Publications by authors named "Afsheen Malik"
The CRISPR-Cas9 system has recently evolved as a powerful mutagenic tool for targeted genome editing. The impeccable functioning of the system depends on the optimal design of single guide RNAs (sgRNAs) that mainly involves sgRNA specificity and on-target cleavage efficacy. Several research groups have designed algorithms and models, trained on mammalian genomes, for predicting sgRNAs cleavage efficacy.
View Article and Find Full Text PDFArticle Synopsis
- F-box proteins (FBPs) are a large group of regulatory proteins in plants, crucial for protein degradation and various biological processes, particularly in anther and pollen development, which is important for crop breeding.
- There is a need for standardized naming of FBPs, and this study proposes a systematic approach to naming and analyzing a specific sperm cell-specific FBP gene due to its high expression levels.
- The study provides insights into the gene's location, protein structure, potential functions in protein binding and ubiquitination, and its implications for improving understanding of pollen development and male fertility in hybrid breeding.