Rapid and Ultrasensitive Colorimetric Biosensors for Onsite Detection of O157:H7 in Fluids.

This study presents a breakthrough in the field of onsite bacterial detection, offering an innovative, rapid, and ultrasensitive colorimetric biosensor for the detection of () O157:H7, using chemically modified melamine foam (MF). Different from conventional platforms, such as 96-well plates and fiber-based membranes, the modified MF features a macroporous reticulated three-dimensional (3D) framework structure, allowing fast and free movement of large biomolecules and bacteria cells through the MF structure in every direction and ensuring good accessibility of entire active binding sites of the framework structure with the target bacteria, which significantly increased sensitive and volume-responsive detection of whole-cell bacteria. The biosensing platform requires less than 1.

A novel scalable polycationic melamine sponge-based filtration matrix for continuous ultrafast adsorption of anionic pollutants.

Effective capturing of anionic pollutants from wastewater under industrial operating conditions, which requires high processing flux and fast adsorption rate remains a challenge. Here, a commercially available melamine sponge (MS) with reticulated 3D macroporous structures was covalently modified with positively charged moieties using a single step functionalization under mild conditions. The developed novel polycationic melamine sponge (MS) was formed by a nucleophilic addition reaction between glycidyltrimethylammonium chloride (GMTA) and MS, followed by a self-propagation of GMTA.

A label-free electrochemical immunosensor based on decorated cellulose nanofibrous membrane for point-of-care diagnosis of amanitin poisoning human urine.

α-Amanitin (AMN) is one of the deadliest toxins from mushrooms, present in the deadly mushroom species . It is a bicyclic octapeptide and represents up to 40% of the amatoxins in mushrooms, damaging the liver and kidneys. Current methods of detecting amatoxins are time-consuming and require the use of expensive equipment.

Cationic microcrystalline cellulose - Montmorillonite composite aerogel for preconcentration of inorganic anions from dairy wastewater.

Development of efficient adsorbents to inorganic anions as a solid phase extraction (SPE) material is highly desirable for chromatographic analysis and pollution control. In this work we developed a new hybrid cationic microcrystalline cellulose aerogel composite. Cationic cetylpyridinium imbedded montmorillonite (CPC-MT) was uniformly entrapped in microcrystalline cellulose (MCC) to enhance anionic adsorption efficiency and mechanical stability.

Unique "posture" of rose Bengal for fabricating personal protective equipment with enhanced daylight-induced biocidal efficiency.

The aggregation-caused self-quenching of photosensitizers (PS), especially on a solid substrate, has highly limited their photo-induced biocidal efficiency in practical applications. Here, we designed a unique "posture" of rose Bengal (RB) on cotton-based super-adsorptive fibrous equipment, with RB being separately captured in the mesopores of porous organic polymers (POPs). The resultant daylight-induced biocidal cotton fabric with enhanced efficiency was named as DBwEE-Cotton.

Fabrication of polydopamine-based NIR-light responsive imprinted nanofibrous membrane for effective lysozyme extraction and controlled release from chicken egg white.

The development of highly efficient performance matrix for protein adsorption and scalable throughput adsorbent is highly desired, especially in pharmaceuticals and food industries. In this work, we present a simple methodology to prepare a nanofibrous membrane based surface molecular imprinted matrix (MIP) for selective separation of lysozyme. The MIP was developed by coating carboxylated poly (vinyl alcohol-co-ethylene) nanofibrous mat (EVOH-CCA NFM) with a near infrared (NIR)-light responsive polydopamine (PDA) layer.

Effective tetracycline removal from liquid streams of dairy manure via hierarchical poly (vinyl alcohol-co-ethylene)/polyaniline metal complex nanofibrous membranes.

Antibiotic residues from animal wastes enter underground and surface water streams, posing high risks to public health. Novel technologies capable of removing the residues from the matrix of concern such as animal waste should be developed. This research investigates the development of nanofiber absorbent for removing tetracycline (TC) antibiotic residues from liquid streams of dairy manure produced in a typical dairy farm.

Rapid removal of nitrate from liquid dairy manure by cationic poly (vinyl alcohol-co-ethylene) nanofiber membrane.

Elevated levels of nitrate in surface water is a leading concern, which impacts human and animal health, and controlling it requires improved and sustainable methods capable of removing nitrate anions from source waste water to reduce nitrate anions transport to environment. In liquid dairy manure, nitrogen content can vary from 200 to 600 ppm and the transport of manure nitrogen into ambient water through hydrologic processes has a potential to exceed the maximum contaminant level limit (10 ppm) of nitrate nitrogen for regulated public water systems. Dairy manure is considered as a reservoir of nitrate.

Daylight-Induced Antibacterial and Antiviral Cotton Cloth for Offensive Personal Protection.

