Publications by authors named "Ahmad Fatemi"

Background: Our research presents an efficient and practical method for producing Zinc Oxide nanoparticles (ZnO NPs), which have anti-leukemic effects based on ferroptosis.

Methods: The extract was employed as a capping and reducing agent for the green synthesis. The NPs have been characterized via scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy.

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  • This study investigates the link between specific HLA alleles and the risk of acute lymphoblastic leukemia (ALL) in an Iranian population.
  • Using a case-control design, researchers compared 71 ALL patients to 71 healthy individuals and employed a specialized genetic testing technique for allele identification.
  • Results indicated that HLA-DRB1*04 is associated with a higher risk for ALL, while HLA-A*26, HLA-A*33, and HLA-DRB1*03 may provide protective benefits against the disease.
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Background: Due to the high demand for novel approaches for leukemia-targeted therapy, this study investigates the impact of DNA-PK inhibitor NU7441 on the sensitivity of pre-B ALL cells to the telomerase inhibitor MST-312.

Methods: The study involved NALM-6 cells treated with MST-312 and NU7441, assessing their viability and metabolic activity using trypan blue and MTT assays. The study also evaluated apoptosis, gene expression changes, and DNA damage using flow cytometry, qRT-PCR, and micronucleus assays.

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  • * These hydrogels can transition from liquid at room temperature to gel at body temperature, making them ideal for drug delivery in eye treatments, specifically utilizing platelet-rich plasma for various ocular conditions.
  • * Results indicated that the hydrogels are biocompatible and non-toxic to ocular stem cells, enhancing the potential for improved drug delivery methods in treating eye diseases.
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Background: Acute lymphoblastic leukemia is the most prevailing pediatric hematologic malignancy, and various factors such as environmental exposures and genetic variation affect ALL susceptibility and patients outcome. According to genome-wide association studies, several single nucleotide polymorphisms (SNPs) in IKZF1 (rs4132601) and CDKN2A (rs3731249 and rs3731217) genes are associated with ALL susceptibility. Hereupon, this study aimed to discover the association between these SNPs and the risk of childhood ALL among a sample of the Iranian population.

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One of the heterogeneous hematologic malignancies of the lymphocyte precursors is ALL. ALL has two incidence peaks that were determined in 2-5 years children and 60 years old adults. Cardiotoxicity of chemotherapeutic drugs is one of important side effects which may occur during or after chemotherapy period.

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Objective: Acute lymphoblastic leukemia (ALL) is one of the most common cancers in children for which the exact pathogenesis is not yet known. Single-nucleotide variants (SNVs) in different DNA repair genes are reported to be associated with ALL risk. This study aimed to determine the association between XRCC1 (rs1799782) and NBN (rs1805794, rs709816) SNVs and childhood ALL risk in a sample of the Iranian population.

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  • The study investigates how platelet-derived microparticles (PMPs) can enhance the lifespan and cellular characteristics of mesenchymal stem cells (MSCs) by affecting key aging and longevity genes.
  • Researchers used umbilical cord MSCs and treated them with PMPs, finding that the treated cells had a shorter population doubling time and showed significant changes in gene expression related to cell aging.
  • Results suggest that PMPs could effectively extend the lifespan of MSCs by increasing the expression of genes like hTERT and c-MYC while decreasing p16, p21, and p53, but further research is needed to confirm these effects.
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An alarming increase in acute lymphoblastic leukemia cases among children and adults has attracted the attention of researchers to discover new therapeutic strategies with a better prognosis. In cancer cells, the DNA damage response (DDR) pathway elements have been recognized to protect tumor cells from various stresses and cause tumor progression; targeting these DDR members is an attractive strategy for treatment of cancers. The inhibition of the DDR pathway in cancer cells for the treatment of cancers has recently been introduced.

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  • NK cells are crucial for detecting and destroying tumor cells, but research indicates their functionality is impaired in acute myeloblastic leukemia (AML) patients.
  • This study examined the gene expression of specific NK cell receptors in 16 newly diagnosed AML patients before and after treatment, comparing them to 16 healthy controls.
  • Findings revealed a significant decrease in the activating receptor NKp46 and an increase in the inhibitory receptor KIR2DL1 in newly diagnosed patients, but after treatment, levels of KIR2DL1 and another inhibitory receptor (NKG2A) decreased, highlighting the dysfunctional NK cell activity in AML.
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  • This study investigates how high-intensity interval training (HIIT) and continuous endurance training (CET) affect diabetic cardiomyopathy, a serious complication of diabetes.
  • Using Wistar rats, the researchers measured cardiac performance and analyzed molecular changes after both training programs over 5 weeks.
  • Results indicated that HIIT was more effective than CET in lowering blood glucose and improving heart function by reducing specific miRNA and apoptotic markers associated with diabetes.
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Aims: AML (Acute myeloid leukemia) is characterized as a heterogeneous cancer. Chemokines play fundamental roles in the onset, progression cellular, migration, survival and improvement of AML therapy outcomes. The CCR5 receptors together with their ligands have indirect effects on the progression of cancer.

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Background: The Janus kinase 2 (JAK2) has an important role in the intracellular signaling in normal and neoplastic cells. JAK2 mutation, called JAK2 V617F, is frequently found in Philadelphia chromosome-negative myeloproliferative neoplasms. We aimed to assess the analytical efficiency of high-resolution melting (HRM) method using reannealing-curve analysis in comparison with routine melting-curve analysis for JAK2 V617F mutation detection.

