Integrating CRISPR-Cas and Next Generation Sequencing in Plant Virology.

Plant pathology has been revolutionized by the emergence and intervention of next-generation sequencing technologies (NGS) which provide a fast, cost-effective, and reliable diagnostic for any class of pathogens. NGS has made tremendous advancements in the area of research and diagnostics of plant infecting viromes and has bridged plant virology with other advanced research fields like genome editing technologies. NGS in a broader perspective holds the potential for plant health improvement by diagnosing and mitigating the new or unusual symptoms caused by novel/unidentified viruses.

CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges.

Genome-editing (GE) is having a tremendous influence around the globe in the life science community. Among its versatile uses, the desired modifications of genes, and more importantly the transgene (DNA)-free approach to develop genetically modified organism (GMO), are of special interest. The recent and rapid developments in genome-editing technology have given rise to hopes to achieve global food security in a sustainable manner.

Tweaking genome-editing approaches for virus interference in crop plants.

Plant viruses infect various economically important crops and cause a serious threat to agriculture. As of now, conventional strategies employed are inadequate to circumvent the proliferation of rapidly evolving plant viruses. In this regard, recent advancement in genome-editing approach looks promising to produce plants resistant to DNA/RNA virus infections.

Assessment of variability in lignan and fatty acid content in the germplasm of L.

Information on the variability available in lignan and fatty acid content in the oilseed crop of has been limited. This article presents and discusses the composition, quantity, and variability available for the two traits in the sesame germplasm that are grown in diverse agro climatic regions of India. HPLC and GC analysis of sesame seeds harvested over a period of three crop seasons revealed a considerable amount of variability in lignan and fatty acids.

Genetic diversity and population structure of L. by using SSR markers.

The genetic variation, marker attributes and population structure was assessed in 104 genotypes of cucumber using 23 SSR primer pairs. The total number of alleles produced was 67 with an average of 2.91 per locus.

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers.

Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential.

Lignans of sesame: purification methods, biological activities and biosynthesis--a review.

Lignans are a group of compounds consisting of dimers of phenyl propane units. They are found in diverse forms distributed in a variety of plants. Sesame lignans in particular are obtained from Sesamum indicum, a highly prized oilseed crop cultivated widely in many countries in the east.