A simple and sensitive two-tube multiplex PCR assay for simultaneous detection of ten meat species.

A sensitive and reliable technique for meat species identification is required to prevent food adulteration, particularly in meat production. This work developed an optimized multiplex PCR assay for simultaneous identification of five commonly consumed and five commonly banned meat species in meat products. We designed primers that specifically amplified mitochondrial ATPase subunit 8 gene regions of different lengths of bovine, ovine, swine, chicken, turkey, cat, dog, mouse and human DNAs.

Updates on the Production of Therapeutic Antibodies Using Human Hybridoma Technique.

Therapeutic antibodies are implicated into the very promising and fast growing area of pharmaceutics. Human hybridoma technology, allowing generation of natural human antibodies in a native form, seems to be the most direct way that require no additional modifications for production of therapeutic antibodies. However, technical difficulties in human hybridoma creation discovered in the 80s of the last century have switched the mainstream therapeutic antibody development into new directions like display and transgenic mice techniques.

[CONTENT OF OXIDATIVE STRESS MARKERS IN BLOOD PLASMA UNDER THE ACTION OF EXTRACTS OF GRATIOLA OFFICINALIS L., HELICHRYSUM ARENARIUM (L.) MOENCH, AND ANTHOCYANIN FORMS OF ZEA MAYS L].

The effect of aqueous solutions of dry ethanol extracts of Gratiola officinalis L., Helichrysum arenarium (L.) Moench, and anthocyanin forms of Zea mays L.

[Morphogenetic study of brachiopods].

In this work we discuss the results of ontogenetic and morphobiological approaches to phylogenetic reconstructions of articulate brachiopods on the basis of morphogenetic data. These data provide the basis for generalizations concerning phylogeny and system of orders. The ontogenetic approach was the subject of criticism mainly because of arbitrary establishment of the homology of brachiopods.

[Role of activation of lipid peroxidation in pathogenesis of experimental plague intoxication].

Activation of lipid peroxidation, increasing during the elevation of clinical symptoms of Y. pestis intoxication and hypoxic syndrome development, is the efferent link in cytopathogenic effects of toxic and enzymatic factors of this microorganism. Absolute or relative insufficiency of enzymatic mechanisms of blood antioxidant protection systems is the main pathogenic factor in lipid components of biomembrane destruction leading to the haemorrhagic syndrome development in Y.

[The influence of certain meteorological factors on mortality from complications of arterial hypertension].

The weather may influence the clinical course of many diseases. The objective of the present study was to evaluate effects of certain meteorological factors on the mortality rate associated with complications of arterial hypertension (cerebral stroke and myocardial infarction) in the city of Astrakhan during the period from 1983 to 2005. The analysis included 17,198 cases of death from cardiovascular disorders (CVD).

[On pathogenetic significance of activation of lipid peroxidation in the mechanisms of disturbance of blood rheologic properties in experimental intoxication induced by fraction FII of vaccinal EV strain of Y. pestis].

Changes of blood viscosity were induced by parenteral administration of fraction II of vaccinal EV strain of Y. pestis ("murine" toxin) to mice at doses equivalent to LD50 and LD25. The study revealed correlation between lipid peroxidation activity, severity of autointoxication, and integrated indices of blood theologic properties.

[Study of the quality of aminated substrates used for hybridization analysis].

A set of methods for analysis of the quality of aminated substrates that could be a basis for the large-scale manufacturing of biological microchips is suggested. The analysis includes the determination of the number of amino groups, their availability for the immobilization of phosphorylated oligonucleotides, and the characterization of surface properties of the substrates in respect to the nonspecific sorption of reagents during hybridization. A simple procedure was suggested for determination of the density/number of amino groups.

[Calcium induced calcium release from liver mitochondria of spontaneously hypertensive rats].

Background: Disturbances of ionic homeostasis of cells and recently discovered cellular energy deficiency due to reduced ATP-synthesizing ability of mitochondria are the most important components of pathogenesis of primary hypertension. Therefore it is essential to elucidate relationship between functioning of ionic transport systems especially that of calcium transport and ATP-synthesizing ability of mitochondria.

