Mutations of microsatellite autosomal loci in paternity investigations of the Southern Poland population.

In this study, germline mutations were analyzed for 26,040 parent-child allelic transfers among subjects referred to paternity testing and originating from the Slavonic population of the Southern Poland. Mutation rates were estimated for 15 autosomal microsatellite loci: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA. There were 35 mutation events observed at 11 from 15 analyzed loci.

[Identification of rare genetic variants at DXS10074, DXS10079, DXS10146 and DXS10148 loci of investigator argus X-12 multiplex in the south Polish population].

In recent years, the analysis of X-linked short tandem repeats (X-STR), beside autosomal and Y-chromosomal STR loci, has become widely used in forensic genetic investigations. The usefulness of X-STRs markers in forensic medicine is confirmed by their high biostatistical parameters obtained for different populations. Such population studies performed on particular ethnic groups allow for demonstrating the presence of, rare genetic variants that are not included in the allelic ladders of commercially available multiplex kits.

[A rare D19S433*7 variant in the paternity case with mutation].

During a routine paternity casework performed with an automated genotyping using the AmpFISTR Identifiler kit, a lack of paternal allele segregation in D21S11 and off-ladder allele in D19S433 locus was observed. This raised suspicion of mutation because the other systems showed transmission of putative father's alleles to the child. To achieve the recommended value of paternity index (PI), the range of analysis was extended by additional autosomal loci (Penta D and Penta E) and haplotype of the chromosome Y.

[Allelic variant 6' at locus D13S317 in a paternity casework; a semi-automatic genotyping problem].

During a routine paternity casework, performed with automated genotyping using an AmpFISTR Identifiler kit, an inconsistency affecting maternal segregation of D13S317 allele was encountered, manually detected as a variant allele in the mother and child. Alleles of the putative father were transmitted in 13 out of 15 autosomal STR loci, but in CSF1PO locus, there was an apparent mutation. We, therefore, directly sequenced the variant D13S317 allele in the mother and the child and compared the results to the available data on variant alleles within this STR locus.