Molecular detection of Class 1, 2, and 3 integrons in hypervirulent and classic isolates: A cross-sectional study.

Background And Aims: The "hypervirulent" variant of (hvKp) is an emerging pathogen that cause life-threatening infection. The present study was conducted to identify the prevalence of hvKp and to investigate the presence class 1, 2, and 3 integrons in these isolates.

Methods: A cross-sectional study was conducted at three teaching hospitals, Ahvaz, South-west of Iran, from January 1, 2019 to December 31, 2020.

Design of a novel analogue peptide with potent antibiofilm activities against Staphylococcus aureus based upon a sapecin B-derived peptide.

Nowadays, antimicrobial peptides are promising to confront the existing global crisis of antibiotic resistance. Here, a novel analogue peptide (mKLK) was designed based upon a D-form amidated sapecin B-derived peptide (KLK) by replacing two lysine residues with two tryptophan and one leucine by lysine, and inserting one alanine. The mKLK displayed superior amphipathic helixes in which the most of hydrophobic residues are confined to one face of the helix and had a higher hydrophobic moment compared with KLK.

Antibiotic resistance, biofilm production ability and genetic diversity of carbapenem-resistant Pseudomonas aeruginosa strains isolated from nosocomial infections in southwestern Iran.

Background: This study was aimed to evaluate the antibiotic resistance, biofilm formation, and genetic diversity of carbapenem-resistant Pseudomonas aeruginosa (CRPA) strains isolated from four types of nosocomial infections (NIs) including urinary tract infection (UTI), ventilator-associated pneumonia (VAP), surgical site infection (SSI), and bloodstream infection (BSI).

Methods And Results: In total, 115 isolates of NIs-causing P. aeruginosa were collected from NIs.

Occurrence of plasmid-mediated quinolone resistance genes in Pseudomonas aeruginosa strains isolated from clinical specimens in southwest Iran: a multicentral study.

This study aimed to assess the presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA, and aac(6')-Ib-cr determinants as well as quinolone resistance pattern of clinical isolates of P. aeruginosa in Ahvaz, southwest Iran. A total of 185 clinical isolates of P.

Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Haemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran.

Background: This study aimed to evaluate the occurrence of Streptococcus pneumoniae and Haemophilus influenzae in sputum of patients with community-acquired pneumonia (CAP) using culture and multiplex polymerase chain reaction (M-PCR) methods and to survey the antibiotic resistance patterns of aforesaid isolates.

Result: In total, 23.9 % (n = 22/92) of sputum samples showed positive results in the culture method.

Comparative efficacy of 0.2% chlorhexidine and 10% Betadine for perioral skin disinfection prior to oral surgical procedures: a clinical trial.

Background: Surgical site infection (SSI) is a possible postoperative complication. Preoperative application of antiseptics on the surgical site can decrease the rate of SSIs.

Aim: This study aimed to compare the efficacy of 0.

The effect of tapered master gutta-percha cone on apical seal of straight and curved root canals prepared with nickel-titanium rotary files.

Background: Gutta-percha has been the predominant root canal filling material which is developed with different taper. Canal obturation fixed with nickel-titanium (NiTi) instruments and tapered gutta-percha master cone and lateral condensation is advantageous because it is clinically effectual and appears to result in a radiographically acceptable outcome. The aim of this study was to determine the effect of tapered master gutta-percha cone on apical seal of straight and curved root canals using NiTi rotary files.

Detection of Streptococcus gallolyticus in colorectal cancer and inflammatory bowel disease patients compared to control group in southwest of Iran.

There are several pieces of evidence regarding the role of bacteria, such as Streptococcus bovis/gallolyticus in the etiology of gastrointestinal diseases such as colorectal cancer (CRC) and inflammatory bowel disease (IBD). Therefore, the aim of this study was to detect S. gallolyticus subsp.

Emerge of NDM-1-Producing Multidrug-Resistant and Co-Harboring of Carbapenemase Genes in South of Iran.

Background: New Delhi metallobetalactamase1 (NDM-1) is one of the most important emerging antibiotic resistance. Co-harboring three or four carbapenemases is rare and only a few reports exist in the literature. We described the characteristics of the large epidemic outbreaks and reports co-producing with the other carbapenemase genes in isolates.

Emergence of multidrug-resistant Shigella species harboring extended-spectrum beta-lactamase genes in pediatric patients with diarrhea from southwest of Iran.

Owing to the scarce evidence about the multidrug-resistant (MDR) beta-lactamase-producing Shigella isolates in Iran, this study aimed to evaluate the occurrence of extended-spectrum beta-lactamases (ESBL) and AmpC β-lactamases in Shigella species collected in the southwest of Iran. This study was conducted on Shigella species isolated from stool samples of pediatric patients aged less than 15 years suffering from diarrhea. These isolates were identified by bacteriology tests, serotyping, and polymerase chain reaction (PCR).

