Comparison of the Intensity of Biofilm Production by Oral Microflora and Its Adhesion on the Surface of Zirconia Produced in Additive and Subtractive Technology: An In Vitro Study.

Background: This in vitro study set out to find out how well oral cavity-dwelling bacteria can form biofilms and adhere on the surfaces of zirconium oxide samples created by 3D printing and milling technologies.

Methods: 5 strains of microorganisms were used for the study, and 40 zirconium oxide samples were prepared, which were divided into two groups ( = 20)-20 samples produced using removal technology comprised the control group, while 20 samples produced by 3D printing technology comprised the test group. The prepared samples were placed in culture media of bacteria and fungi that naturally occur in the oral cavity.

Similarity Analysis of Producing Carbapenemases Isolated from UTI and Other Infections.

is an important opportunistic pathogen responsible for severe infections, mainly urinary tract infections (UTIs) and pneumonia. Hospital epidemic infections caused by multiresistant strains of carbapenemase-producing are the most concerning. NDM-producing strains are resistant to a wide range of antibiotics and have become the most significant threat.

Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain.

In our former studies based on a human whole-blood model infected with trans-anethole (TA)-treated Staphylococcus aureus Newman strain, we have observed that selected parameters/mechanisms of innate and acquired immune response were more enhanced in comparison to samples infected with non-treated bacteria. Due to this observation, the current study aimed to evaluate the concentration of selected proteins involved in both types of responses (IL-1α, IL-1β, IL-2, IL-6, IL-12, IL-17, TNF-α, IFN-γ, G-CSF, C5a, CCL1-CCL5, CXCL1, CXCL2, CXCL9-CXCL11, MMP-8, TLR2, and PGLYRP1) in healthy participants' plasma after blood stimulation of TA-treated S. aureus Newman strain.

Enhancement of neutrophil chemotaxis by trans-anethole-treated Staphylococcus aureus strains.

This study aimed to analyze the chemotactic response of differentiated HL-60 neutrophil-like (dHL-60) cells to trans-anethole (TA)-treated Staphylococcus aureus strains. Special attention was paid to evaluate the influence of TA on the chp gene expression level, as well as molecular docking and molecular dynamics (MD) simulation studies on interactions of TA with chemotaxis inhibitory protein of S. aureus (CHIPS).

Improved efficacy of eugenol and -anethole in combination with octenidine dihydrochloride against and .

Introduction And Objective: Candidiasis is a fungal infection caused by yeasts from the Ogenus Candida. Considering increasing antifungal resistance rates the activity was analyzed of natural compounds to eradicate spp. The aim of the study was to check the antifungal activity of selected essential oil compounds (EOCs; thymol, menthol, eugenol [E], carvacrol, trans-anethole [TA]) alone, and in combination with octenidine dihydrochloride (OCT) against and reference, and clinical strains.

Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing Outbreak in a Neonatal Clinic in Poland.

is one of the most common etiological agents isolated from epidemic outbreaks in neonatal wards. We describe how an extended-spectrum β-lactamase-producing (ESBL-KP) outbreak in a neonatal ward was extinguished. During the outbreak, which lasted over two months, 26 neonates were tested for , and 42 environmental swabs were taken.

Analysis of the prevalence of colistin resistance among clinical strains of .

Introduction And Objective: is an essential component of the human gut microflora. However, it can pose a threat by causing opportunistic infections, especially in hospitalised or immunocompromised patients. It is a serious problem for health medicine, primarily because of increasing resistance to previously used antibiotics.

Staphyloxanthin inhibitory potential of trans-anethole: A preliminary study.

The reduction of staphyloxanthin (STX) production in Staphylococcus aureus under trans-anethole (TA) influence was proven in former studies. However, no tests concerning the impact of TA on a biosynthetic pathway of this carotenoid pigment have been published so far. Thus, for the first time, the present preliminary study evaluated the influence of TA on the expression level of genes (crtOPQMN operon and aldH) encoding STX pathway enzymes.

Regulatory and Enterotoxin Gene Expression and Enterotoxins Production in FRI913 Cultures Exposed to a Rotating Magnetic Field and -Anethole.

