Immunological identification of a cAMP-dependent protein kinase regulatory subunit-like protein from the Trypanosoma equiperdum TeAp-N/D1 isolate.

Polyclonal immunoglobulin Y (IgY) antibodies were produced in chicken eggs against the purified R(II)-subunit of the cAMP-dependent protein kinase (PKA) from pig heart, which corresponds to the Sus scrofa R(II)α isoform. In order to evaluate whether Trypanosoma equiperdum possessed PKA R-like proteins, parasites from the Venezuelan TeAp-N/D1 strain were examined using the generated anti-R(II) IgY antibodies. