The evaluation of effect of selected metal ions on the efficiency of passive and active transport of imipramine.

Objectives: The aim of the study was to evaluate the effect of zinc as well as magnesium or copper ions on the efficacy of passive transport of imipramine hydrochloride in in vitro model. According to results from passive transport, the next aim of the studies was to check the efficiency of active transport of imipramine hydrochloride in the presence or absence of zinc ions.

Methods: The passive transport study was conducted in specially designed capsules, while CaCo-2 cell lines were used in active transport evaluation.

Effect of selected drugs on zinc accumulation in teeth of laboratory animals.

Background: Zinc (Zn) is a micronutrient and essential element of life and its deficiency causes severe disorders of numerous body systems, such as immune, reproductive and central nervous system. Zinc supplementation affects wound healing and sexual development. The interactions between drugs administration and Zn level in tissues are not fully understood.

The impact of ZnO and TiO on the stability of clotrimazole under UVA irradiation: Identification of photocatalytic degradation products and in vitro cytotoxicity assessment.

In order to ensure the safe and effective use of pharmaceutical products especially for topical administration photostability testing is necessary. The current paper presents an in-depth analysis of the stability of one of the most common antifungal agents, namely clotrimazole. Clotrimazole has proven to be stable under UVA irradiation in applied experimental conditions, but the presence of catalysts such as ZnO and TiO has contributed significantly to the degradation of this compound.

Remediation capacity of Cd and Pb ions by mycelia of Imleria badia, Laetiporus sulphureus, and Agaricus bisporus in vitro cultures.

The goal of this study was to evaluate cadmium and lead accumulation ability of in vitro cultures biomass containing selected edible mushroom species derived from the environment (Laetiporus sulphureus, Imleria badia) and those of commercial origin (Agaricus bisporus). Atomic absorption spectrometry was used to evaluate the content of Cd(II) and Pb(II) on the medium supplemented with Cd(II) or Pb(II), each of them at the same concentration of 5·10 M. The highest concentration of Cd(II) ions was determined in the biomass from L.

Determination of bifonazole and identification of its photocatalytic degradation products using UPLC-MS/MS.

The main goal of the presented work was to investigate the effect of ZnO or/and TiO on the stability of bifonazole in solutions under UVA irradiation. To this end, a simple and reproducible UPLC method for the determination of bifonazole in the presence of its photocatalytic degradation products was developed. Linearity was studied in the range of 0.

Determination of itraconazole and its photodegradation products with kinetic evaluation by ultra-performance liquid chromatography/tandem mass spectrometry.

A simple and reproducible UPLC-MS/MS method for the determination of itraconazole (ITZ) and its photodegradation products formed during exposure to UV-A radiation was developed. Chromatographic separations were carried out using an Acquity UPLC BEH C column (2.1 × 100 mm, 1.

Pharmacokinetics and tissue distribution of the new non-imidazole histamine H3 receptor antagonist 1-[3-(4-tert-butylphenoxy) propyl]piperidine in rats.

1. The aim of this study was to evaluate pharmacokinetics and tissue distribution of novel histamine H3 receptor antagonist 1-[3-(4-tert-butylphenoxy)propyl]piperidine (compound DL76). 2.

Binding of 1-[3-(4-tert-butyl-phenoxy)propyl]piperidine, a new non imidazole histamine H3 receptor antagonist to bovine serum albumin.

The degree of binding of a drug to plasma proteins has a significant effect on its distribution, elimination, and pharmacological effect since only the unbound fraction is available for distribution into extra-vascular space. The binding of DL76 (1-[3-(4-tert-butyl-phenoxy)propyl]piperidine) to bovine serum albumin (BSA) was studied in viitro by equilibrium dialysis at 37 degrees C and pH 7.4 over the concentration range of 0.

[Glutatione transferases--structure and functions. beta-lyase-dependent bioactivation of cysteine S-conjugates].

Glutathione transferases (GSTs) catalyze nucleophilic attack of glutathione on electrophilic center of the second substrate, hydrophobic in character. It leads to the formation of glutathione S-conjugates (thioethers), which are subsequently eliminated from the organism as mercapturic acids. However, in some reactions, glutathione can also fulfills the role of a cofactor, facilitating transformation of a hydrophobic substrate molecule, and released after the structure has been changed.

[The different aspects of the biological role of glutathione].

Glutathione plays a key role in maintaining a physiological balance between prooxidants and antioxidants, crucial for the life and death of a cell. Glutathione occurs in the human body in several redox forms, of which reduced glutathione (GSH), oxidized glutathione (GSSG), S-nitrosoglutathione (GSNO), and mixed disulfides of glutathione with proteins are the most important. There is a clear relationship between the levels of different redox forms of glutathione and the regulation of cellular metabolism in a broad sense.