Assessment of 10-MDP and GPDM monomers on viability and inflammatory response in human dental pulp stem cells.

Objectives: to assess the cytotoxicity of the following functional monomers used in dental adhesives: 10-Methacryloyloxydecyl dihydrogen phosphate (10-MDP) and glycerol phosphate dimethacrylate (GPDM), and their effect on cytokine release from human dental pulp stem cells (hDPSCs).

Methods: The hDPSCs cells were isolated from the dental pulp of extracted human third molars. The functional monomers, 10-MDP and GPDM, were diluted in dimethyl sulfoxide (DMSO) at concentrations ranging from 1 to 4 mM.

Inflammatory and adhesion profile of gingival fibroblasts to lithium disilicate ceramic surfaces.

Objectives: Lithium disilicate (LS) ceramic emerges as a compelling option for customized implant abutments. However, ensuring its safety and reliability requires clarification on key aspects, notably its impact on inflammation and potential for cell adhesion. This study delves into these considerations, examining the influence of LS ceramic on cytokine release and the transcriptional profile of human gingival fibroblasts (hGFs) in direct contact with various LS surfaces.

Guidance on the assessment of biocompatibility of biomaterials: Fundamentals and testing considerations.

Background: Assessing the biocompatibility of materials is crucial for ensuring the safety and well-being of patients by preventing undesirable, toxic, immune, or allergic reactions, and ensuring that materials remain functional over time without triggering adverse reactions. To ensure a comprehensive assessment, planning tests that carefully consider the intended application and potential exposure scenarios for selecting relevant assays, cell types, and testing parameters is essential. Moreover, characterizing the composition and properties of biomaterials allows for a more accurate understanding of test outcomes and the identification of factors contributing to cytotoxicity.

Novel co-initiators of polymerization: Cytotoxicity profile and modulation of inflammatory mediators in human dental pulp stem cells.

Objectives: The aim of this study was to assess the cytotoxicity of novel polymerization co-initiators and their effect on cytokine release from human dental pulp stem cells (hDPSCs), comparing them with commonly used co-initiators.

Methods: Cells were isolated from the dental pulp of healthy human third molars. The new co-initiators, namely HDa1, HD4, HD1, and MHPTm, were evaluated and compared with the compounds dimethylaminoethyl amine benzoate (EDAB) and 2-(dimethylamino)ethyl methacrylate (DMAEMA).

Achieving property enhancements in dental resin composites via reduced concentrations of camphorquinone within a ternary initiator system.

Objectives: The study aimed to assess the impact of diphenyliodonium hexafluorophosphate (DPI) on the physicochemical properties of experimental resin composites (ECRs) featuring reduced concentrations of camphorquinone (CQ)/amine.

Methods: Five concentrations of CQ (0.125, 0.

Evaluating antimicrobial, cytotoxic and immunomodulatory effects of glass ionomer cement modified by chitosan and hydroxyapatite.

Objectives: This study aimed to assess antimicrobial efficacy, cytotoxicity, and cytokine release (IL-1b, IL-6, IL-10, TNF-α) from human dental pulp stem cells (hDPSCs) of chitosan (CH) and hydroxyapatite (HAp)-modified glass ionomer cements (GIC).

Methods: GICs with varied CH and HAp concentrations (0 %, 0.16 %, 2 %, 5 %, 10 %) were tested against S.

Toxicity and cytokine release from human dental pulp stem cells after exposure to universal dental adhesives cured by single peak and polywave LEDs.

Objectives: to assess the impact of universal adhesives, cured with single-peak and polywave LEDs, on the metabolic activity and cytokine release of human dental pulp stem cells (hDPSCs). In addition, analyze the degree of conversion (DC) of the adhesives cured with the different LEDs.

Methods: Discs (5 mm diameter, 1 mm thick) were prepared using three universal adhesives: Single Bond Universal (SBU, 3 M ESPE), Optibond Universal (OBU, Kerr), and Zipbond Universal (ZBU, SDI).

Interaction of different concentrations of 10-MDP and GPDM on the zirconia bonding.