The study aimed to examine the influence of a rotating magnetic field (RMF) of two different frequencies (5 and 50 Hz) on the expression of regulatory (, , ) and staphylococcal enterotoxin (SE-, , ) genes as well as the production of SEs (SEA, SEC, SEL) by the FRI913 strain cultured on a medium supplemented with a subinhibitory concentration of -anethole (TA). Furthermore, a theoretical model of interactions between the bacterial medium and bacterial cells exposed to RMF was proposed. Gene expression and SEs production were measured using quantitative real-time PCR and ELISA techniques, respectively.

Clonal Diversity, Antimicrobial Susceptibility and Presence of Genes Encoding Virulence Factors in Staphylococcus aureus Strains Isolated from Cut Wound Infections.

The aim of the study was to evaluate the clonal relatedness and antimicrobial susceptibility in 52 Staphylococcus aureus strains isolated from cut wound infections in non-related community patients and to determine the presence of selected virulence genes. To analyse the clonal relatedness of investigated strains, pulsed-field gel electrophoresis (PFGE) of macrorestricted DNA fragments was conducted. Antimicrobial susceptibility testing was performed using the AST-P644 card in the VITEK 2 Compact system.

Antibacterial and Anti-Biofilm Activities of Essential Oil Compounds against New Delhi Metallo-β-Lactamase-1-Producing Uropathogenic Strains.

The World Health Organization points out that the opportunistic pathogen that causes various infections among others, urinary tract infections (UTIs), is one of the high-priority species due to a global problem of antimicrobial resistance. The aim of this study was to investigate antibacterial and anti-biofilm activities of chosen constituents of essential oils against NDM-1-producing, uropathogenic strains. The genes encoding lipopolysaccharide (, ), adhesin gene (type I fimbriae) and gene encoding carbapenemase () for all tested strains were detected by PCR amplification.

The Impact of Intraspecies Variability on Growth Rate and Cellular Metabolic Activity of Bacteria Exposed to Rotating Magnetic Field.

Majority of research on the influence of magnetic fields on microorganisms has been carried out with the use of different species or different groups of microorganisms, but not with the use of different strains belonging to one species. The purpose of the present study was to assess the effect of rotating magnetic fields (RMF) of 5 and 50 Hz on the growth and cellular metabolic activity of eight species of bacteria: , , , , , , , and . However, contrary to the research conducted so far, each species was represented by at least four different strains.

Evaluation of Chemical Changes in Laboratory-Induced Colistin-Resistant .

This study evaluates the electrical potential and chemical alterations in laboratory-induced colistin-resistant , as compared to the susceptible strain using spectroscopic analyses. The minimal inhibitory concentration (MIC) of colistin, ζ-potential and chemical composition analysis of strains are determined. The results obtained for the with induced high-level colistin resistance (MIC = 16.

Inhibitory Effect of Eugenol and trans-Anethole Alone and in Combination with Antifungal Medicines on Candida albicans Clinical Isolates.

One of the most common pathogens among yeasts is Candida albicans, which presents a serious health threat. The study aimed to check the antifungal properties of trans-anethole and eugenol with selected antifungal medicines (AMs) against C. albicans clinical isolates.

Synergistic effect of fennel essential oil and hydrogen peroxide on bacterial biofilm.

Introduction: Staphylococcal biofilm formation significantly challenges wound management. The causes of difficult-to-treat wounds are not only methicillin-resistant staphylococci, but also methicillin-sensitive strains with different patterns of resistance. Bacterial biofilm significantly limits the access and activity of antimicrobials used in dermatological infections.

Antibacterial Activity of Selected Essential Oil Compounds Alone and in Combination with β-Lactam Antibiotics Against MRSA Strains.

This study aimed to determine the effect of selected essential oil compounds (EOCs) on the antibacterial activity of β-lactam antibiotics (βLAs) against methicillin-resistant (MRSA) strains. The following parameters were studied: antibiotic susceptibility testing, detection of gene and evaluation of genotypic relativity of isolates using molecular techniques, analysis of chemical composition applying Fourier-transform infrared (FTIR) spectroscopy, and determination of antibacterial activity of EOCs alone and in combination with βLAs against MRSA strains using microdilution and checkerboard methods. It was found that all isolates expressed MRSA and resistance phenotypes for macrolides, lincosamides, and streptogramins B.

Innate Immune Response against Preincubated with Subinhibitory Concentration of -Anethole.

