Mining of latent feruloyl esterase resources in rumen and insight into dual-functional feruloyl esterase-xylanase from Pecoramyces ruminantium F1.

Feruloyl esterase (FAE) has been extensively studied for its crucial auxiliary effect in the biodegradation of lignocellulose. In this study, a FAE database including 15,293 amino acid sequences was established to gain a better understanding of rumen FAEs through multi-omics analysis. The higher expression level of rumen fungal FAEs over bacterial FAEs suggests that rumen fungi may have more important role in the lignocellulose degradation.

Evaluation of the use of different intraoral scanners for auricular prosthetic reconstruction.

Statement Of Problem: The use of intraoral optical scanners has been introduced for direct digital data acquisition in maxillofacial prosthetics, but their clinical accuracy has not been evaluated sufficiently.

Purpose: The purpose of this observational study was to evaluate the use of different intraoral scanners for extraoral maxillofacial digitization of an ear and to assess visually the clinical outcome of the generated 3-dimensional ear casts.

Material And Methods: A selected intact ear was scanned by using 3 different intraoral optical scanners: CEREC Primescan (Dentsply Sirona), Medit i700 (Medit Corp) and Panda P2 (Pengtum Technologies).

Effect of aging on dimensional accuracy and color stability of CAD-CAM milled and 3D-printed denture base resins: a comparative in-vitro study.

Background: There is a lack of studies comparing the dimensional accuracy and color stability of denture base resins made using computer-aided design and computer-aided manufacturing (CAD-CAM) milling, 3-dimensional (3D) printing, and conventional denture processing techniques. This makes it challenging to determine the best method to fabricate complete dentures. The objective of this in vitro investigation was to assess and contrast the color stability and dimensional accuracy of denture base resins that were 3D printed and CAD-CAM milled, both before and after aging by thermocycling using digital surface matching technology and a benchtop laser scanner without using a spray, to optimize adaptation of the denture base and cast to minimize any imperfections and to evaluate the impact of the denture cleansing solution on the stability of color.

Effect of using scan body accessories and inter-implant distances on the accuracy of complete arch implant digital impressions: An in vitro study.

Purpose: To introduce a novel design for scan body accessory parts that are reusable, easy to attach and detach without permanent change of the scan body, and can be used with different inter-implant distances to enhance the accuracy of complete arch implant scans.

Materials And Methods: A maxillary polymethylmethacrylate (PMMA) model with a soft tissue replica was fabricated with four implant analogs located at tooth positions 17, 13, 22, and 27 with 18, 25, and 30 mm inter-implant distances. Four scan bodies (SBs) were attached to the implants.

A comparative study to evaluate microstrain of low-profile attachment associated with and without bar connection in implant assisted mandibular overdenture (in vitro study).

Background: The aim of this study was to compare the microstrain transmitted to peri-implant tissues of implant-assisted mandibular overdentures using two different low-profile attachment designs; OT- Equator attachment with and without bar attachment.

Materials And Methods: A completely edentulous epoxy resin mandibular model was used, in which two parallel dental implants were inserted at the canine region bilaterally and one in the middle. Sixteen identical complete edentulous mandibular overdentures were fabricated following conventional, standardized techniques and were divided equally between two groups according to the design and placement of the OT-Equator.

Digitization accuracy and scannability of different prosthodontic materials: An in vitro trial.

Statement Of Problem: Digital scanning of different prosthodontic materials is commonplace in contemporary practice. However, the scannability of prosthodontic materials has not been thoroughly investigated.

Purpose: The purpose of this in vitro study was to evaluate the scanning accuracy and measure the unscanned area in a preset time limit of commonly used framework materials.

Retention of zirconia crowns to titanium bases with straight versus angled screw access channels: an invitro study.

Background: The aim of this study was to assess the impact of abutments with angled screw access channel on the retention of zirconia crowns.

Methods: Seven implant replicas were inserted in epoxy resin blocks. Fourteen zirconia crowns for central incisor tooth were digitally fabricated and cemented to titanium bases (Ti-bases) with resin cement.

Evaluation of prosthetic screw loosening in all-on-four implant-supported restorations with two different connection systems.

