Depression, higher level of tension induction, and impaired coping strategies in response to stress in women with PCOS correlate with clinical and laboratory indices of hyperandrogenism and not with central obesity and insulin resistance.

Unlabelled: PCOS is characterized by ovarian hyperandrogenism and insulin resistance (IR), which give rise to symptoms of hyperandrogenism and central obesity, which in turn may cause depression, lower self-esteem, and deteriorate coping strategies in stressful situations.

The Purpose: to examine the mental condition, self-esteem, and ways of coping with stress in women with PCOS compared to age and BMI-matched healthy controls and to correlate them with clinical and laboratory hyperandrogenism, central obesity, and IR.

Methods: 42 women with PCOS and 39 controls were assessed for the above-mentioned psychological measures and correlated with serum hormonal and metabolic parameters.

Determination of urinary androgens in women with polycystic ovary syndrome using LC-QqQ/MS and the application of thin film solid-phase microextraction (TF-SPME).

Hyperandrogenism is one of the most pronounced symptoms of Polycystic Ovary Syndrome (PCOS) and seems to play a key role in the pathogenesis of this complex disorder. Nevertheless, there is still a lack of consistent results regarding common steroid predictors of PCOS. Therefore, a liquid chromatography tandem mass spectrometry (HPLC-QqQ/MS) method was developed and validated to determine the concentrations of four classic androgens: androstenedione (An-dione), testosterone (T), 5α-dihydrotestosterone (DHT) and androsterone (An) in urine samples obtained from women with PCOS and healthy controls.

Searching for the primary metabolic alterations of polycystic ovary syndrome by application of the untargeted metabolomics approach.

Despite a large number of studies, the pathogenesis of polycystic ovary syndrome (PCOS) still remains unexplained. In light of ambiguous observations reported in metabolomics, there is a need to carry out studies focusing on confirming the discriminating power of the proposed metabolomics biomarkers. Our research aimed to perform a validation study of metabolites detected in our previous study from serum samples, on the new set of samples obtained from PCOS women and healthy controls to confirm previously selected compounds.

Phytoextraction of heavy metals after application of bottom ash and municipal sewage sludge considering the risk of environmental pollution.

Waste management to reduce the loss of natural resources has become a basis of sustainable development and a circular economy. When using waste, the heavy metal (HM) concentration must be taken into account since HMs can be potentially released to the environment, posing a toxicity threat. The aim of the study was thus to estimate the availability for plants of Cr, Ni, Cu, Zn, Cd, and Pb introduced into the soil with waste.

The risk of injuries and physiological benefits of pole dancing.

Background: This study aimed to evaluate the positive and negative aspects of pole dance training.

Methods: We included 30 women trained in pole dancing and 30 untrained young women. We measured maximal hand strength, lower back and hamstring muscle flexibility, and body composition.

Identification of the metabolic fingerprints in women with polycystic ovary syndrome using the multiplatform metabolomics technique.

In addition to chronic anovulation and clinical signs of hyperandrogenism women with polycystic ovary syndrome (PCOS) are insulin resistant and therefore, develop central obesity with its long term consequences such as dyslipidaemia, hypertension, atherosclerosis and type 2 diabetes mellitus (T2DM), which all lead to the development of cardiovascular disease (CVD). Due to the polysymptomatic nature of this syndrome and lack of consensus on its diagnostic criteria there is a strong need of finding a reliable biochemical or molecular marker, which would facilitate making the accurate diagnosis of PCOS. Therefore, the aim of our study was to perform a metabolomics analysis with the use of two complementary techniques: gas chromatography and liquid chromatography coupled with mass spectrometry, of the serum samples from women with PCOS (n = 30) and to compare them with healthy age and BMI matched controls (n = 30).

Serum bisphenol A concentrations correlate with serum testosterone levels in women with polycystic ovary syndrome.

The aim of this study was to determine serum bisphenol A (BPA) concentrations using high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) in women with polycystic ovary syndrome (PCOS) (n = 106, age range 18-40 yrs) and to evaluate its potential impact on their hormonal and metabolic profile. The control group consisted of age- and BMI-matched 80 eumenorrheic women with no clinical or biochemical hyperandrogenism. Our results showed that women with PCOS had significantly higher serum BPA concentrations than healthy controls (geometric mean and [95% CI]: 0.