Publications by authors named "Aghigh Koochak"
Article Synopsis
- Investigations revealed weaknesses in direct sequencing and pyrosequencing for detecting mutations due to high costs and low sensitivity, leading to the exploration of high resolution melting (HRM) analysis as a rapid alternative for mutation detection.
- In a study involving 1,000 metastatic colorectal cancer patients, HRM analysis demonstrated 100% sensitivity and specificity in screening for KRAS mutations, with 336 samples identified as mutant.
- The findings suggest that HRM is a superior method compared to traditional DNA sequencing for detecting KRAS and BRAF mutations and could be beneficial for identifying mutations in other genes as well.
Background: The investigation of mutation patterns in oncogenes potentially can make available a reliable mechanism for management and treatment decisions for patients with colorectal cancer (CRC). This study concerns the rate of KRAS and BRAF genes mutations in Iranian metastatic colorectal cancer (mCRC) patients, as well as associations of genotypes with clinicopathological features.
Materials And Methods: A total of 1,000 mCRC specimens collected from 2008 to 2012 that referred to the Mehr Hospital and Partolab center, Tehran, Iran enrolled in this cross sectional study.