Duckweeds as edible vaccines in the animal farming industry.

Animal diseases are among the most debilitating issues in the animal farming industry, resulting in decreased productivity and product quality worldwide. An emerging alternative to conventional injectable vaccines is edible vaccines, which promise increased delivery efficiency while maintaining vaccine effectiveness. One of the most promising platforms for edible vaccines is duckweeds, due to their high growth rate, ease of transformation, and excellent nutritional content.

The Role of Silica Nanoparticles in Promoting the Germination of Tomato () Seeds.

The addition of nanoparticles has been reported to be an effective strategy for enhancing seed germination, but the underlying mechanisms whereby this occurs are unclear. In the present study, we added silica nanoparticles (SiNPs) to an aqueous growth medium in which tomato seeds were germinated. We examined the effects of SiNPs on growth and possible mechanisms of action.

Infertility and cortisol: a systematic review.

Introduction: Stress and infertility form a complex relationship. In line with this, various stress-related biological markers have been investigated in infertility.

Methods: This systematic review was performed using PRISMA guidelines (i) to report whether cortisol is highly present in infertile patients compared to fertile control; (ii) to report whether there is any significant difference in the cortisol level in infertile subjects that conceive and those that didn't at the end of assisted reproduction treatments.

A newly identified β-amyrin synthase gene hypothetically involved in oleanane-saponin biosynthesis from (Jacq.) Gaertn.

or Javanese ginseng in Indonesia is a plant widely used as a traditional medicine. The genus produces oleanane-type saponins, such as talinumoside I. The first aim of this study was to isolate the probable gene encoding β-amyrin synthase (bAS), a key enzyme involved in the cyclization of 2,3-oxidosqualene producing the backbone of the oleanane-type saponin β-amyrin and characterize the gene sequence and the predicted protein sequence using in silico approach.

Survey of understanding and awareness of fertility preservation in pediatric patients: Is conversation about fertility preservation unpleasant for pediatric patients?

Objective: To verify understanding and awareness of fertility preservation (FP) in pediatric patients undergoing FP treatments.

Methods: A questionnaire survey was conducted before and after explanation of fertility issues and FP treatments for patients 6-17 years old who visited or were hospitalized for the purpose of ovarian tissue cryopreservation (OTC) or oocyte cryopreservation (OC), or sperm cryopreservation between October 2018 and April 2022. This study was approved by the institutional review board at St.

Potential Cell-Based and Cell-Free Therapy for Patients with COVID-19.

Since it was first reported, the novel coronavirus disease 2019 (COVID-19) remains an unresolved puzzle for biomedical researchers in different fields. Various treatments, drugs, and interventions were explored as treatments for COVID. Nevertheless, there are no standard and effective therapeutic measures.

Methyl jasmonate and crude extracts of elicit agarwood compounds in shoot culture of Lamk.

Agarwood forms in the heartwood of trees in the family Thymelaeaceae in response to wounding, infection, or other stresses. Its formation is random and takes decades in natural populations, which are harvested for their aromatic compounds. This harvest has led to declining population, and many agarwood producing trees are considered endangered.

The Scenario of Adoption and Foster Care in Relation to the Reproductive Medicine Practice in Asia.

In vitro fertilization (IVF) is a hallmark of reproductive medicine. However, the inconclusive outcome leads to marital disharmonies; thus, the choices of gamete donation and surrogacy (GD/S) are often offered. In restricted countries, the child-rearing choice through foster/adoption care is promising, but the uptake remains low.

Production of bioethanol from four species of duckweeds (, , , and ) through optimization of saccharification process and fermentation with .

Duckweeds are promising potential sources for bioethanol production due to their high starch content and fast growth rate. We assessed the potential for four species, , , , and , for bioethanol production. We also optimized a possible production procedure, which must include saccharification to convert starch to soluble sugars that can serve as a substrate for fermentation.

Dataset on antixenosis and antibiosis of chili fruit by fruit fly () infestation.

This article contains the data on chili antixenosis and antibiosis to fruit fly () infestations. The data was collected from the experiment. Fifty chili varieties ( spp.

Dataset on volatile compounds in susceptible and resistant chili variety to fruit fly infestation.

