Downregulation of lncRNA IGF2-AS-encoded peptide induces trophoblast - cycle arrest.

Research Question: lncRNA IGF2-AS may be related to early pregnancy loss. Does lncRNA IGF2-AS affect trophoblast cell growth? The aim of the present study was to verify that lncRNA IGF2-AS encodes a polypeptide, IGF2-AS-168aa, and to study its role in the pathogenesis of trophoblasts.

Design: A small interfering RNA targeted to the IGF2-AS gene (si-IGF2-AS) was designed and transfected into JEG-3 and JAR cells for in-vitro gene silencing.

Aberrant methylation of IGF2-AS promoter in early pregnancy loss.

Objective: The present study aimed to evaluate insulin-like growth factor 2 antisense (IGF2-AS) in the villi of human embryos and compared its expression between normal pregnancy and early pregnancy loss (EPL).

Materials And Methods: The present study conducted a microarray analysis to identify the expression profiles of lncRNAs in villi from EPL and normal controls (controls, n = 10 and EPL patients, n = 10). Embryonic villi were collected from women who underwent artificial abortion.

[Phytoplankton community structure and assessment of water quality in the middle and lower reaches of Fenhe River].

To understand the distribution of phytoplankton and the water quality in the middle and lower reaches of Fenhe River, 18 sampling sites were selected for specimen collection, species identification and data analysis. The results showed that: (1) There were 298 species of phytoplankton under the membership of 8 divisions and 96 genera, among which, Bacillariophyta was the dominant division, with a total of 127 species of 27 genera, followed by Chlorophyta, with 104 species of 41 genera, and Cyanophyta, with 45 species of 20 genera. Only 22 species of 8 genera belonged to Euglenophyta, Cryptophyta, Pyrrophyta, Chrysophyta and Xanthophyta.