Prevalence and Morphological Appearance of Sperm Nuclear Defects Using Feulgen Reaction in Beef Bulls.

The objective of this study was to assess the prevalence and compare the morphological aspects of sperm nuclei abnormalities in beef bulls submitted to breeding soundness evaluation (BSE). Semen samples were collected by electroejaculation from 649 beef bulls (Bos indicus, n = 515 and Bos indicus × Bos taurus crossbreed, n = 134) ranging from 2 to 12 years of age. Following a clinical evaluation and semen assessment, a slide was prepared with fresh semen and stained by using the Feulgen-stain for the purpose of evaluating sperm and nuclear morphology.