-β1 levels in children with asthma in Hunan, China: an analysis of 98 cases].

Objective: To investigate ORMDL3 polymorphisms in children with asthma in Hunan, China, and to determine the relationship between ORMDL3 polymorphisms and serum osteopontin (OPN) and transforming growth factor-β1 (TGF-β1) levels.

Methods: Peripheral blood samples were collected in children with asthma (n=98; astma group) or without asthma (n=30; control group) from Hunan, China. The asthma group was subdivided into atopic (n=62) and non-atopic (n=36) subgroups.

[Effect of budesonide aerosol treatment on expression of glucocorticoid receptor and nuclear factor-κB in asthmatic mice].

Objective: To study the effect of budesonide aerosol inhalation on the expression of glucocorticoid receptor (GR) and nuclear factor (NF)-κB in asthmatic mice.

Methods: Twenty-four healthy male BALB/c mice aged 6 to 8 weeks were randomly divided into three groups (n=8 each): normal saline (control group), asthma model (asthma group) and budesonide-treated asthma (BUD group). Asthma was induced by intraperitoneal injection of ovalbumin (OVA) and aluminium hydroxide suspension and aerosol inhalation of OVA solution.

[Extraction and purification of isocorydine from Dicranostigma leptopodum].

Objective: To optimize the conditions of extraction and purification of isocorydine from Dicranostigma leptopodum.

Methods: Extraction conditions of isocorydine were selected on the basis of orthogonal experimental design, the static adsorption/desorption experiments were used to evaluate the optimum resin.

Results: The optimum extraction parameters were as follows: the ratio of raw materials to solvent 1:15 (g/mL), extraction solvent 1% vitriol, the extraction three times and 1 h each time.

[Mycoplasma pneumoniae infection and drug resistance in children: an analysis of 1026 cases].

Objective: To investigate the Mycoplasma pneumoniae (MP) infection and drug resistance in children with respiratory tract infection and to provide a rational basis for the clinical diagnosis and treatment of MP infection.

Methods: Throat swabs were collected from 3529 children with respiratory tract infection, who visited the pediatric outpatient department or received treatment in the pediatric ward of our hospital from September 2010 to September 2011. The swabs were cultured to detect MP.

[Study on the chemical constituents from Clematis brevicaudata].

Objective: To study the chemical constituents from Clematis brevicaudata.

Methods: The compounds were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR).

Results: Eight compounds were isolated and identified as: palmitic acid (1), 1-docosanol (2), pentacosanoic acid-2', 3'-dihydroxypropyl ester (3), beta-sitosterol (4), daucosterol (5), a mixture of the trans-p-coumarate of the n-alkanols (6), 3,4-dihydroxy-trans coumatate ethyl ester (7), syringaresinol-O-D-glucopyranoside (8).

[Chemical constituents from Pyrethrum tatsienense].

Objective: To isolate and identify the chemical constituents from the whole plant of Pyrethrum tatsienense, which has been used as traditonal herb medicine in the treatment of some diseases.

Methods: The compounds were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR).

Results: Six compounds were isolated and identified as: linoeic acid, pinoresinol, (+)-pinoresinol-4'-O-beta-D- glucopyranoside, betulabuside A, dihydrosyringin.

Isolation, identification, and comparison of four isolates of avian paramyxovirus serotype 2 in China.

Four Yucaipa-like viruses of avian paramyxovirus serotype 2 (APMV-2) were isolated in China from the imported Gouldian Finch (Chloebia gouldiae) and broilers in 1998-2002, and were named F4, F6, F8, and NK, respectively. Examined under electron microscope, the isolates were found to be round in shape and varying in size. The results of the hemagglutination inhibition test and indirect enzyme-linked immunosorbent assay (using monoclonal antibodies) showed some differences between the isolates and the reference strain Yucaipa.

Triterpenoids from pyrethrum tatsienense.

Two novel and eight known triterpenoids were isolated from alcoholic extract of the whole plant of Pyrethrum tatsienense. The structures of new compounds were elucidated as olean-12-en-3beta,11alpha,16beta-triol-3-O-palmitate (1), ursa-12-en-3beta,11beta,16beta-triol-3-O-palmitate (2) by spectroscopic methods including intensive 2D NMR techniques (H1-H1 COSY, gHMQC, gHMBC) and FAB-MS, and the known compounds were identified on the basis of comparing their NMR data with those of corresponding compounds in the literature.

[Studies on the alkaloids from the herb of Corydalis adunca].

Objective: To study the alkaloid constituents of Corydalis adunca.

Method: The constituents were isolated on silica gel column and their structures were elucidated by IR, NMR, MS data.

Result: Eight alkaloid compounds were isolated from alcohol extracts of the herb of C.