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  • The study investigates the effects of MST-312, a telomerase inhibitor derived from EGCG, on the human multiple myeloma cell line U-266, which is significant because telomerase is often active in cancer cells but inactive in most normal cells.
  • Researchers used various techniques to assess cell viability, apoptosis, and gene expression after treating U-266 cells with MST-312.
  • The results showed that MST-312 caused cell death and apoptosis by increasing the expression of the pro-apoptotic gene Bax while decreasing anti-apoptotic and proliferative gene levels, suggesting it could be an effective treatment strategy for multiple myeloma.
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The application of conventional approaches to diabetic wound regeneration has some limitations. Thus, skin substitutes could be a new therapeutic possibility. In this regard, fibrin scaffolds are promising materials due to their desirable characteristics.

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  • Researchers explored the effects of a telomerase inhibitor, MST-312, on enhancing the effectiveness of the chemotherapy drug doxorubicin in targeting pre-B acute lymphoblastic leukemia (ALL) cells.
  • The study found that MST-312 increased apoptosis (cell death) in these leukemia cells and worked well with doxorubicin to boost its cytotoxic effects.
  • Results suggested that combining MST-312 with doxorubicin could offer a new treatment approach for patients with pre-B ALL by altering gene expressions related to cell survival and death.
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Tissue engineering has been investigated as a potential method for healing traumatized tissues. Biomaterials are material devices or implants used to repair or replace native body tissues and organs. The present study was conducted to evaluate the effects of decontamination methods on biological/mechanical properties and degradation/adhesion test of the platelet-rich fibrin (PRF) membranes to compare these properties with intact membranes as a biological biomaterial.

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Background: Acute myeloblastic leukemia (AML) is a clonal disorder due to bone marrow failure and uncontrolled proliferation of myeloid lineage. Acute promyelocytic leukemia (APL) is a subtype of AML. Heterocyclic compounds, such as indole, are considered as attractive candidates for cancer therapy, due to their abundance in nature and known biological activity.

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  • Oxidative stress from superoxide anion is a key factor in the development of coronary artery disease (CAD) and acute myocardial infarction (AMI), with NADPH oxidase complex being a major source of superoxide in blood vessels.
  • This study examined two specific variants (rs1049255 and rs4673) in the CYBA gene among 158 younger AMI patients and 168 healthy controls in Iran.
  • Results show a significant link between the rs1049255 variant and increased risk of premature AMI, while no association was found for the rs4673 variant.
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Recognition of the molecular mechanisms of cAMP action against DNA damage-induced apoptosis can be useful to improve the efficacy of DNA damaging therapeutic agents. Considering the critical role of bcl-2-associated death promoter (BAD) and p53 proteins in DNA damage -induced apoptosis, the aim of this study was to assess the effect of cAMP-elevating agents on these proteins in doxorubicin-treated pre-B acute lymphoblastic leukemia (pre-B ALL) NALM-6 cells.The pre-B ALL cell line NALM-6 was cultured and treated with doxorubicin in combination with or without cAMP-elevating agents forskolin and 3-isobutyl-1-methylxanthine (IBMX).

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  • The study explored the effects of MST-312, a telomerase inhibitor, on acute promyelocytic leukemia (APL) cells, finding it caused significant apoptosis and G2/M cell cycle arrest in a dose-dependent manner.
  • MST-312 effectively reduced telomerase activity in APL cells and inhibited the NF-κB pathway, which is involved in cell survival and proliferation, by preventing the phosphorylation and degradation of IκBα.
  • Importantly, MST-312 specifically targeted APL cells without causing harm to normal human peripheral blood mononuclear cells, indicating its potential as a targeted cancer therapy.
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  • The study investigates the relationship between IL28B polymorphism and chronic hepatitis C, focusing on genotype differences between healthy Iranians and infected patients.
  • A total of 921 chronic hepatitis C patients and 142 healthy individuals were genotyped for IL28B polymorphisms rs12979860 and rs8099917 using PCR-RFLP method; results showed no statistically significant differences in genotype distribution between the groups.
  • The research found a higher frequency of the IL28B rs12979860 CC genotype in healthy individuals compared to those with HCV genotype 1, while no significant differences were noted with HCV genotype 3.
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  • - The study aimed to investigate whether two specific platelet collagen receptor polymorphisms (GP VI T13254C and GP Ia C807T) are linked to premature acute myocardial infarction in young individuals.
  • - Researchers analyzed data from 100 young patients with myocardial infarction and 100 control subjects, finding a higher frequency of the GP Ia C807T polymorphism in patients, but no significant association with heart attacks.
  • - Ultimately, the findings concluded that neither polymorphism is associated with an increased risk of premature acute myocardial infarction, as indicated by statistical analyses.
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Aim: To investigate the structural and biochemical changes in the early stage of reperfusion in the rat livers exposed to lobar ischemia-reperfusion (IR).

Methods: The median and left lobes of the liver were subjected to 60 min ischemia followed by 5, 10, 30, 45, 60 and 120 min reperfusion. Blood samples were taken at different time intervals to test enzyme activities and biochemical alterations induced by reperfusion.

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