Objectives: To analyze the influence of different concentrations of 10-MDP and GPDM used, combined or not, on the bonding to zirconia.

Methods: Specimens of zirconia and a resin-composite (7 mm length, 1 mm width, and 1 mm thick) were obtained. The experimental groups were obtained according to the functional monomer (10-MDP and GPDM) and the concentrations (3 %, 5 %, and 8 %).

Identifying markers volatiles in Brazilian virgin oil by multiple headspace solid-phase microextraction, and chemometrics tools.

A protocol was optimized to determine the volatile profile from monovarietal virgin olive oil (VOO) by multiple headspace solid-phase microextraction (MHS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis. For this, a Plackett-Burman (PB) and central composite rotational designs (CCRD) were used to define the best condition of extraction. Moreover, fatty acids profile and principal component analysis (PCA) was used to identify markers among the cultivars.

Co-initiators of polymerization can modulate the inflammatory cytokine release without major cytotoxic effects in human dental pulp cells.

To evaluate the cytotoxicity of co-initiators of polymerization and its influence on cytokine release from human dental pulp cells (hDPCs). Cells were isolated from the dental pulp of sound human third molars. The co-initiators dimethylaminoethyl amine benzoate-(EDAB), 2-(dimethylamino)ethyl methacrylate (DMAEMA); 2-Ethylhexyl 4-(dimethylamino)benzoate (EHA) and bis(4-methyl phenyl)iodonium hexafluorophosphate (BPI) were diluted in dimethylsulfoxide (DMSO) at different concentrations.

Influence of different concentrations of an iodonium salt on properties of amine-free resins.

Objectives: to evaluate the effect of different concentrations of an iodonium salt on the degree of conversion, polymerisation rate, flexural strength and modulus of an amine-free photopolymerizable resin system.

Methods: Two series of UDMA/TEGDMA resin mixtures (1:1 mass ratio) containing 0, 0.5, 1.

Physicochemical properties of dental resins formulated with amine-free photoinitiation systems.

Objective: To assess the mechanical properties of two different dimethacrylate resin blends containing the photosensitizer camphorquinone (CQ) alone or in combination with one or more synergists including an amine and/or an iodonium.

Methods: Two co-monomer resin blends were formulated using Bis-GMA/TEGDMA and UDMA/TEGDMA, both at 1:1 mass ratio. Each resin blend was divided into four groups, comprising the following four photoinitiation systems: (1) CQ + 2-(dimethylamino)ethyl methacrylate (DMAEMA); (2) CQ + DMAEMA + bis(4-methyl phenyl)iodonium hexafluorophosphate (BPI); (3) CQ; and (4) CQ + BPI.

Positive Synergistic Effect of Adhesives and Resin Cements Containing an Iodonium Salt on Bonding of Fiber Posts.

Purpose: To evaluate the push-out bond strength (PBS) of glass-fiber posts (GFP) in different root canal zones (cervical, middle, and apical), bonded with experimental simplified adhesives (ESAs) containing different initiator systems (camphorquinone [CQ] or phenylpropanodione [PPD]) with or without diphenyl iodonium hexafluorophosphate (DPI), in combination with a DPI-containing composite cement.

Materials And Methods: ESA blends were prepared with bisphenol glycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEG-DMA), 1,3-glycerol dimethacrylate (GDMA), 2-hydroxyethyl methacrylate (HEMA), and ethanol, then divided into 12 experimental groups (n = 10) according to the initiator systems (CQ, PPD, or CQ + PPD) and the presence or absence of DPI. The roots of 120 extracted bovine incisors were prepared with #5 Largo drills and the GFP were cemented with each ESA and experimental composite cements containing 0.

Transmission of violet and blue light and current light units through glass-reinforced ceramics with different thicknesses.

Purpose To evaluate the effect of glass-reinforced ceramics (leucite and lithium disilicate) with different thicknesses (1, 2, and 3 mm) on the wavelength and irradiance spectrum of blue and violet lights. In addition, the effect of the ceramics on four current light-curing units (LCUs) was evaluated: a halogen lamp, a single peak LED, and two multi-peak LEDs.Methods Ceramic discs of different thicknesses (1, 2, and 3 mm) were obtained from computer-aided design and computer-aided manufacturing (CAD-CAM) blocks.

