Publications by authors named "Ahmed El Deeb"
Article Synopsis
- The study evaluates the effectiveness of macroscopic on-site evaluation (MOSE) during endoscopic ultrasound-guided fine-needle aspiration/biopsy (EUS-FNA/FNB) in diagnosing solid lesions, particularly focusing on histological core sample adequacy.
- Conducted across 16 medical centers in Egypt, Iraq, and Morocco, the study involved over 1000 patients and revealed that a significant majority of biopsied samples were adequate for histological evaluation, demonstrating a high diagnostic yield.
- Results indicated that FNB needles performed best in terms of sensitivity and sample quality, highlighting the potential of MOSE as a valuable alternative to traditional rapid on-site evaluation methods.
Article Synopsis
- The study evaluated the reliability and effectiveness of the Swimmer Arm-to-Shoulder (SAS) test to differentiate between shoulder impingement and cervical radiculopathy in patients with shoulder pain lasting up to 12 weeks.
- In a sample of 718 patients, the SAS test showed high accuracy, with a sensitivity of 89.2% and specificity of 96.1%, effectively identifying the underlying causes of shoulder pain.
- The results concluded that the SAS test is a simple, dependable tool for both screening and confirming diagnoses related to shoulder and cervical spine conditions.
We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5-fold, as compared to single-core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection.
View Article and Find Full Text PDFMultivalent recognition and binding of biological molecules is a natural phenomenon that increases the binding stability (avidity) without decreasing the recognition specificity. In this study, we took advantage of this phenomenon to increase the efficiency and maintain high specificity of RNA cleavage by DNAzymes (Dz). We designed a series of DNA constructs containing two Dz agents, named here bivalent Dz devices (BDD).
View Article and Find Full Text PDFAdvancements in DNA computation have unlocked molecular-scale information processing possibilities, utilizing the intrinsic properties of DNA for complex logical operations with transformative applications in biomedicine. DNA computation shows promise in molecular diagnostics, enabling precise and sensitive detection of genetic mutations and disease biomarkers. Moreover, it holds potential for targeted gene regulation, facilitating personalized therapeutic interventions with enhanced efficacy and reduced side effects.
View Article and Find Full Text PDFWe have developed a hook-equipped DNA nanomachine (HDNM) for the rapid detection of specific nucleic acid sequences without a preamplification step. HDNM efficiently unwinds RNA structures and improves the detection sensitivity. Compared to the hookless system, HDNM offers an 80-fold and 13-fold enhancement in DNA and RNA detection, respectively, reducing incubation time from 3 to 1 h.
View Article and Find Full Text PDFTherapeutic nucleic acid agents (TNA) can be activated by a marker RNA sequence followed by initiation of targeted RNA cleavage. This property can be used in conditional cell suppression, e. g.
View Article and Find Full Text PDFNucleic acid-based detection of RNA viruses requires an annealing procedure to obtain RNA/probe or RNA/primer complexes for unwinding stable structures of folded viral RNA. In this study, we designed a protein-enzyme-free nano-construction, named four-armed DNA machine (4DNM), that requires neither an amplification stage nor a high-temperature annealing step for SARS-CoV-2 detection. It uses a binary deoxyribozyme (BiDz) sensor incorporated in a DNA nanostructure equipped with a total of four RNA-binding arms.
View Article and Find Full Text PDFOligonucleotide gene therapy (OGT) agents suppress specific mRNAs in cells and thus reduce the expression of targeted genes. The ability to unambiguously distinguish cancer from healthy cells can solve the low selectivity problem of OGT agents. Cancer RNA markers are expressed in both healthy and cancer cells with a higher expression level in cancer cells.
View Article and Find Full Text PDFObjectives: To determine the value of grayscale and power Doppler ultrasound (PDUS) in the evaluation of carpal tunnel syndrome (CTS) in clinically suspected patients.
Methods: Eighty-seven wrists of 61 patients with clinically suspected CTS and 57 wrists of 30 healthy control participants were included in our study. Median nerve (MN) cross-sectional area (CSA) measurements were performed at the tunnel inlet level (wCSA) and proximal pronator quadratus muscle level (fCSA).