Molecular characterization of Salmonella enterica Serotype Typhi isolates by pulsed-field gel electrophoresis in Hong Kong, 2000-2004.

Objective: To study Salmonella enterica serotype Typhi by pulsed-field gel electrophoresis in Hong Kong.

Materials And Methods: One hundred thirty four isolates of Salmonella enterica serotype Typhi collected from 17 public hospitals and clinics in Hong Kong from 2000 to 2004 were studied in relation to epidemiological and clinical events. Isolates originated from 80 patients, with 29 patients providing multiple isolates.

Molecular subtyping of Vibrio cholerae O1 and O139 by pulsed-field gel electrophoresis in Hong Kong: correlation with epidemiological events from 1994 to 2002.

Two hundred twenty isolates of Vibrio cholerae O1 and O139 collected from 1994 to 2002 in Hong Kong were analyzed by pulsed-field gel electrophoresis (PFGE). Chromosomal DNAs from all V. cholerae isolates in agarose plugs were digested with the restriction enzyme NotI, resulting in 20 to 27 bands.