Hypothesis: Calcium carbonate (CaCO) nanoparticles could have great potential for contrast-enhanced ultrasound imaging (CEUS) due to their gas-generating properties and sensitivity to physiological conditions. However, the use of nano CaCO for biomedical applications requires the assistance of stabilizers to control the size and avoid the fast dissolution/recrystallization of the particles when exposed to aqueous conditions.
Experiments: Herein, we report the stabilization of nano CaCO using lignin, and synthesized core-shell amorphous CaCO-lignin nanoparticles (LigCC NPs) with a diameter below 100 nm.