Neural progenitors derived from Tuberous Sclerosis Complex patients exhibit attenuated PI3K/AKT signaling and delayed neuronal differentiation.

Tuberous Sclerosis Complex (TSC) is a disease caused by autosomal dominant mutations in the TSC1 or TSC2 genes, and is characterized by tumor susceptibility, brain lesions, seizures and behavioral impairments. The TSC1 and TSC2 genes encode proteins forming a complex (TSC), which is a major regulator and suppressor of mammalian target of rapamycin complex 1 (mTORC1), a signaling complex that promotes cell growth and proliferation. TSC1/2 loss of heterozygosity (LOH) and the subsequent complete loss of TSC regulatory activity in null cells causes mTORC1 dysregulation and TSC-associated brain lesions or other tissue tumors.

MODERN ANTICEPTIC HYDROGEL IN TREATMENT OF INFECTIOUS COMPLICATIONS OF WOUNDS IN SURGERY.

Treatment and prevention of infectious complications remain the actual problems of surgery. Purulent complications very often arise in operative interventions with an application of various fixing devices. A study of the adhesive surgical antiseptic (ASA) «ARGAKOL» (Registration certificate № FS 012б2005/1878-05) was completed.

Transcriptome analyses of adult mouse brain reveal enrichment of lncRNAs in specific brain regions and neuronal populations.

Despite the importance of the long non-coding RNAs (lncRNAs) in regulating biological functions, the expression profiles of lncRNAs in the sub-regions of the mammalian brain and neuronal populations remain largely uncharacterized. By analyzing RNASeq datasets, we demonstrate region specific enrichment of populations of lncRNAs and mRNAs in the mouse hippocampus and pre-frontal cortex (PFC), the two major regions of the brain involved in memory storage and neuropsychiatric disorders. We identified 2759 lncRNAs and 17,859 mRNAs in the hippocampus and 2561 lncRNAs and 17,464 mRNAs expressed in the PFC.

[Substantiation of clinical application for hernioplasty of reticulate prosthesis with antimicrobial properties].

A new reticulate hernioprosthesis has been developed and proposed for clinical use. The adhesive high molecular polymer coating containing silver nanoclusters was applied on the lavsan network. After implantation the polymer was dissolved in the tissue fluid and formed the hollows between the threads facilitating invasion of the connective tissue into the network.

Influence of constant, alternating and cyclotron low-intensity electromagnetic fields on fibroblast proliferative activity in vitro.

Available data allow assuming the presence of stimulation of reparative processes under influence of low-intensity electromagnetic field, commensurable with a magnetic field of the Earth. Research of effects of low-intensity electromagnetic fields on fibroblast proliferative activity in human lungs in cell culture was performed.The influence of a constant electromagnetic field, an alternating electromagnetic field by frequency of 50 Hz and cyclotron electromagnetic field with identical intensity for all kinds of fields - 80 mcTl - on value of cellular mass and a correlation of live and dead cells in culture is investigated in three series of experiments.

[Artificial immobilization of the two-membered bacterial system Bdellovibrio bacteriovorus 109D--Escherichia coli B in polyacrylamide gel].

The interaction of a parasite with a host was studied in the two-membered bacterial system, Bdellovibrio bacteriovorus 109D and Escherichia coli B, immobilised in polyacrylamide gel (PAAG). The parasite localised inside the host cells was found to be more resistant to the toxic action of PAAG components than free B. bacteriovorus.

[Role of carbohydrate-protein recognition in the process of Bdellovibrio attaching to bacterial host cells].

The authors discuss the role of carbohydrates in the surface layer of the cell wall in the process of Bdellovibrio attachment to host-bacteria cells. The paper presents the results of inhibitory analysis using sugars conducted with two model systems as well as the data about the effectiveness of the interaction between parasite cells and host cells after the modification of their surface polysaccharide layer with concanavalin A and sodium periodate.

[Separation of exoparasitic bacteria of the genus Micavibrio from the cells and membranes of the host bacterium].

