Publications by authors named "Ai Ishiguro"
Granulosa cells (GCs) contribute to oocyte development. The present study addressed the effect of cryopreservation on the ability of GCs to support oocyte development. GCs were collected from antral follicles.
View Article and Find Full Text PDFPurpose: This study aimed to examine the relationship between granulosa cells (GCs), number of follicles, and the ability of follicular fluid to support in vitro growth of oocytes.
Methods: The culture medium was supplemented with follicular fluid (FF) collected from GC-rich ovaries and GC-poor ovaries, and its effect on in vitro growth and quality of oocytes derived from early antral follicles (EAFs) was assessed.
Results: GC-rich FF treatment enhanced oocyte growth and augmented changes in the chromatin configuration and lipid content of oocytes when compared to oocytes treated with GC-poor FF.
This study compared the effects of different volumes of culture medium for the in vitro growth of oocytes derived from porcine early antral follicles (EAFs). Oocyte granulosa cell complexes (OGCs) were collected from EAFs (0.5-0.
View Article and Find Full Text PDFPurpose: Improvement of in vitro oocyte growth by addition of granulosa cells derived from differential developmental stages of follicles.
Methods: Granulosa cells (GCs) collected from either early antral follicles (EAFs) or antral follicles (AFs) were added to oocyte-granulosa cell complexes (OGCs) derived from EAFs, and the in vitro growth of the oocytes was evaluated.
Results: Granulosa cells were incorporated into OGCs to form new OGCs within 2 days of culture.