Publications by authors named "Ahmet Adiguzel"

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  • The study focuses on using waste frying oil as a carbon source to produce cost-effective rhamnolipids, overcoming common production barriers.
  • Optimal conditions for rhamnolipid production were identified, resulting in a yield of 2.97 g/l at specific temperature, pH, and incubation time.
  • The research indicates that using Halopseudomonas sabulinigri OZK5 for biosurfactant production is a promising biotechnological approach due to its eco-friendliness and efficiency.
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  • The study aimed to adapt the Vestibular Migraine Patient Assessment Tool and Handicap Inventory (VM-PATHI) for use in Turkey due to a lack of diagnostic tools for vestibular migraine.
  • Researchers conducted pilot studies and various analyses (EFA, CFA) to validate the tool's language, structure, and reliability.
  • Results showed strong validity and reliability of the VM-PATHI, making it an effective tool for assessing vestibular migraine in the Turkish population.
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The production of the extracellular polysaccharide from the thermophilic bacterium was carried out in the study. The polysaccharide was isolated and characterized by means of GC-MS, FT-IR, DSC, and XRD analyses. The rheological, foaming, and emulsifying properties of the polysaccharide were determined.

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  • The study introduces a sustainable method for creating copper nanoparticles (Cu NPs) using enzymes from a thermophilic bacterium, offering a greener alternative to traditional synthesis methods.
  • The production process utilized optimized conditions involving lignin peroxidase enzyme derived from peanut waste, emphasizing waste valorization and environmental benefits.
  • Characterization of the Cu NPs confirmed their stability and shape, and they demonstrated significant antibacterial activity against various pathogens, particularly showing effectiveness against Streptococcus pyogenes, which suggests potential uses in medicine and other fields.
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  • Meat provides an ideal environment for foodborne pathogens like Listeria monocytogenes, Salmonella enterica, and Escherichia coli O157:H7, which can lead to serious health issues.
  • A study surveyed 100 minced beef samples from different butchers in Erzurum province and used Real-Time PCR for pathogen detection, finding Listeria in 45 samples, E. coli in 30, and Salmonella in 29.
  • The Real-Time PCR method showed higher efficiency and reliability compared to traditional techniques, allowing for quicker, more cost-effective, and less labor-intensive detection of these harmful bacteria.
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Background: It is reported that antiepileptic drugs have an effect on balance functions. The aim of the study was to evaluate and compare the effects of valproic acid and levetiracetam monotherapy on balance functions in patients with generalized epilepsy using objective test methods.

Methods: The study included 43 generalized epilepsy patients aged 18-60 years, including 20 patients receiving valproic acid monotherapy, 23 patients receiving levetiracetam monotherapy, and 25 healthy individuals as controls, in the Neurology Clinic of a university hospital in eastern Turkey.

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Dyslipidemia is a major atherogenic risk factor for ischemic stroke. Stroke patients tend to have high levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) and low levels of high-density lipoprotein cholesterol (HDL-C). Therefore, it is noteworthy that there has been an increase in ischemic stroke cases in young and elderly individuals in recent years.

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  • The Bacillaceae family, especially the genus Anoxybacillus, is gaining attention for its potential in biotechnological processes due to its ability to produce thermostable enzymes and spore-forming capabilities.
  • Anoxybacillus, a relatively new genus since 2000, shows promise in applications like waste management and bioremediation, helping to break down toxic substances in various industrial processes.
  • Recent research highlights the diverse Anoxybacillus strains from different environments and their various biotechnological applications, including enzyme production and the creation of bioactive exopolysaccharides with antimicrobial and anticancer properties.
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Peptones are one of the most expensive components of microbial culture media. The present study was conducted to test the usability of low-cost sheep wool peptone (SWP) as an organic nitrogen source in the production of six industrially important enzymes (lipase, amylase, tannase, pectinase, cellulase and invertase). SWP was prepared by alkaline hydrolysis and acid neutralization.

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Millions of tons of agricultural waste are produced globally every year. A practical solution to this global problem is to convert this waste into value-added products. In this study, endoglucanase enzyme production was carried out by using waste melon peels as a carbon source.

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  • Three-phase partitioning (TPP) is an effective method for purifying laccases, achieving a 4.9-fold purification and 110% recovery from a highly active A2 strain isolated from Agri-Diyadin hot spring.
  • The A2 isolate was identified as Enterococcus faecium, showing impressive laccase characteristics, including a molecular mass of 50.11 kDa, optimal activity at pH 6.0 and 80 °C, and outstanding stability across a variety of environments.
  • The enzyme demonstrated remarkable thermostability, maintained activity in harsh conditions with metal ions, surfactants, and organic solvents, and exhibited precise kinetic values when tested with the ABTS substrate.
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Objective: The clinical presentation of coronavirus disease 2019 may be more severe in individuals with diagnoses such as neurodegenerative diseases and cerebrovascular disease, which may occur at an advanced age, among the underlying chronic neurological disorders. In this study, we analyzed the incidence of underlying neurological disorders, the clinical process, the effects on prognosis, duration of hospitalization, and clinical parameters such as mortality and the incidence of neurological manifestations that occurred in the study group after being infected and their relationship with the prognosis in patients hospitalized due to coronavirus disease 2019.