Cotton fabrics with durable and reusable daylight-induced antibacterial/antiviral functions were developed by using a novel fabrication process, which employs strong electrostatic interaction between cationic cotton fibers and anionic photosensitizers. The cationic cotton contains polycationic short chains produced by a self-propagation of 2-diehtylaminoehtyl chloride (DEAE-Cl) on the surface of cotton fibers. Then, the fabric (i.

Daylight-Induced Antibacterial and Antiviral Nanofibrous Membranes Containing Vitamin K Derivatives for Personal Protective Equipment.

During the development of antibacterial and antiviral materials for personal protective equipment (PPE), daylight active functional polymeric materials containing vitamin K compounds (VKs) and impacts of polymer structures to the functions were investigated. As examples, hydrophobic polyacrylonitrile (PAN) and hydrophilic poly(vinyl alcohol--ethylene) (PVA--PE) polymers were directly blended with three VK compounds and electrospun into VK-containing nanofibrous membranes (VNFMs). The prepared VNFMs exhibited robust photoactivity in generating reactive oxygen species (ROS) under both daylight (D65, 300-800 nm) and ultraviolet A (UVA, 365 nm) irradiation, resulting in high antimicrobial and antiviral efficiency (>99.

A signal-on electrochemical aptasensor based on silanized cellulose nanofibers for rapid point-of-use detection of ochratoxin A.

An innovative ultrasensitive electrochemical aptamer-based sensor was developed for ochratoxin A (OTA) detection in cold brew coffee through revolutionary combination of nanofibers, electrochemical method, and aptamer technologies. The assembly of the aptasensor was based on the activation of silanized cellulose nanofibrous membranes as a supporting matrix for methylene blue (MB) redox probe-labeled aptamer tethering. Cellulose nanofibrous membranes were regenerated by deacetylating electrospun cellulose acetate nanofibrous membranes with deacetylation efficacy of 97%, followed by silanization of the nanofiber surfaces by using (3-aminopropyl)triethoxysilane (APTES).

Functionalized nanofibrous nylon 6 membranes for efficient reusable and selective separation of laccase enzyme.

The development of biomolecules adsorbent with superb efficiency and large processing throughput is of great importance for separation and reusing of enzymes in its free form in various vital industries. Herein, laccase enzyme adsorbent was prepared by carboxylation of nanofibrous nylon 6 membranes (Ny NFM) to form complex with copper metal ions [Ny-CCA-Cu(II)] with high selectivity for laccase adsorption with adsorption capacity 220 mg.g within 4 h.

An Innovative Nanobody-Based Electrochemical Immunosensor Using Decorated Nylon Nanofibers for Point-of-Care Monitoring of Human Exposure to Pyrethroid Insecticides.

A novel ultrasensitive nanobody-based electrochemical immunoassay was prepared for assessing human exposure to pyrethroid insecticides. 3-Phenoxybenzoic acid (3-PBA) is a common human urinary metabolite for numerous pyrethroids, which broadly served as a biomarker for following the human exposure to this pesticide group. The 3-PBA detection was via a direct competition for binding to alkaline phosphatase-embedded nanobodies between free 3-PBA and a 3-PBA-bovine serum albumin conjugate covalently immobilized onto citric acid-decorated nylon nanofibers, which were incorporated on a screen-printed electrode (SPE).

Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk.

An ultrasensitive label-free amperometric immunosensor for the detection of chloramphenicol (CAP) residues in milk has been developed by using a screen-printed carbon electrode laminated with a layer of poly (vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membrane that is covalently immobilized with a CAP antibody (anti-CAP). The performance of the PVA-co-PE nanofiber membrane (PVA-co-PE NFM) on the electrode was compared with a PVA-co-PE casted membrane (PVA-co-PE CM), necessary fabrication steps and performance of the sensors were investigated by electrochemical impedance spectroscopy (EIS). The application of the PVA-co-PE NFM decreased the electron-transfer-resistance by about 4 times compared with a conventional PVA-co-PE casted membrane.

Reusable anionic sulfonate functionalized nanofibrous membranes for cellulase enzyme adsorption and separation.

Poly (vinyl alcohol-co-ethylene) nanofibrous membranes (PVA-co-PE NFM) were successfully modified by sodium-3-sulfobenzoate to become negatively charged with sulfonate groups, and the sulfonated (PVA-co-PE) nanofiber membrane SS (PVA-co-PE NFM) was used in non-covalent adsorption of cellulases via electrostatic attraction. The modified NFM showed excellent adsorption to the enzyme molecules due to the incorporated static charge interaction with the fibers, high open-porosity and ultrahigh surface areas of the nanofibers. Such unique morphology and chemical structures lead to the adsorption capacity of 130 mg g and reusability for 5 cycles without significant change in catalytic functions.