Chemistry of novel and contemporary resin-based dental adhesives.

The chemistry of resin-based dental adhesives is critical for its interaction with dental tissues and long-term bonding stability. Changes in dental adhesives composition influences the materials' key physical-chemical properties, such as rate and degree of conversion, water sorption, solubility, flexural strength and modulus, and cohesive strength and improves the biocompatibility to dental tissues. Maintaining a suitable reactivity between photoinitiators and monomers is important for optimal properties of adhesive systems, in order to enable adequate polymerisation and improved chemical, physical and biological properties.

Viscosity and thermal kinetics of 10 preheated restorative resin composites and effect of ultrasound energy on film thickness.

Objective: This study investigated viscosity and thermal kinetics of 10 selected preheated restorative resin composites and the effect of ultrasound energy on film thickness.

Methods: A range of different resin composites was tested: Charisma Diamond, IPS Empress Direct, Enamel Plus HRi, Essentia, Estelite Omega, Filtek Z100, Filtek Z350 XT, Gradia, TPH Spectrum and VisCalor. A flowable resin composite (Opallis Flow) and two resin cements (RelyX Veneer, Variolink Esthetic LC) also were tested.

Bis(4-methyl phenyl)iodonium as an alternative component to diphenyliodonium in camphorquinone-based ternary initiating systems.

Objective: To evaluate the influence of different co-initiators (diphenyliodonium hexafluorophosphate - DPI - and bis(4-methyl phenyl)iodonium hexafluorophosphate - BPI) on chemical and mechanical properties of resins.

Methods: Nine experimental resins (50% Bis-GMA and 50% TEGDMA, w/w) with 60 wt% filler particles were formulated. The initiating system used was camphorquinone (CQ-1 mol%) and ethyl dimethylaminobenzoate (EDAB-2 mol%).

The influence of nanofillers on the properties of ethanol-solvated and non-solvated dental adhesives.

Objectives: The aim of this study was to evaluate the influence of different concentrations of nanofillers on the chemical and physical properties of ethanol-solvated and non-solvated dental adhesives.

Materials And Methods: Eight experimental adhesives were prepared with different nanofiller concentrations (0, 1, 2, and 4 wt%) and 2 solvent concentrations (0% and 10% ethanol). Several properties of the experimental adhesives were evaluated, such as water sorption and solubility ( = 5, 20 seconds light activation), real-time degree of conversion (DC; = 3, 20 and 40 seconds light activation), and stability of cohesive strength at 6 months (CS; = 20, 20 seconds light activation) using the microtensile test.

Solvent and HEMA Increase Adhesive Toxicity and Cytokine Release from Dental Pulp Cells.

The aim of the present study was to evaluate the effect of the hydroxyethyl-methacrylate (HEMA) concentration and solvent content of dental adhesives on cell viability and cytokine (IL-1b, IL-6, IL-10, TNF-α) release by human dental pulp cells (HDPCs). HDPCs were obtained from fresh extracted human third molars. Experimental adhesives were prepared containing different concentrations of HEMA (0%, 10%, and 20%) with and without solvent (ethanol 10%).

Increased rates of photopolymerisation by ternary type II photoinitiator systems in dental resins.

To evaluate the effects of Type I and Type II photoinitiator systems on curing efficiency, degree of conversion (DC) and chemico-physical properties of resin based materials. A comonomer base containing 50%wt 2.2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phenyl]propane (bis-GMA) and 50%wt triethyleneglycol dimethacrylate (TEGDMA) was formulated with either 0.

Light curing resin cements containing iodonium salts promote suitable apical bonding of posts to radicular dentin.

The aim of this study was to analyze the efficiency of experimental light-curing resin cements (ERCs) with a ternary photo-initiator system containing diphenyliodonium hexafluorphosphate (DPI) and different amines on retention of glass-fiber posts to dentin (GFP). ERCs formulations: a 1:1 mass ratio of 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phenylpropane and triethyleneglycol dimethacrylate. Camphorquinone was used as initiator.