Materials And Methods: This is a retrospective and single-centered study.

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This study was performed to elucidate the effects of two fungal quorum sensing molecules (tyrosol and farnesol) on carotenoid synthesis in the yeast Rhodotorula glutinis and prodigioin synthesis in the bacterium Serratia marcencens. Farnesol or tyrosol was directly added to the flask cultures at the beginning (immediately after inoculation with the preculture) of day 1 or the beginning (49th h) of day 3. The results demonstrated that tyrosol supplementation increased the synthesis of carotenoids but farnesol supplementation increased the synthesis of prodigiosin.

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Background: Complete blood count derived indexes such as lymphocyte-to-neutrophil ratio (NLR) may help in predicting pneumonia and prognosis in acute stroke. However, the optimal time point for using these biomarkers is not known.

Methods: In 205 consecutive severe (NIHSS>10) acute ischemic stroke patients, daily leukocyte, lymphocyte, neutrophil, monocyte, platelet, albumin, fibrinogen, hematocrit, NLR, PLR (Platelet-to-lymphocyte-ratio), LMR (Lymphocyte-to-monocyte-ratio), and SII (systemic-immune-inflammation-index) were determined.

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  • The study focuses on extracting chitin from shrimp shell powder using a specific bacteria, Paenibacillus jamilae, and making chitosan from it.
  • The bacteria did not have chitinase but showed strong protease activity, with optimal conditions for deproteinization being pH 7.0, a shell concentration of 60 g/L, and an incubation period of 4 days.
  • The resulting chitosan had a higher deacetylation degree and better antimicrobial and antioxidant properties compared to commercial chitosan, marking a significant finding in biological chitin extraction.
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  • The study examined how different temperatures of NaOCl irrigation affect postoperative pain and bacteria levels in root canal treatments.
  • Results showed that all temperature groups significantly reduced bacterial count, but the group with cold NaOCl (2˚C) experienced less postoperative pain compared to the warmer NaOCl (45˚C).
  • Overall, while antibacterial effectiveness was similar across temperatures, cold NaOCl was found to be more beneficial for minimizing pain after treatment.
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Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries. This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646). EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities.

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A novel Gram-stain-positive, rod-shaped, endospore-forming, motile, aerobic bacterium, designated as P2, was isolated from a hot spring water sample collected from Ilica-Erzurum, Turkey. Phylogenetic analyses based on 16S rRNA gene sequence comparisons affiliated strain P2 with the genus , and the strain showed the highest sequence identity to NBRC 15712 (96.7 %).

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  • Strain P1, a Gram-positive, endospore-forming bacterium, was isolated from Pasinler Hot Spring and characterized to determine its taxonomic classification.
  • It shares high genetic similarity with known strains R-6488 (97.6%) and MO-04 (97.2%), and forms a unique group in phylogenetic analyses based on 16S rRNA gene sequences.
  • Strain P1 thrives at 55°C and 2% NaCl, contains specific cell-wall components, and is proposed as a new species within the genus, named sp. nov., with type strain P1.
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Background: In patients with normal pressure hydrocephalus (NPH), lumbar puncture (LP) is an effective method for assessing both the diagnosis as well as the possible efficacy of ventriculoperitoneal shunting based on symptom improvement. However, it should be considered that there exists a low risk of complications and that these risks may result in morbidity and mortality. We present a patient who developed hematoma in the basal ganglia following LP.

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Non-sterile culture technique is currently used in some microbial processes. However, there is no study on the use of this technique in the production of microbial lipases and hydrolysis of waste frying oils. This study was conducted to hydrolyse waste frying oils and produce lipase under non-sterile culture conditions using locally isolated cold-adapted bacteria.

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A novel extracellular xylanase was purified and characterized from Pediococcus acidilactici GC25 (GenBank number: MF289522). The purification was 4.6-fold with a yield of 43.

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In this study, isolation, conventional and molecular characterizations of ten thermophilic bacteria from Rize/Ayder were carried out. Xylanase from Geobacillus galactosidasius BS61 (GenBank number: KX447660) was purified by acetone precipitation, Diethylaminoethyl-cellulose and Sephadex G-100 chromatographies. The xylanase of G.

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  • An extracellular thermostable alkaline serine protease enzyme was isolated from Aeribacillus pallidus C10, achieving a purification enhancement of 4.85 to 17.32 times with yields between 19.56% and 26.9%.
  • The enzyme has a molecular weight of approximately 38.35 kDa and shows peak activity at pH 9 and 60°C, remaining stable within a pH range of 7.0-10.0 and retaining over 80% activity between 20-80°C.
  • It also maintains significant activity in the presence of organic solvents and commercial detergents, with activity boosted by the presence of 5% SDS; kinetic parameters include K and V values of 0
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In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.

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