[Use of rabbit polyclonal antibodies to human annexins for recognition of inherent proteins].

Rabbit antibodies generated against annexins of human origin recognize a variety of rabbit own annexins. The antibodies under study can be divided into two groups. The first group comprises antibodies against human annexins I and IV-VI which are sufficiently specific and show only slight cross-reactivities with other proteins, whereas the second group includes antibodies against human annexins I and III which react with many different annexins and in this relation resemble consensus peptide antibodies.

Influence of the inhibition of free-radical oxidation processes in early postnatal ontogenesis on learning in adult rats.

The influence of the neonatal administration of the synthetic antioxidant mexidol on teaching rats an active avoidance conditioned reflex at the age of two months was studied. It was established that the inhibition of free-radical oxidation in the tissues of three to seven day old infant rats subsequently leads to facilitation of the learning process: to a decrease in the latent period of the avoidance reaction by a factor of 4-6, a two-fold increase in the conditioned reflex response, and a decrease in total motoric activity at the stage of the stably developed reaction.

[The effect of the inhibition of free-radical oxidation processes in early postnatal ontogeny on learning in adult rats].

The effect on neonatal administration of synthetic antioxidant mexidol on avoidance conditioning was studied in 2-month old rats. The inhibition of the free-radical oxidation in the tissues of 3-7-day old rats considerably facilitated subsequent process of learning: the avoidance response latency was reduced by 4-6 times, the conditioned response was two-fold augmented, and total motor activity was reduced at the stage of a stable response.

[The intercellular connection of axons-capillaries in the light of genetically determined resistance to emotional stress].

Genetic factor is one of the main factors of systemic organization of animal resistance to emotional stress. This notion served as a basis for comparing ultrastructural specific of connections between nervous and vascular systems in catecholamine-synthesizing areas of different lines of Wistar and August rats. Quantitative difference has been observed in the structure of cell junctions.

[The mechanisms of the reorganization of functional systems in the ontogeny of rats].

Functional systems as well as other mechanisms of regulation of animal behavior (reflexes) have very high adaptive properties. Specific mechanisms of reorganization of the functional systems have been elaborated during ontogenesis to adapt an individual to changing environment.

Calcium-dependent phospholipid binding proteins associated with the membranes of rabbit skeletal muscle.

By using extraction in the presence of Ca2+ and Triton X-100 and then in the presence of EGTA without detergent, a set of Ca2+-dependent phospholipid binding proteins has been identified in the membranes of transverse tubules (T-tubules) and sarcoplasmic reticulum (SR), isolated from rabbit skeletal muscles. Longitudinal SR, junctional SR and T-tubule membranes yielded about 9, 14 and 3.3 micrograms of EGTA-soluble proteins per 1 mg of membrane protein, respectively.

[Age-related features of dynamics of the bioelectric characteristics of command neurons in edible snail].

Differences in changes of the AP amplitude ratios induced with depolarizing current in respect to the AP in spontaneous unit activity, were found between two age groups of mollusks.

[Spatial reorganization of the organelles of catecholamine-synthesizing group A1 neurons during emotional stress].

Dynamics of spatial reorganization of organellas in catecholamine-synthesizing neurons of A1 group, that participate in reactions of the cardiovascular control, have been studied under normal conditions and under stimulation of the ventromedial nucleus in the hypothalamus. During transition of the hypertensive reaction into the hypotensive one, concentration of various organellas in the Golgi complex area and in the perinuclear zone is intensively increasing. Deep mutual nucleocytoplasmic invaginations appear.

[Influence of adequate stimulation on the impulse electrical activity of isolated frog muscle spindles].

Impulse activity of an isolated frog muscle spindle was investigated using mechanical stimulation of various amplitude, rate and form. It is found out that firing frequency during linear-increasing stimulation is determined by the amplitude of the stimulus and alteration of firing frequency by the rate of the stimulation increase. At sinusoidal stimulation the impulsation "repeates" the form of the stimulus, i.

[The effect of hypertonic solutions on the electrical activity of isolated frog muscle spindles].

Electrical activity of an isolated frog muscle spindle was investigated in hypertonic solutions obtained by adding 400 mM of sucrose, glucose or glycerine to the Ringer solution. In sucrose and glucose hypermedia the frequency of background impulse activity first increased and then decreased up to zero; receptor potentials and evoked impulse activity simultaneously decreased and disappeared. In the glycerine hypermedia impulse activity after some increase returned to normal and then a second rise of frequency to a constant supernormal level was observed.

[Retardation of growth and development of rats induced by inhibition of protein synthesis at early postnatal ontogenesis].

Treatment of rats with aurantine for 7-30 days reduces the growth and development of animals, and especially of skeletal muscles. Low relative weight of muscles in aurantine-treated animals was accompanied by low resting and action membrane potentials. Incorporation of labelled uridine and lysine into muscles, heart, brain and liver was decreased.

[Dynamic aspects of RNA metabolism in the brain of mollusca during electrical effects].

The dynamics of RNA and protein metabolism (as indicated by the incorporation of labeled precursors) during electrophysiological 'learning" and electrical stimulation of isolated ganglia of the snails Tritonia diomedia and Helix pomatia has been investigated. 'Learning" phenomenon manifested itself in the maintenance of synchronous discharges in the electrical activity of the neurons after cessation of the stimulus. At all the electrical stimulations, fast periodic processes of accumulation-degradation of RNA and protein were observed.