Conditions for separation of Micavibrio aeruginosavorus ARL-1 from cells and membranes of host-bacteria Pseudomonas aeruginosa have been developed. Differential centrifugation and ficoll density-gradient centrifugation were applied to purify a mixed culture. A fraction localized in the zone of 12-15% ficoll is a sufficiently homogenous suspension of the exoparasite .

[New species of exoparasitic bacteria of the genus Micavibrio infecting gram-positive bacteria].

A new species of exoparasitic bacteria, Micavibrio aeruginosavorus sp. nov., was isolated on the host bacterium, Pseudomonas aeruginosa.

[Method of determining the pteridine sensitivity of parasitic bacteria of the genus Bdellovibrio].

The method of determination of parasitic bacteria Bdellovibrio sensitivity to pteridine has been described. The method suggested can be used in research work for diagnostics of Bdellovibrio genus bacteria.

Peculiarities of the fatty acid composition of Bdellovibrio.

The fatty acid composition of twelve Bdellovibrio strains isolated upon the growth on bacteria of various taxonomic groups was studied. A dependence of the lipid composition of bdellovibrios on that of bacteria they were parasitizing on was shown. Data pointing to the selective incorporation of fatty acids of host bacteria by bdellovibrios were obtained.

[Micavibrio admirandus gen. et sp. nov].

Micavibrio gen. nov. (M.

[Comparative study of the membrane protein composition of bacteria in the genus Bdellovibrio].

The protein composition of membranes was studied in 17 Bdellovibrio strains by electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate. No similarity in the protein composition of membranes was found in the strains grown on cells of one and the same host. A dendrogram constructed basing on the similarity coefficients between the strains allowed to subdivide them into 3 groups according to the protein composition of their membranes.

[Comparative characteristics of the Bdellovibrio strains isolated from river water and sewage].

The morphology, the host ranges, the resistance to pteridine and the nucleotide composition of DNA were compared in 12 newly isolated and 10 collection strains of Bdellovibrio. The significance of properties used for the taxonomy of these organisms was evaluated. The host ranges of Bdellovibrio strains are heterogeneous with respect to the taxonomy of host bacteria.

Protein composition of Bdellovibrio bacteriovorus and Escherichia coli membranes during their interaction.

A comparative study of membrane proteins of Bdellovibrio bacteriovorus and host-bacteria Escherichia coli was performed by polyacrylamide gel electrophoresis in presence of sodium dodecyl sulphate. Infection of E. coli cells by bdellovibrions resulted in the loss of some high-molecular proteins and appearance of new ones in the host-bacteria membranes.

Detection of polyphosphates and enzymes of polyphosphate metabolism in Bdellovibrio bacteriovorus.

Bdellovibrio bacteriovorus cells, parasitizing in E. coli, contain a considerable amount of inorganic polyphosphates, 55% of the total pool of which is due to the most polymeric acid-insoluble polyphosphates. B.

[Dynamics of the interaction of Bdellovibrio bacteriovorus with the bacterial host under aerobic and anaerobic conditions].

Bdellovibrio bacteriovorus can interact with the host bacterium cells under aerobic and anaerobic conditions. The dynamics of the interaction between the parasite and the host depended on the regime of incubation. The latent period and the time at which Bd.

[Fluctuations in the numbers of Bdellovibrio bacteriovorus and host bacteria in a 2-component system].

The number of interacting organisms in a two-membered bacterial "Bdellovibrio bacteriovorus--bacterium-host" system varied within a long period of time. The variations occurred in the counterphase and had a complicated irregular character with an altering period. The Volterra principle of collisions can be applied to this type of interaction between microorganisms.

[Effect of temperature on the dynamics of interaction of Bdellovibrio bacteriovorus with host bacteria].

The dynamics of the interaction between Bdellovibrio bacteriovorus and the host bacterium was found to depend on temperature. The maximum rate of infection was found at 37 degrees C. The maximum yield of Bdellovibrio and the maximum lysis of the host cells occurred at 22.