Genotyping and molecular characterization of clinical isolates from a single hospital in Southwestern Iran.

() is a pathogen responsible for nosocomial infections among the hospitalized patients. The aim of this study was to investigate genotyping and molecular characterization and to examine the biofilm formation ability of isolates. In total, 70 isolates were collected from patients admitted to Imam Khomeini Hospital in Ahvaz, Southwestern Iran.

Survey on Genetic Diversity, Biofilm Formation, and Detection of Colistin Resistance Genes in Clinical Isolates of .

Introduction: is an opportunistic pathogen responsible for nosocomial infections. The emergence of colistin-resistant is a significant threat to public health. The aim of this study was to investigate the molecular characterization and genotyping of clinical isolates in Southwestern Iran.

Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of Enteroinvasive (EIEC) Isolates from Patients with Diarrhea in Ahvaz, Iran.

Background: Entero-invasive (EIEC) is one of the causes of bacillary dysentery in adults and children. The ability of EIEC to invade and colonize the surface of epithelial cells is influenced by many virulence factors. This study aimed to investigate the distribution of virulence factor genes in EIEC strains isolated from patients with diarrhea in Ahvaz, Iran, as well as the genetic diversity between these isolates by Multilocus variable-number tandem repeat analysis (MLVA).

Association between biofilm formation, structure and antibiotic resistance in isolated from neonatal septicemia in southwest Iran.

has emerged as the pathogen from neonatal septicemia. Antibiotic resistance and the capability of biofilm formation make these infections much harder to treat. Hence, the aim of this study was to investigate the association between biofilm formation, structure and antibiotic resistance in isolated from neonatal septicemia.

Isolation and characterization of from environmental and clinical sources by culture and PCR-RFLP methods.

Background And Objectives: Due to the widespread distribution of in environmental and animal sources and serious clinical complications in human, this study was aimed to isolate from water and clinical specimens by culture and PCR methods and to investigate the presence of and virulence genes.

Materials And Methods: Water and clinical samples of vaginal and fecal were screened for the presence of by phenotypic and standard biochemical tests. PCR amplification was performed on extracted DNA using primers based on the and genes.

Molecular epidemiology of colistin-resistant producing NDM-1 from hospitalized patients in Iran.

Objectives: Resistance to carbapenems is the principal reason for the continuing utilization of colistin as a last resort choice for treating the infections resulted from multidrug carbapenem-resistant (CRPA) isolates. The assessment of antimicrobial resistance pattern, the prevalence of carbapenem-resistance determinants, and molecular epidemiology of colistin-resistant isolates among CRPA strains were the aims of the present research.

Materials And Methods: The current cross-sectional research was conducted on 269 CRPA isolates collected from various clinical samples from 2013 to 2016.

Genetic basis for metronidazole and clarithromycin resistance in strains isolated from patients with gastroduodenal disorders.

Background: The aim of this study was to evaluate the antimicrobial resistance and genetic basis for metronidazole (Mtz) and clarithromycin (Cla) resistance in strains of , isolated from patients with gastroduodenal disorders.

Patients And Methods: A total of 157 . isolates (from 22 gastric cancer, 38 peptic ulcer disease, and 97 non-ulcer dyspepsia patients) were analyzed for drug susceptibility to Mtz and Cla, by gradient diffusion test (E-test, MAST).

Frequency of quinolone resistance genes among extended-spectrum β-lactamase (ESBL)-producing strains isolated from urinary tract infections.

Background: As an opportunistic pathogen, () is widely recognized as the main cause of nosocomial infections as well as some disorders especially those associated with urinary tract infections (UTIs). This study, therefore, sets out to determine the extent of antibiotic resistance to quinolones and to measure the frequency of genes (A, B, and S) within extended-spectrum beta-lactamase (ESBL) and non-ESBL-producing strains of isolated from UTI-diagnosed patients as well as to investigate their antimicrobial susceptibility patterns for some selected antibiotics in southwest Iran.

Methods: Two hundred strains were isolated from UTI-diagnosed patients, hospitalized in nine different wards of Ahvaz Golestan Hospital between November 2015 and March 2016.

Evaluation of Antibiotic Susceptibility Pattern and Distribution of coa Genes in Coagulase-negative S. aureus from Ahvaz, Iran.

Background: Staphylococcus aureus is one of the important causes of clinical infections that can be more destructive by its antibiotic resistant strains.

Objective: This study aimed to determine the antibiotic susceptibility pattern and distribution of mecA and coa genes in clinical isolates of S. aureus.