Aim: To study calcium induced calcium release from liver mitochondria of spontaneously hypertensive rats at various initial calcium concentrations in medium.

[Polymorphism of the central region of D-loop of mitochondrial DNA and personality identification by forensic medicine methods].

Polymorphism of mDNA D-loop central region (CR), positions 16366-0072) was studied versus hypervariable sections (HVS1), positions 16024-16365, and HVS2, positions 00073-00340, for a sample of 71 residents of the Russian Federation. Ten polymorphic positions with 56 nucleotide substitutions, 55 of which are transitions, were detect in the CR section; no insertions or deletions were found there. It was proven as possible to use the mDNA CR locus as an additional identification marker in the forensic-expert of the mDNA control region.

[Decreased ATP-synthesis ability of brain mitochondria in spontaneously hypertensive rats].

Unlabelled: It has been shown previously that a decrease of ATP amount and changed balance of other macroergic phosphates occurs in different tissues of spontaneously hypertensive rats (SHR) compared with control normotensive rats (WKY).

Aim: To assess the ability of SHR brain isolated mitochondria to synthesize ATP and to elucidate its relation to extramitochondrial calcium concentration.

Results: The present work shows for the first time that SHR brain mitochondria initially differ from WKY ones by decreased (by 30%) ATP synthesis rate.

Key role of the low molecular size fraction of soil humic acids for fluorescence and photoinductive activity.

The IHSS soil humic acid (HA) standard and two HAs from soils of very different origin (Chernozem and Ranker) were fractionated by tandem size-exclusion chromatography-polyacrylamide gel electrophoresis. From each HA, three fractions with different molecular sizes (MSs) and electrophoretic mobilities were obtained and investigated for their fluorescence properties and abilityto photoinduce the transformation of 2,4,6-trimethylphenol and herbicide fenuron. Regardless of the source of the HA, the two high MS fractions were found to be very weakly fluorescent.

Capillary zone electrophoresis of soil humic acid fractions obtained by coupling size-exclusion chromatography and polyacrylamide gel electrophoresis.

Capillary zone electrophoresis (CZE) was used for characterisation of soil humic acid (HA) fractions obtained by coupling size-exclusion chromatography with polyacrylamide gel electrophoresis, on the basis of their molecular size and electrophoretic mobility. CZE was conducted using several low alkaline buffers as background electrolyte (BGE): 50 mM carbonate, pH 9.0; 50 mM phosphate, pH 8.

[Distribution of HLA DQA1, LDLR, GYPA, HBGG, D7S8, and GC locus alleles in the population of Russia].

The distribution of chromosome locus alleles HLA DQA1, LDLR, GYPA, HBGG, D7S8, and GC of PolyMarker molecular genetic individualizing system was studied for the first time in a representative "mean statistical" sampling of Russian population. Typing of these locuses was carried out in 391 donors (no relatives) from 63 regions of the Russian Federation. The incidence of genotypes of all 6 locuses corresponded to the expected values, estimated on the basis of Hardy-Weinberg equilibrium hypothesis.

[Lipid peroxidation and the antioxidant blood system in dynamics of pestilential and choleraic intoxication].

Lipid peroxidation (LPO) and activity of antioxidant system were studied spectrophotometrically in white rats developing pestilential and choleraic intoxication achieved by intraperitoneal injection of plague autolysate of vaccine EB strain in dose equivalent to DL50, cholerain endotoxin in doses DL50 and DL25 as well as combined effect of choleraic endo- and enterotoxins. With progression of the intoxication, the levels of diene conjugates and malonic dialdehyde in blood plasma and erythrocytes rose. LPO activation in plague intoxication arose in high activity of SOD and blood catalase.

[Localization of disulfide bonds in the alpha 1 acidic glycoprotein molecule and study of their effect on ability of the protein to interact with ethidium bromide].

alpha 1-Acid glycoprotein (orosomucoid) was purified from the human and murine blood sera using phenol deproteinization. As opposed to the murine protein, the human orosomucoid bound the fluorescent dye ethidium bromide but lost this ability after treatment with beta-mercaptoethanol, which breaks disulfide bonds. Disulfide bonds between the Cys23 and Cys165 residues of the human orosomucoid and between the Cys91 and Cys184 residues of the murine orosomucoid were identified.

