Coexisting Molecular Determinants of Acquired Oxaliplatin Resistance in Human Colorectal and Ovarian Cancer Cell Lines.

Oxaliplatin (OHP) treatment of colorectal cancer (CRC) frequently leads to resistance. OHP resistance was induced in CRC cell lines LoVo-92 and LoVo-Li and a platinum-sensitive ovarian cancer cell line, A2780, and related to cellular platinum accumulation, platinum-DNA adducts, transporter expression, DNA repair genes, gene expression arrays, and array-CGH profiling. Pulse (4 h, 4OHP) and continuous exposure (72 h, cOHP) resulted in 4.

Singlet Oxygen Sensor Green is not a Suitable Probe for O in the Presence of Ionizing Radiation.

A great number of fluorescent probes have been developed for detecting singlet oxygen (O), which is considered to be one of the most effective reactive oxygen species (ROS), especially in clinical applications. The commercially available fluorescent probe Singlet Oxygen Sensor Green (SOSG) is widely used due to its reported high selectivity to O. In this study, we carried out systemic experiments to determine the activation of SOSG in the presence of ionizing radiation.

Cryogenic transmission electron microscopy: the technique of choice for the characterization of polymeric nanocarriers.

This letter is meant to make scientists aware of the proper application of transmission electron microscopy (TEM) for the assessment of polymeric self-assemblies. Cryogenic (cryo)-TEM should be the method of choice. Here, we show the difference in morphologies observed in the same sample when using cryo-TEM and when using TEM with drying, demonstrating the importance of choosing the proper method.

Radiolabeling polymeric micelles for in vivo evaluation: a novel, fast, and facile method.

Background: Single photon emission computed tomography (SPECT) is an indispensable tool in the determination of the in vivo fate of polymeric micelles. However, for this purpose, the micelles need to be radiolabeled, and almost all radiolabeling procedures published to date involve the conjugation of a chelating agent to the constituting polymer, which could actually affect their biodistribution. In this paper, we report a new facile method for radiolabeling polystyrene-b-poly(ethylene oxide) diblock copolymer micelles without the necessity of any chemical modification.