Publications by authors named "Ahmed Wahby"
The advancement of sample preparation techniques is essential for the field of cell-based therapeutics. To obtain cells suited for clinical applications, the entire process starting from acquiring donor tissue biopsy, all through cell transplantation into the recipient, should occur in an integrated, safe, and efficient system. The current laboratory approach for solid tissue-to-cell isolation is invasive and involves multiple incoherent manual procedures running in an open operator-dependent system.
View Article and Find Full Text PDFArticle Synopsis
- - The study examines pertussis-specific antibodies in healthy Egyptian children aged 1 to 18 to understand their immunization status and the relationship between antibody levels and avidity in fighting the disease.
- - Findings indicate that younger children (6-8 years) show increased avidity, reaching a plateau in older teens, while antibody titers are highest in toddlers and young school children due to vaccination effects.
- - The research highlights that while antibody levels decrease with age, avidity increases independently, suggesting the need for avidity measurements alongside traditional tests to assess immunity and potentially guide booster vaccination strategies in Egypt.
Article Synopsis
- Researchers have identified and characterized five new snake venom metalloproteinases (SVMPs) from the highly dangerous viper species Echis pyramidum pyramidum, which vary in size and exhibit different biochemical properties.
- Three of these SVMPs (EpyB2, EpyB4, and EpyB5) exhibit hemorrhagic activity and show a strong preference for breaking down certain proteins like fibronectin and collagen, while two others (EpyB3 and EpyB6) do not cause bleeding.
- The SVMPs are stable under various temperature and pH conditions, can be inhibited by certain metal ions and metalloproteinase inhibitors, and could potentially be harnessed for medical applications related
The avidity to the corresponding antigens is often higher than to the cross-reactive antigens. This was demonstrated with the highly cross-reactive elapid Egyptian snake venoms Naja haje (Nh), Naja nigricollis (Nn) and Walterinnesia aegyptia (Wa), and used for the differentiation among the three species in a simple ELISA-based assay. A three-step immuno-affinity protocol was followed and the titer and avidity of the different antibody (Ab) preparations were assessed and evaluated.
View Article and Find Full Text PDFThis study reported the purification and characterization of a cytotoxic, neurotoxin-like protein derived from the venom of the Egyptian cobra Naja haje haje, Elapidae family, and explored their mechanistic role in the cell death. The protein purification was performed through ion-exchange chromatography and gel-filtration chromatography and was characterized by SDS-PAGE, amino acid sequencing, and mass spectrum analysis. The antitumor activity of Naja haje venom (NHV) and its fractions (NHVI, NHV-Ia, NHV-Ib, NHV-Ic, NHV-II, NHV-III, and NHV-IV) were tested against different human cancer cell lines.
View Article and Find Full Text PDF