Interaction Between Malat1 and miR-499-5p Regulates Meis1 Expression and Function with a Net Impact on Cell Proliferation.

Meis1 is a transcription factor involved in numerous functions including development and proliferation and has been previously shown to harness cell cycle progression. In this study, we used in silico analysis to predict that miR-499-5p targets Meis1 and that Malat1 sponges miR-499-5p. For the first time, we demonstrated that the overexpression of miR-499-5p led to the downregulation of Meis1 mRNA and protein in C166 cells by directly binding to its 3'UTR.

A Review of COVID-19-Related Literature on Freight Transport: Impacts, Mitigation Strategies, Recovery Measures, and Future Research Directions.

The COVID-19 pandemic has caused significant disruptions in the freight transport sector. The number of studies on the impact of COVID-19 on freight transport and possible mitigation strategies are growing. However, a systematic and comprehensive review highlighting the research themes, main findings, research methods, and future research directions of these studies remains scarce.

Geospatial analysis of wetlands based on land use/land cover dynamics using remote sensing and GIS in Sindh, Pakistan.

In this study, the Land Use/Land Cover (LULC) change has been observed in wetlands comprises of Manchar Lake, Keenjhar Lake, and Chotiari Reservoir in Pakistan over the last four decades from 1972 to 2020. Each wetland has been categorized into four LULC classes; water, natural vegetation, agriculture land, and dry land. Multitemporal Landsat satellite data including; Multi-Spectral Scanner (MSS), Thematic Mapper (TM), and Operational Land Imager (OLI) images were used for LULC changes evaluation.

GAD: A Python Script for Dividing Genome Annotation Files into Feature-Based Files.

Nowadays, the manipulation and analysis of genomic data stored in publicly accessible repositories have become a daily task in genomics and bioinformatics laboratories. Due to the enormous advancement in the field of genome sequencing and the emergence of many projects, bioinformaticians have pushed for the creation of a variety of programs and pipelines that will automatically analyze such big data, in particular the pipelines of gene annotation. Dealing with annotation files using easy and simple programs is very important, particularly for non-developers, enhancing the genomic data analysis acceleration.

A genome-wide scan for loci predisposing to non-syndromic cleft lip with or without cleft palate in two large Syrian families.

Non-syndromic cleft lip with/without cleft palate (CL/P) is a common, usually non-fatal birth defect of complex etiology. Several segregation analyses have demonstrated that genetic factors are important in the pathogenesis of CL/P, most likely through the interaction of several genes of modest effects. The aim of this study was to perform a genome-wide linkage analysis to identify/search for candidate gene loci for CL/P.