Simple dual-readout immunosensor based on phosphate-triggered and potassium permanganate for visual detection of fenitrothion.

Multicolor-based visual immunosensor is a promising tool for rapid analysis without the use of bulky instruments. Herein, an anti-fenitrothion nanobody-alkaline phosphatase fusion protein (VHHjd8-ALP) was employed to develop a multicolor visual immunosensor (MVIS) and a ratiometric fluorescence MVIS (RFMVIS, respectively). After one-step competitive immunoassay, the VHHjd8-ALP bound to microplate catalyzed phenyl phosphate disodium salt (ArP) into phenol.

The Analysis of Differentially Expressed circRNAs Under the Antiproliferative Effect From 5-Fluorouracil on Osteosarcoma Cells.

Background: 5-fluorouracil (5-FU) is a widely used drug for cancer treatment, but its effect and underlying mechanisms on osteosarcoma (OS) cells remain unclear.

Methods: U2OS and MG63 cells were treated with 0, 50, 100, and 500 μM 5-FU. MTS and flow cytometry were used to examine the effect of 5-FU on cell viability and apoptosis, respectively.

Modeling Citrus Huanglongbing transmission within an orchard and its optimal control.

Citrus Huanglongbing (HLB) is the most devastating citrus disease worldwide. In this paper, a deterministic dynamical model is proposed to explore the transmission dynamics of HLB between citrus tree and Asian citrus psyllid (ACP). Using the theory of dynamical system, the dynamics of the model are rigorously analyzed.

Silencing of the Chitin Synthase Gene Is Lethal to the Asian Citrus Psyllid, .

Chitin synthase is a critical enzyme that catalyzes -acetylglucosamine to form chitin, which plays an important role in the growth and development of insects. In this study, we identified a chitin synthase gene () with a complete open reading frame (ORF) of 3180 bp from the genome database of , encoding a protein of 1059 amino acid residues with the appropriate signature motifs (EDR and QRRRW). Reverse transcription-quantitative PCR (RT-qPCR) analysis suggested that () was expressed throughout all developmental stages and all tissues.

The stimulating effects of polyphenol and protein fractions from jelly fig (Ficus awkeotsang Makino) achenes against proliferation of leukemia cells.

This study aimed to investigate the direct and immune-stimulated antiproliferative activities of jelly fig achenes fractions including pectinesterase inhibitors, crude polyphenols extract, and purified polyphenols extract (PP). Beside the measurement of cell viability of U937, the quantity of cytokines in conditioned medium and morphologic changes in leukemia were observed. After surveying all fractions in jelly fig, the obtained fractions of polyphenol exhibited the highest stimulating effects and directly cytotoxic effects against leukemia with the lowest effect found in protein fractions.

Entropic uncertainty relations for Markovian and non-Markovian processes under a structured bosonic reservoir.

The uncertainty relation is a fundamental limit in quantum mechanics and is of great importance to quantum information processing as it relates to quantum precision measurement. Due to interactions with the surrounding environment, a quantum system will unavoidably suffer from decoherence. Here, we investigate the dynamic behaviors of the entropic uncertainty relation of an atom-cavity interacting system under a bosonic reservoir during the crossover between Markovian and non-Markovian regimes.

Involvement of inflammasome activation in lipopolysaccharide-induced mice depressive-like behaviors.

Aims: The NLRP3 inflammasome is a cytoplasmic multiprotein complex of the innate immune system that regulates the cleavage of interleukin-1β and interleukin-18 precursors. It can detect a wide range of danger signals and trigger a series of immune-inflammatory reactions. There were plenty of studies indicating that activation of the immune system played pivotal roles in depression.

[Literature analysis of schistosomiasis control in Poyang Lake region].

Objective: To understand the methods and effects of schistosomiasis control in Poyang Lake region of Jiangxi Province.

Methods: The related literature in Chinese Biomedical Database and PubMed Database was searched and selected according to the inclusion and exclusion criteria. The methods of bibliometrics and Meta analysis were performed to analyze the literature.