Chromosome aberrations and expression of ras and myc oncogenes in leiomyomas and a leiomyosarcoma of the uterus.

Twenty eight leiomyomas and one leiomyosarcoma were cytogenetically analysed and also examined for ras and myc oncoprotein expression. Chromosome alterations were found in seven leiomyoma cases. In four of them 12q14-15 was involved.

Immunohistochemical study of ras and myc oncoproteins in apocrine breast lesions with and without papillomatosis.

We have previously employed different immunohistochemical procedures for the study of breast disease in the human. The use of the monoclonal antibodies (M. Abs) against protein products of the ras and c-myc oncogenes has shown that the respective oncoproteins are expressed not only in the cancer cases but also in the majority of complex cystic diseases (C.

Immunohistochemical expression of subunit beta HCG in breast cancer.

The ectopic production of HCG by non-trophoblastic tumors is well documented. Adenocarcinomas arising in the mammary gland have been shown to stain positively for the beta subunit of HCG using immunoperoxidase technique. In the present study we used the Monoclonal antibodies (M.

Immunohistochemical detection of ras p21 and c-myc p62 in colonic adenomas and carcinomas.

In the present paper we review the results of our study on the use of monoclonal antibodies Y13,259 to the ras p21 and MYC1 9E10 to the c-myc p62 oncoprotein products in human tumors and tumorlike conditions of the colorectum. A total of 78 tissue sections were analyzed immunohistochemically and revealed the following: 42 adenocarcinomas with 8 normal mucosae adjacent to the tumor, 25 adenomas (with or without dysplasia and with in situ carcinoma) and 3 hyperplastic polyps. Parallel tissue sections were used in order to compare ras and c-myc oncoproteins.

Immunohistochemical study of the ras oncogene expression in human bladder endoscopy specimens.

We have used the monoclonal antibody Y13 259 to the ras oncogene p21 protein product in 42 endoscopy specimens from the bladder of 37 patients, in order to determine the ras oncogene expression in different conditions of the urothelium. The examined material included: 27 normal and hyperplastic or dysplastic mucosae and 13 papillomas and transitional cell carcinomas, graded according to Mostofi's classification. Our results showed the following: the normal urothelium sections tended to be negative, while the umbrella cells from the superficial layer always expressed a higher degree of positivity.

High levels of c-myc protein in human breast tumours determined by a sensitive ELISA technique.

We have previously examined the expression of (H-ras) oncogene related transcripts (Spandidos and Agnantis: Anticancer Res 4: 269-272, 1984) and also the activation of the H-ras oncogene (Spandidos: Anticancer Res 7: 991-996, 1987) in human malignant breast cancers. Our results revealed overexpression of the H-ras transcripts in malignant compared to normal tissues, while in the same tumour specimens certain point mutations and other genetic changes have been found in the H-ras gene. In this paper we have investigated c-myc oncoprotein expression in the same tumour samples using an ELISA technique (Moore et al: Oncogene Res 2: 65-80, 1989).

The reliability of histologically negative axillary lymph nodes in breast cancer. Preliminary report.

In an effort to evaluate the reliability of negative axillary lymph nodes in breast cancer patients, we did recuts in three levels in order to detect occult metastases. Our material consisted of 50 breast cancer cases with negative axilla. From each lymph node two routine sections were reviewed.

Therapeutic effect of bovine albumin in the experimental fat embolism syndrome.

The toxic effects of the free fatty acids (FFA) are responsible for the initiation and the clinical manifestations of the fat embolism syndrome (FES). Serum albumin binds to the FFA and by producing atoxic compounds it has a therapeutic effect on the syndrome. In this study we applied bovine albumin to 48 Sprague-Dawley rats which were previously submitted to intravenous infusions of FFA solutions (oleic, palmitic, stearic).

Immunohistochemical study of ras oncogene expression in endometrial and cervical human lesions.

The Y13 259 monoclonal antibody to the ras p21 protein was used in an immunohistochemical assay to study the levels of ras p21 in human uterine lesions as compared to normal tissue. Out of 73 hysterectomies obtained we have examined ras p21 expression in separately made sections from the endometrium, the cervix and leiomyomas found in the same specimens. A total of 155 tissue sections were finally evaluated and included: 55 endometrial mucosae (normal, hyperplastic and atrophic), 13 leiomyomas, 60 cervicitis (mild, moderate and severe with or without dysplasia), 3 in situ and 7 invasive carcinoma of the cervix, 12 invasive adenocarcinoma of the endometrium and 5 endometrial adenocarcinomas, which involved the cervical canal.

Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.