[Weight distribution and optical characteristics of soil humic acids fractionated by gel chromatography and electrophoresis].

Combination of gel chromatography on Sephadex and polyacrylamide gel electrophoresis was used to obtain molecular weight- and electrophoretic mobility-uniform fractions of humic acids from soil of different types (chernozem, sod-podzol, grey forest, and red soils). Extinction and color (E4/E6) coefficients, as well as weight distribution (%) were studied for all humic acids and their fractions. The fractions differed by the weight distribution (%).

[Effect of active medium on sclera creep].

Irreversible stretching (creep) of the eye sclera, regarded as the cause of high myopia, has been studied experimentally. Both dramatic acceleration and appreciable deceleration of the bovine sclera creep have been observed in the presence of some enzymes. Connective tissue creep caused by static and cyclic mechanical stress (gravitation, blood pressure, etc.

[Thermoinactivation of NAD-kinase from rabbit liver].

Thermal inactivation of homogeneous NAD-kinase and its partially purified preparation containing NAD-kinase in complex with glutamate dehydrogenase has been studied. The complex is more resistant to thermal inactivation in comparison with isolated NAD-kinase; its inactivation consists in irreversible dissociation preceded by a period of constant activity. No period of constant activity is observed when homogenous NAD-kinase is subjected to thermal inactivation, which represents a two-step process.

[A new two-stage plan for isolating human alpha1-acidic glycoprotein (orosomucoid). Orosomucoid interacts with ethidium bromide].

Human alpha 1-acid glycoprotein (orosomucoid, AGP) was purified to homogeneity by a two-step procedure using phenol and chloroform deproteinization of serum with subsequent preparative electrophoresis on agarose gel. It was obtained 0.15-0.

[DNA hyperproduction in the blood plasma of animals with experimental diabetes and its compensation using the transplantation of insulin-producing tissue into the anterior chamber].

Comparative analysis of serum DNA content in healthy rats, rats with experimental alloxan diabetes, and the rats with the diabetes compensated by transplantation of the embryonal pancreas into the anterior chamber of the eye. In the course of diabetes the concentration of free-circulating serum DNA reliably increase 10-12 times, and the compensation of the diabetes returns it to the norm. The revealed relationship may be used for diagnostics of initiation and full compensation of the insulin-dependent diabetes.

[The blood coagulation system under overdoses of the copolymer of 1-vinyl-3-methylimidazole iodide with the diethyl ether of maleic acid demonstrating antiheparin activity].

The effects of the active heparin antagonist--1-vinyl-3-methylimidazolium iodide-maleic acid diethyl ether copolymer--on the blood coagulative system were investigated. The effect of polycation was shown to appear only when the therapeutical dose was significantly increased and to manifest itself in the development of hypocoagulation. Hypocoagulative changes occurred only by inhibiting the activity of clotting factors V and VIII.

[Antagonistic properties of tetra-substituted analog of vasopressin with selective antidiuretic effects].

Biological properties of a novel vasopressin analogue were investigated. It was found that this analogue has no vasopressor and oxytocic activities but it exhibits a selective antidiuretic effect which is weaker than that of adiuretin (DDAVP). Novel analogue inhibits vasopressor, oxytocic and antidiuretic effects caused by arginine--vasopressin.

[The experimental effect of the copolymer of 1-vinyl-3-methyl imidazole iodide with maleic acid diethyl ester on blood coagulability].

The antiheparin activity and acute toxicity of the copolymer of 1-vinyl-3-methyl inidazol iodide (VMII) with diethyl ether of maleic acid (DEMA) were investigated. It was shown that the injection of DEMA group to the molecule of VMII polymer was adequate for storage of a high antiheparin activity of the polymer with the structure. This composition of the copolymer has a low acute toxicity and high selectivity to heparin in the blood.