Numerical analysis of acoustic impedance microscope utilizing acoustic lens transducer to examine cultured cells.

A new technique is proposed for non-contact quantitative cell observation using focused ultrasonic waves. This technique interprets acoustic reflection intensity into the characteristic acoustic impedance of the biological cell. The cells are cultured on a plastic film substrate.

Numerical analysis of ultrasound propagation and reflection intensity for biological acoustic impedance microscope.

This paper proposes a new method for microscopic acoustic imaging that utilizes the cross sectional acoustic impedance of biological soft tissues. In the system, a focused acoustic beam with a wide band frequency of 30-100 MHz is transmitted across a plastic substrate on the rear side of which a soft tissue object is placed. By scanning the focal point along the surface, a 2-D reflection intensity profile is obtained.