Unlabelled: STATEMENT OF PROBLEM: The OT BRIDGE connection system can be an alternative to multiunit abutments (MUA) for patients with all-on-four implant-supported restorations. However, the amount of prosthetic screw loosening of the OT BRIDGE in comparison with the MUA used in all-on-four implant restorations is unclear.

Purpose: The purpose of this in vitro study was to compare the loss of removal torque in the absence of load and after dynamic cyclic loading between the 2 different connection systems: OT BRIDGE attachment and MUA used in all-on-four implant-supported restorations.

Evaluation of physicomechanical properties of milled versus 3D-printed denture base resins: A comparative in vitro study.

Statement Of Problem: Studies comparing the physicomechanical characteristics of denture base resins manufactured by computer-aided design and computer-aided manufacturing (CAD-CAM) milling and 3-dimensional (3D) printing are sparse, resulting in challenges when choosing a fabrication method for complete dentures.

Purpose: The purpose of this in vitro study was to evaluate and compare the impact strength, flexural strength, and the surface roughness of denture base resins manufactured by CAD-CAM milling and 3D printing before and after thermocycling and polishing.

Material And Methods: Evaluation of the physicomechanical properties (n=35) was completed before and after 500 thermocycles.

Is dextrose prolotherapy beneficial in the management of temporomandibular joint internal derangement? A systematic review.

Objective: To highlight the current knowledge of the efficacy of dextrose as a prolotherapy agent in managing temporomandibular joint internal derangement (TMJ-ID).

Methods: A "Population, Intervention, Comparison, Outcome" (PICO) strategy was executed using an electronic search through PubMed/MEDLINE, Cochrane databases, and Google Scholar from their inception to August 2022. Only randomized clinical trials investigating the treatment of TMJ-ID with hypertonic dextrose prolotherapy (HDPT) were included.

Evaluation of the virtual cement gap parameter of different CAD software programs in designing a single crown restoration: An in vitro study.

Statement Of Problem: The accuracy of different virtual cement gap parameters in designing a single crown in a computer-aided design (CAD) software program is still unclear.

Purpose: The purpose of this in vitro study was to evaluate and compare the virtual cement gap settings of 3 different CAD software programs used to design a single crown restoration.

Material And Methods: Three different CAD software programs (exocad, Dental System, and B4D) were evaluated for designing single crowns with similar virtual cement gap settings.

Carbohydrate-binding modules facilitate the enzymatic hydrolysis of lignocellulosic biomass: Releasing reducing sugars and dissociative lignin available for producing biofuels and chemicals.

The microbial decomposition and utilization of lignocellulosic biomass present in the plant tissues are driven by a series of carbohydrate active enzymes (CAZymes) acting in concert. As the non-catalytic domains widely found in the modular CAZymes, carbohydrate-binding modules (CBMs) are intimately associated with catalytic domains (CDs) that effect the diverse hydrolytic reactions. The CBMs function as auxiliary components for the recognition, adhesion, and depolymerization of the complex substrate mediated by the associated CDs.

Registration of maxillomandibular relationship through a fully digital workflow for complete-mouth rehabilitation with screw-retained fixed implant-supported prostheses: A clinical report.

Digital recording of the maxillomandibular relationship by using intraoral scanning in completely edentulous patients for computer-aided design and computer-aided manufacture implant-supported prostheses can be challenging. This clinical report describes the fabrication of complete arch, screw-retained implant-supported prostheses in a completely edentulous patient with a fully digital workflow.

Promising minimally invasive treatment modalities for symptomatic temporomandibular joint disc displacement with reduction: a randomized controlled clinical trial.

Background: Pain and clicking are the primary complaints in patients suffering from temporomandibular joint disc displacement with reduction (DDwR), negatively affecting the patients' quality of life, making the treatment essential. This prospective randomized controlled trial (RCT) was conducted to evaluate the effectiveness of botulinum toxin type-A (BTX-A) and low level laser therapy (LLLT) in comparison to anterior repositioning appliance (ARA) for the treatment of DDwR.

Methods: A total of 27 patients were randomly allocated to 3 groups; ARA (control group), BTX-A, and LLLT; with 9 patients each.

Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome.

Ruminococcus bromii is a keystone species in the human gut that has the rare ability to degrade dietary resistant starch (RS). This bacterium secretes a suite of starch-active proteins that work together within larger complexes called amylosomes that allow R. bromii to bind and degrade RS.

Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis.