This data article describes the analysis of volatile compounds in susceptible and resistant chili variety via gas chromatography/mass spectrometry (GC-MS). The volatile compounds from two chili varieties which have different level of resistance to fruit fly infestation were captured by a solid-phase micro extraction method. The obtained extracts were then subjected to GC-MS for separation and identification of the compounds in three developmental stages (buds, flowers and fruits).

Triterpenoid Saponins from Maesa argentea Leaves.

Within an ongoing research program on saponins with potential antileishmanial activity, four previously undescribed saponins were isolated from leaves and identified by LC-MS/MS, GC-MS, and 1D and 2D NMR spectroscopy as 3--{([-D-glucopyranosyl-(1 → 2)--L-rhamnopyranosyl-(1 → 2)--D-galactopyranosyl-(1 → 3)]-[-D-galactopyranosyl-(1 → 2)]--D-glucuronopyranosyl)}-21-angeloyloxy-22-butanoyloxy-13,28-oxidoolean-16,28-diol (), 3--{([-D-glucopyranosyl-(1 → 2)--L-rhamnopyranosyl-(1 → 2)--D-galactopyranosyl-(1 → 3)]-[-D-galactopyranosyl-(1 → 2)]--D-glucuronopyranosyl)}-21,22-angeloyloxy-13,28-oxidoolean-16,28-diol (), 3--{([-D-glucopyranosyl-(1 → 2)--L-rhamnopyranosyl-(1 → 2)--D-galactopyranosyl-(1 → 3)]-[-D-galactopyranosyl-(1 → 2)]--D-glucuronopyranosyl)}-21-angeloyloxy-22-()-cinnamoyloxy-13,28-oxidoolean-16,28-diol (), and 3--{([-L-rhamnopyranosyl-(1 → 2)--D-galactopyranosyl-(1 → 3)]-[-D-galactopyranosyl-(1 → 2)]--D-glucuronopyranosyl)}-21-angeloyloxy-22-()-cinnamoyloxy-13,28-oxidoolean-16,28-diol (). Leaf material was obtained from a germinated seed that was clonally propagated using tissue culturing. Compounds - showed structural similarity with maesasaponins and maesabalides reported before from other spp.

Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata.

Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M.

Unravelling the Triterpenoid Saponin Biosynthesis of the African Shrub Maesa lanceolata.

Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Candidate maesasaponin biosynthesis genes were identified through transcript profiling of M. lanceolata shoot cultures treated with methyl jasmonate, a well-known elicitor of plant specialized metabolism.

Acromioclavicular Reconstruction using Autogenous Semitendinosus Tendon Graft and the Importance of Postoperative Rehabilitation: A Case Report.

Unlabelled: We present a case of chronic acromioclavicular joint dislocation (Rockwood type 5) in which the choice of acromioclavicular reconstruction using autogenous semitendinosus tendon graft was made due to its superiority in anatomical reconstruction of the coracoclavicular ligaments, and the impact of postoperative rehabilitation on the recovery of this patient. We also discuss the rationale behind this.

Key Words: acromioclavicular joint dislocation, acromioclavicular reconstruction, autogenous semitendinosus tendon graft, rehabilitation approach.

Evaluation of the anti-angiogenic activity of saponins from Maesa lanceolata by different assays.

Angiogenesis, in which a vascular network is established from pre-existing vessels, is a complex multistep process. Mechanisms underlying angiogenesis can be investigated using a variety of in vitro, ex vivo and in vivo approaches. Evaluation of several promising plants and plant metabolites, including terpenoids, revealed promising anti-angiogenic activity.

Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata.

Unlabelled: A method has been developed to genetically transform the medicinal plant Maesa lanceolata. Initially, we tested conditions for transient expression of GFP-bearing constructs in agroinfiltrated leaves. Leaf tissues of M.

Modulation of triterpene saponin production: in vitro cultures, elicitation, and metabolic engineering.

Saponins are secondary metabolites that are widely distributed in the plant kingdom and are often the active components in medicinal herbs. Hence, saponins have a potential for the pharmaceutical industry as antibacterial, virucidal, anti-inflammatory, and anti-leishmanial drugs. However, their commercial application is often hindered because of practical problems, such as low and variable yields and limited availability of natural resources.