Expression of the c-myc gene in human breast lesions and in adjacent normal tissue was studied by immunohistochemical analysis. The previously described monoclonal antibody Myc1-9E10 (1) which recognizes the p62 c-myc protein was used in paraffin tissue sections. A total of 101 cases of breast disease examined included 38 simple and complex cystic disease, 18 simple and hyperplastic fibroadenomas, 36 ductal and lobular carcinomas and 9 in situ carcinomas.

[Breast cancer in adipose breasts].

The authors have studied breast cancer in fatty breasts in an attempt to prove their observations that Wolfe's classification "N1" on mammography was not necessarily "normal". Two groups of women, the first called group "A" consisting of 384 women with cancer of the breast, and group "B", the control group of 400 women who had no abnormality in the breast, were studied and correlated for epidemiological, thermographic, mammographic, and anatomo-pathological parameters. The following epidemiological factors were studied: age, marital status, profession, age at menarche and at menopause, menstrual behaviour, age at the first pregnancy that went to term, parity (live and still births), type of delivery, length of breast feeding, number of abortions, use of hormones, and family history of cancer of the breast.

Immunohistochemical study of the ras oncogene expression in human breast lesions.

An immunohistochemical study of ras oncogene expression in human breast lesions was carried out using a monoclonal antibody, Y13 259, to the ras encoded p21 protein. A total of 75 cases of breast disease examined included: 33 simple and complex cystic disease; 22 simple and hyperplastic fibroadenomas; 18 ductal, lobular and mixed carcinomas and 2 in situ carcinomas. Most of the complex cystic disease, hyperplastic fibroadenomas and all types of carcinomas showed high p21 expression as indicated by staining intensity.

[Is prolonged psychological stress an etiological factor in breast cancer?].

1,088 women with cancer were seen in a period of 4 1/2 years at the Cancer Centre of the Greek Social Security Department in Athens. The authors present their results as far as psychological stress influences the development of cancer of the breast in 813 patients (Group A) and in 685 women who did not have cancer of the breast (Group B). They were able to show that Group A had a positive correlation which was statistically very significant (p less than 0.

Chondrosarcoma of the thumb: an unusual case with lung and cutaneous metastases and death of the patient 6 years after treatment.

An unusual case of a chondrosarcoma developed in the proximal phalanx of the thumb is described. Although the diagnosis of the malignancy was made preoperatively and the finger was amputated without any delay, the tumor metastasized widely and killed the patient 6 years later.

Comparative study of Harvey-ras oncogene expression with conventional clinicopathologic parameters of breast cancer.

We have previously examined the expression of the Harvey-ras (Ha-ras) oncogene related transcripts in human malignant breast tumors and in their respective normal tissue [Spandidos and Agnantis, Anticancer Res. 4: 269-272, 1984]. Our results revealed a significant elevation of Ha-ras transcripts in malignant compared to normal tissue.

Human malignant tumours of the breast, as compared to their respective normal tissue, have elevated expression of the Harvey ras oncogene.

Analysis of expression of Harvey-ras related oncogenes in human malignant breast tumours and in their respective normal tissue has revealed a significant elevation of Harvey-ras transcripts in malignant as compared to normal tissue. In contrast, the sis oncogene is not expressed significantly in either type of malignant or normal tissue. These results suggest that Harvey-ras oncogene may be specifically activated in the development of human malignant breast tumours.

The reliability of frozen-section diagnosis in various breast lesions: a study based on 3451 biopsies.

In this study, we analyze breast biopsy material over a 5-year period and we compare the results of frozen-section diagnosis with the corresponding diagnosis of the permanent histologic sections. In this study, which represents the largest series in the available international literature, it is proven that frozen-section diagnosis of various breast lesions is a highly accurate procedure in spite of the difficulties facing the pathologist with the frequent borderline or premalignant lesions. The great responsibility of the pathologist is emphasized.

Correlation between serum and tissue deoxyribonuclease levels in breast cancer patients.

Acid and alkaline DNAse levels were determined in the serum of 224 breast cancer patients and in the extracts of 121 breast tissue and 48 lymph node biopsies of the above patients. The DNase levels were compared to the histological diagnoses of the tumors. Serum and tissue DNase levels were found to be significantly increased in malignancies as compared to benign conditions.

Mammary carcinoma with osteoclast-like giant cells. A study of eight cases with follow-up data.

Eight cases of a rare, distinctive variant of infiltrating mammary carcinoma featuring benign multinucleated osteoclast-like giant cells are reported. The multinucleated osteoclast-like giant cells are reported. The multinucleated giant cells were associated with ductal carcinoma in five cases and with infiltrating lobular carcinoma in three cases.