The study aimed to analyze morphological and functional changes of cells due to -anethole (a terpenoid and the major constituent of fennel, anise, or star anise essential oils) exposition, and their consequences for human neutrophils phagocytic activity as well as IL-8 production (recognized as the major chemoattractant). The investigation included the evaluation of changes occurring in cultures, i.e.

The Antibacterial Activity of Lavender Essential Oil Alone and In Combination with Octenidine Dihydrochloride against MRSA Strains.

In the post-antibiotic era the issue of bacterial resistance refers not only to antibiotics themselves but also to common antiseptics like octenidine dihydrochloride (OCT). This appears as an emerging challenge in terms of preventing staphylococcal infections, which are both potentially severe and easy to transfer horizontally. Essential oils have shown synergisms both with antibiotics and antiseptics.

The Influence of Essential Oil Compounds on Antibacterial Activity of Mupirocin-Susceptible and Induced Low-Level Mupirocin-Resistant MRSA Strains.

Because of the bacterial drug resistance development, it is reasonable to investigate chemical compounds capable of preventing the spread of resistance to mupirocin (MUP), commonly used in staphylococcal eradication. The objective of the study was to verify the influence of essential oil compounds (EOCs) on the antibacterial activity of MUP against mupirocin-susceptible (MupS) and induced low-level mupirocin-resistant (MupRL) methicillin-resistant (MRSA) strains. The following parameters were examined: MRSA and MRSA susceptibility to EOCs (1,8-cineole, eugenol, carvacrol, linalool, (-)-menthone, linalyl acetate, and -anethole), the bacterial cell size distribution, and chemical composition by the use of Fourier Transform Infrared Spectroscopy (FTIR) and Raman spectroscopies.

The effect of fennel essential oil and -anethole on antibacterial activity of mupirocin against isolated from asymptomatic carriers.

Introduction: constitutes the most pathogenic species within the staphylococcal genus. Humans remain the major reservoirs of this pathogen which colonizes mostly anterior nares of healthy individuals.

Aim: To investigate the effect of fennel essential oil (FEO) and -anethole (A) on antibacterial activity of mupirocin (MUP) against strains isolated from asymptomatic carriers.

The Effect of Subinhibitory Concentrations of -Anethole on Antibacterial and Antibiofilm Activity of Mupirocin Against Mupirocin-Resistant Strains.

The aim of this study was to evaluate the effect of subinhibitory concentrations of -anethole on antibacterial and antibiofilm properties of mupirocin against mupirocin-resistant strains. Following parameters were examined: isolates susceptibility to antibiotics, minimum inhibitory concentration (MIC) of -anethole, antibacterial activity of mupirocin/-anethole combination, detection of gene, genotypic relativity of isolates using pulsed-field gel electrophoresis method, and the influence of mupirocin/-anethole combination on biofilm formation. Our study revealed that -anethole combined with mupirocin increased the growth inhibition zone diameter around the mupirocin disk, independently on strains susceptibility to this antibiotic.

Preliminary Study on the Antibacterial Activity of Essential Oils Alone and in Combination with Gentamicin Against Extended-Spectrum β-Lactamase-Producing and New Delhi Metallo-β-Lactamase-1-Producing Klebsiella pneumoniae Isolates.

Aim: The aim of the study was to investigate possible synergistic effects between several selected, commercially available essential oils and gentamicin against extended-spectrum β-lactamase (ESBL)-producing and New Delhi metallo-β-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae isolates.

Materials And Methods: ESBLs production was confirmed by double-disk synergy test. Isolates positive for bla gene were found among the tested strains.

The effect of fennel essential oil in combination with antibiotics on Staphylococcus aureus strains isolated from carriers.

An increase in the number of staphylococcal infections and carriers among medical staff has forced us to seek more and more effective antibacterial agents. Bacteria from the Staphylococcus genus possessing different mechanisms of resistance are the cause of nosocomial infections. The objective of our investigations was susceptibility of S.

[Analysis of screening tests for Streptococcus agalactiae in pregnant women from the West Pomeranian region].

Objective: The aim of the study was to determine the degree of colonization Streptococcus agalactiae (GBS--Group B Streptococcus) in women at 35.-37. weeks of pregnancy and to evaluate drug susceptibility of the isolated strains.