Polyphasic identification of Vibrio species from aquatic sources using mass spectrometry, housekeeping gene sequencing and whole genome analysis.

Accurate bacterial identification is essential for determining the causative agent of an infection, thus facilitating appropriate treatment and management strategies in both human and animal health contexts. Some species in the Vibrio genus are recognized pathogens, associated with a variety of infections. However, identification of these bacteria is oftentimes controversial.

The gut microbiota of wild birds undergoing rehabilitation as a reservoir of multidrug-resistant enterococci in a metropolitan area in Brazil.

Enterococci are ubiquitous usually commensal bacteria that can act as opportunistic pathogens frequently associated with resistance to multiple antimicrobial classes. A variety of animals may carry potentially harmful enterococci. In the present work, the occurrence and characteristics of enterococci recovered from the fecal microbiota of wild birds belonging to four families (Accipitridae, Cathartidae, Falconidae and Strigidae) were investigated.

Draft Genome Sequences of Four Enterococcus cecorum Strains Isolated from American Black Vultures (Coragyps atratus) and Harris's Hawk (Parabuteo unicinctus) in Rio de Janeiro, Brazil.

We report the draft genome sequences of four Enterococcus cecorum strains obtained from cloacal swab specimens collected from three healthy captive wild birds (two Coragyps atratus and one Parabuteo unicinctus) in Rio de Janeiro, Brazil. The genome sizes ranged from 2.38 to 2.

Draft Genome Sequences of Two Commensal Enterococcus faecalis Strains Isolated from American Black Vultures (Coragyps atratus) in Brazil.

We report the draft genome sequences of two commensal Enterococcus faecalis strains (designated Ca-2 and Ca-18) recovered from the cloacae of two healthy American black vultures (Coragyps atratus) in Rio de Janeiro, Brazil. The strains were found to carry a variety of antimicrobial resistance and virulence-associated genes.

Expression of VanA-type vancomycin resistance in a clinical isolate of Enterococcus faecium showing insertion of IS19 in the vanS gene.

The characteristics of an unusual clinical isolate of Enterococcus faecium (CL-6729) showing insertion of IS19 (also known as ISEfm1) in the vanS gene while maintaining a constitutive VanA phenotype are described. This isolate was obtained from a hospital-acquired urinary tract infection, showed multidrug resistance by antimicrobial susceptibility testing, and belongs to ST78 based on multilocus sequence typing (MLST). Except for the vanS gene, all the other genes of the vanA gene cluster were intact according to conventional PCR, overlapping PCR and genome sequencing.

Draft Genome Sequence of a Wohlfahrtiimonas chitiniclastica Strain Isolated from Frozen Chicken in Rio De Janeiro, Brazil.

Here, we report the draft genome sequence of strain 20, isolated from a chicken carcass originated from indoor broiler farming and identified using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry followed by sequencing of the 16S rRNA gene.

Draft Genome Sequence of Multidrug-Resistant Enterococcus faecium Strain E1298, with a Sequence Type 1274 Profile, Recovered from the Cloacal Microbiome of a Tropical Screech Owl (Megascops choliba) in Rio de Janeiro, Brazil.

Here, we present the draft genome sequence of strain E1298, a representative of the clonal complex 17 (CC17), identified as sequence type 1274 (ST1274) and resistant to multiple classes of antimicrobials, isolated from the cloaca of a tropical screech owl () in Rio de Janeiro, Brazil.

Draft Genome Sequence of Enterococcus faecium CL-6729, a Clinical Isolate Showing Constitutive Vancomycin Resistance.

Here, we present the draft genome sequence of an unusual Enterococcus faecium isolate (CL-6729) showing constitutive expression of the VanA type of vancomycin resistance. The isolate was recovered from a patient with a nosocomial urinary tract infection in Brazil.