Association between proliferation status of infected and non-infected mononuclear cells with tissue lesions in acute bovine theileriosis.

Tropical or Mediterranean theileriosis in dairy cattle is widely distributed in many tropical regions of the world. The purpose of this study was to evaluate the proliferation status of mononuclear cells infected with schizonts in different tissues and its relationship with the pathogenesis of the parasite in cattle by histopathology, immuno-histochemistry and polymerase chain reaction (PCR). Blood and tissue samples of eight Holstein cattle that had been lost due to theileriosis and eight healthy slaughtered cattle of the same breed were collected as a control group after necropsy.

The Protective Effect of Ethanolic Extract against Infection in Immunosuppressed Mice.

Background: A specific treatment has not yet developed for cryptosporidiosis, and some of the used drugs had side effects in immunodeficient patients. The goal of an appropriate remedy is to remove symptoms and improve immune responses in hosts. The current study was designed to evaluate the therapeutic efficacy of ethanolic extract in experimentally infected immunosuppressed mice.

Vaccination with rEGVac elicits immunoprotection against different stages of Echinococcus granulosus life cycle: A pilot study.

Vaccination against dog-sheep transmission cycle is necessary to control cystic echinococcosis (CE) infection. A multi-epitope multi-antigenic recombinant vaccine was developed-comprising the three putative vaccine antigens EG95, Eg14-3-3 and EgEnolase-was cloned and expressed. In a pilot experiment, the multi-antigen vaccine was assessed in 15 dogs and 15 sheep (five experimental groups and three animals in each group) by two subcutaneous doses 28 days apart.

A multi-method and structure-based in silico vaccine designing against through investigating enolase protein.

Hydatid disease is a ubiquitous parasitic zoonotic disease, which causes different medical, economic and serious public health problems in some parts of the world. The causal organism is a multi-stage parasite named whose life cycle is dependent on two types of mammalian hosts viz definitive and intermediate hosts. In this study, enolase, as a key functional enzyme in the metabolism of (EgEnolase), was targeted through a comprehensive modeling analysis and designing a host-specific multi-epitope vaccine.

Comparative survey on scolicidal effects of selenium and silver nanoparticles on protoscolices of hydatid cyst.

Hydatidosis is a zoonotic diseases which affects human and livestock animals. The surgical treatment of hydatid diseases in man is still the most effective approach, but spillage of protoscolices is the risk of surgery. Therefore, it is essential to develop alternative approaches.

A novel B- and helper T-cell epitopes-based prophylactic vaccine against .

In this study, we targeted the worm stage of to design a novel multi-epitope B- and helper T-cell based vaccine construct for immunization of dogs against this multi-host parasite. The vaccine was designed based on the local Eg14-3-3 antigen (Ag). DNA samples were extracted from the protoscoleces of the infected sheep's liver, and then subjected to the polymerase chain reaction (PCR) with 14-3-3 specific forward and reverse primers.

A novel in silico minigene vaccine based on CD4 T-helper and B-cell epitopes of EG95 isolates for vaccination against cystic echinococcosis.

EG95 oncospheral antigen plays a crucial role in Echinococcus granulosus pathogenicity. Considering the diversity of antigen among different EG95 isolates, it seems to be an ideal antigen for designing a universal multivalent minigene vaccine, so-called multi-epitope vaccine. This is the first in silico study to design a construct for the development of global EG95-based hydatid vaccine against E.

Survey of hydatidosis infection in slaughtered camel (Camelus dromedarius) in Tabriz area, Northwest Iran.

Hydatid Disease is the name given to the condition caused by the zoonotic tapeworm Echinococcus granulosus. The tapeworm spends most of its adult life in the intestine of its definitive host, namely canids and in particular the dog. The tapeworm eggs become voided in the canids' faeces and as a result of ingesting the eggs, infection passes to the intermediate host, commonly herbivores while grazing.

The Efficacy of Ultraviolet Irradiation on Trichophyton Species Isolated From Nails.

Background: The treatment of onychomycosis is a challenge and infections are typically more severe and difficult to treat in toenails than in fingernails.

Objectives: The current study aimed to investigate the fungicidal effect of ultraviolet radiation on the growth of dermatophytes isolated from nails.

Patients And Methods: Samples from patients with clinical manifestations of onychomycosis were inoculated onto Sabouraud dextrose agar and incubated at 30°C for 14 days.

Occurrence of Linguatula serrata nymphs in cattle slaughtered in Tabriz, Iran.

The Linguatula serrata is a tongue-shaped parasite that infects carnivores or insectivorous reptile as final and herbivores as intermediate host. The aim of this study was to evaluate the prevalence of nymphal stages of L. serrata in mesenteric nodes (MLN) and mediastinal lymph nodes of cattle slaughtered in Tabriz slaughterhouse, North West of Iran.

Fecal egg counts for gastrointestinal nematodes are associated with a polymorphism in the MHC-DRB1 gene in the Iranian Ghezel sheep breed.

Genetic variation among sheep breeds in resistance to gastrointestinal nematodes (GIN) has been demonstrated in several production environments. Relationships between the ovine major histocompatibility complex and resistance to GIN have been studied, but few studies have systematically examined this issue in less-developed and semi-arid regions. The aim of the current study was to explore associations between fecal worm egg counts (FEC) for several GIN and polymorphisms in the DRB1 gene.

External parasite infection of common carp (Cyprinus carpio) and big head (Hypophthalmichthys nobilis) in fish farms of Mashhad, northeast of Iran.

Totally 75 common carp and 100 big head fishes were caught by using net from fish farms in Mashhad, northeast of Iran. In laboratory skin, eye and fin of fishes were inspected by stereomicroscope and in second phase direct smears are prepared from probable lesions. Gills were dissected and its filaments were placed in petri dishes and fixed by Glycerin.

Prevalence of Haemoproteus columbae and Trichomonas gallinae in pigeons (Columba domestica) in Isfahan, Iran.

The aim of this study was to determine the prevalence of Haemoproteus columbae and Trichomonas gallinae in pigeons (Columba domestica) in Isfahan, Iran. Study was done on 100 pigeons from January to June 2010 in Isfahan located in south of Iran. Oropharyngeal swabs were taken from all birds.

Biochemical, hematological, and electrocardiographic changes in buffaloes naturally infected with Theileria annulata.

Changes in selected blood and serum components and electrocardiography (ECG) were investigated in 20 adults (13 females and 7 males) of water buffaloes suffering from severe theileriosis. The age of all animals used in this study ranged 1.5-5 yr.