Idiopathic pulmonary fibrosis is an incurable disease of unknown etiology. Acute exacerbation of idiopathic pulmonary fibrosis is associated with high mortality. Excessive apoptosis of lung epithelial cells occurs in pulmonary fibrosis acute exacerbation.

Complete Genome Sequences of Three Staphylococcus haemolyticus Strains Isolated from the Lung of a TGFβ1 Transgenic Mouse with Lung Fibrosis.

We report here the complete genome sequences of three Staphylococcus haemolyticus strains isolated from a mouse fibrotic lung tissue and exhibiting proapoptotic activity on human lung alveolar epithelial cells. The genomes were obtained from a combination of Illumina MiSeq and Oxford Nanopore MinION sequencing.

Isolation of DiNP-Degrading Microbes from the Mouse Colon and the Influence DiNP Exposure Has on the Microbiota, Intestinal Integrity, and Immune Status of the Colon.

Di-isononyl phthalate (DiNP) is a plasticizer used to impart flexibility or stability in a variety of products including polyvinyl chloride, cable coatings, artificial leather, and footwear. Previous studies have examined the impact of DiNP on gut integrity and the colonic immune microenvironment, but this study further expands the research by examining whether DiNP exposure alters the colonic microbiota and various immune markers. Previous studies have also revealed that environmental microbes degrade various phthalates, but no studies have examined whether anaerobic gut bacteria can degrade DiNP.

Comparison of the strain developed around implants with angled abutments with two reinforced polymeric CAD-CAM superstructure materials: An in vitro comparative study.

Statement Of Problem: The strain developed around implants with angled abutments should be considered when selecting a superstructure material. Studies that evaluated the strain developed around implants with angled abutments when using fiber-reinforced polymer as the implant superstructure material are lacking.

Purpose: The purpose of this in vitro study was to assess the strain developed around implants with angled abutments (15 and 25 degrees) of biocompatible high-performance polymer (BioHPP) and reinforced nanohybrid polymer with a multilayered glass fiber (TRINIA) superstructure under axial and oblique loading.

A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues.

Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cells.

Degradation Products of Complex Arabinoxylans by Enhance the Host Immune Response.

spp. of the human colonic microbiome degrade complex arabinoxylans from dietary fiber and release ferulic acid. Several studies have demonstrated the beneficial effects of ferulic acid.

Degradation of complex arabinoxylans by human colonic Bacteroidetes.

Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood.

A Staphylococcus pro-apoptotic peptide induces acute exacerbation of pulmonary fibrosis.

Idiopathic pulmonary fibrosis (IPF) is a chronic and fatal disease of unknown etiology; however, apoptosis of lung alveolar epithelial cells plays a role in disease progression. This intractable disease is associated with increased abundance of Staphylococcus and Streptococcus in the lungs, yet their roles in disease pathogenesis remain elusive. Here, we report that Staphylococcus nepalensis releases corisin, a peptide conserved in diverse staphylococci, to induce apoptosis of lung epithelial cells.

Thermophilic Degradation of Hemicellulose, a Critical Feedstock in the Production of Bioenergy and Other Value-Added Products.

Renewable fuels have gained importance as the world moves toward diversifying its energy portfolio. A critical step in the biomass-to-bioenergy initiative is deconstruction of plant cell wall polysaccharides to their unit sugars for subsequent fermentation to fuels. To acquire carbon and energy for their metabolic processes, diverse microorganisms have evolved genes encoding enzymes that depolymerize polysaccharides to their carbon/energy-rich building blocks.

Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus.

The plant cell wall polysaccharide arabinan provides an important supply of arabinose, and unraveling arabinan-degrading strategies by microbes is important for understanding its use as a source of energy. Here, we explored the arabinan-degrading enzymes in the thermophilic bacterium and identified a gene cluster encoding two glycoside hydrolase (GH) family 51 α-l-arabinofuranosidases (CpAbf51A, CpAbf51B), a GH43 endoarabinanase (CpAbn43A), a GH27 β-l-arabinopyranosidase (CpAbp27A), and two GH127 β-l-arabinofuranosidases (CpAbf127A, CpAbf127B). The genes were expressed as recombinant proteins, and the functions of the purified proteins were determined with -nitrophenyl (NP)-linked sugars and naturally occurring pectin structural elements as the substrates.