Publications by authors named "Adrien Barbarossa"
In plant cells, a large pool of iron (Fe) is contained in the nucleolus, as well as in chloroplasts and mitochondria. A central determinant for intracellular distribution of Fe is nicotianamine (NA) generated by NICOTIANAMINE SYNTHASE (NAS). Here, we used Arabidopsis thaliana plants with disrupted NAS genes to study the accumulation of nucleolar iron and understand its role in nucleolar functions and more specifically in rRNA gene expression.
View Article and Find Full Text PDFArticle Synopsis
- The survival motor neuron (SMN) complex and the methylosome, which includes the pICln protein, play a crucial role in the production of snRNPs necessary for mRNA splicing.
- A genetic study of the Schizosaccharomyces pombe (yeast) homolog of the pICln protein shows that while not essential, SpICln is important for yeast growth and successfully complements the slow-growth phenotype when replaced with the human gene.
- The research indicates that SpICln is necessary for efficient splicing and snRNP production, while also suggesting that changes in ICln activity cannot fix the growth issues seen in cells lacking SMN, highlighting the delicate balance required in splice
The survival of motor neuron (SMN) protein plays an important role in the biogenesis of spliceosomal snRNPs and is one factor required for the integrity of nuclear Cajal bodies (CBs). CBs are enriched in small CB-specific (sca) RNAs, which guide the formation of pseudouridylated and 2'-O-methylated residues in the snRNAs. Because SMN-deficient cells lack typical CBs, we asked whether the modification of internal residues of major and minor snRNAs is defective in these cells.
View Article and Find Full Text PDF