Plasma membranes from normal and chronic myeloid leukemic granulocytes: isolation and two-dimensional polyacrylamide gel electrophoretic analysis.

Normal human granulocytes obtained by Ficoll-Hypaque sedimentation were subjected to mild hypotonic shock and disruption by shear. The homogenate was fractionated by differential centrifugation and equilibrium ultracentrifugation to yield a plasma membrane preparation constituting 1% of the total cellular protein and enriched fifteen- and six-fold in alkaline phosphatase and Mg2+-adenosine triphosphatase activities, respectively. Granulocytes obtained from patients with chronic myeloid leukemia (CML) were identically processed.

Effect of administration of BCG, levamisole and irradiated leukemic cells on immune status and remission status in chronic myelogenous leukemia.

The immunomodulating effects of bacillus Calmette-Guérin (BCG) and levamisole with and without irradiated autologous leukemic cells were tested in patients with chronic myeloid leukemia (CML) after control of white cell count with busulfan. The response has been evaluated with respect to remission period and in vivo and in vitro immunological studies comprising delayed cutaneous hypersensitivity response to recall antigens and dinitrochlorobenzene, T cell and its subsets percentages, T-cell response to phytohemagglutinin, and to leukemia cell extracts by blastogenesis and leukocyte migration inhibition. Patients receiving BCG or levamisole did show marginal prolongation of remission, however immune parameters failed to show any improvement.

Pattern of subtypes of acute lymphoblastic leukemia in India.

Leukemic cells from 124 acute lymphoblastic leukemia (ALL) and 31 chronic lymphatic leukemia (CLL) were examined for sheep erythrocyte receptor (E), surface immunoglobulin (SIg) and their reactivity with a panel of monoclonal antibodies recognizing specific surface antigens including pan-T, Common ALL and Ia antigens. In acute lymphatic leukemia, 33% of patients reveal T-cell receptor associated with higher age group, mediastinal mass and high WBC count. Common ALL was predominant between 2 and 9-yr age group.

T-cell acute lymphoblastic leukemia: a report of 60 patients from India.

60 patients studied at the Tata Memorial Hospital from January 1976 to December 1982 were diagnosed as having T-cell acute lymphoblastic leukemia (ALL) by nonimmune rosette formation with sheep red blood cells. The analysis revealed a high incidence of mediastinal mass (50%) and extramedullary infiltration at presentation. The majority of patients (62%) presented with a high WBC count (greater than 50,000/mm3).

Distribution of subpopulations of T lymphocytes in Hodgkin's disease.

In this paper, two aspects of T cell subsets are investigated. Splenic mononuclear cells (MNC) of untreated Hodgkin's disease (HD) patients have been investigated for proportion of immunoregulatory T gamma and T mu cells from 8 uninvolved spleens and 13 involved splenic tissues from HD patients with splenic involvement. As controls, two normal spleens from accident cases were used.

Defective chemotaxis and adherence in granulocytes from chronic myeloid leukemia (CML) patients.

Adherence and motility of granulocytes from chronic myeloid leukemia (CML) patients was compared with that of granulocytes from normal subjects. The percentage of non-adherent granulocytes was significantly higher in untreated CML patients and patients in relapse and acute blastic crisis (ABC) (P less than 0.01).

The impact of combined therapeutic modalities in head, neck, and esophageal cancer.

Nearly 50% of head and neck cancers and two-thirds of patients with esophageal cancer generally present late for initial treatment. The patterns of failure are generally locoregional with around 10% showing distant dissemination. Surgery alone and in combination with pre-operative radiation has not significantly increased salvage in these groups of cancer.

Effect of retinoic acid on bone-marrow committed stem cells (CFU-c) from chronic myeloid leukemia patients.

The effect of retinoic acid (all-trans) on the clonal growth of chronic myeloid leukemia marrow was studied. The results indicate that 3 X 10(-5) M, 3 X 10(-6) M, 3 X 10(-7) M and 3 X 10(-8) M of retinoic acid inhibits the colony stimulating activity of placental conditioned medium.

Cyclic AMP metabolism in peripheral blood lymphocytes from patients with Hodgkin's disease.

Intracellular levels of cyclic AMP and cAMP phospodiesterase activity of peripheral blood mononuclear cells of patients with Hodgkin's disease (HD) were studied. The cAMP levels were elevated by 137% in HD patients as compared to normal subjects. The levels were reduced during clinical remission but remained significantly higher than normal controls.

Management of Hodgkin's disease in western India.

The authors report a retrospective analysis of 441 patients with Hodgkin's disease seen at the Tata Memorial Hospital, Bombay, over a 4-year period from 1975 to 1978. Clinicopathologic features seem to vary from those reported in the western literature, but are similar to the patterns observed in Africa and South America. Thirty-three percent of all malignant lymphomas were Hodgkin's disease.

Immunity in esophageal carcinoma.

In carcinoma of the esophagus, response to in vivo sensitization with recall antigens and DNCB was markedly depressed with 13% and 16% positivity respectively. Similarly, the number of T-cells was found to be significantly low (24 +/- 14) as compared to normal control (61 +/- 23). Blastogenesis index with PHA was only 1.

Concanavalin A induced suppressor cell activity and autorosette forming cells in chronic myeloid leukemia patients.

In the present paper attempts have been made to investigate suppressor cell activity in CML patients in first and subsequent remissions in order to study the relationship between suppressor cell activity and progression of the disease. For this purpose, the ability of Con A activated suppressor cells from peripheral blood of CML patients in 1st, 2nd and 3rd remission to suppress PHA response of autologous lymphocytes is investigated and compared with that of normal healthy donors. The ability of Con A activated cell population to form rosettes with autologous RBCs (ARFC) is also investigated.

Mixed function oxidase enzymes in human normal granulocytes and chronic myeloid leukemia cells. I. Detection and estimation.

Subcellular fractions prepared from human normal granulocytes and leukemic cells from patients with chronic myeloid leukemia (CML), were examined for 7-ethoxycoumarin O-deethylase activity as well as cytochrome P-450 and cytochrome b5 contents. The average 7-ethoxycoumarin O-deethylase activity was found to be significantly increased in all the fractions of CML cells when compared to the corresponding fractions of the normal granulocytes. The CML cells also differed from the normal cells by exhibiting decreased levels of both cytochrome P-450 and cytochrome b5.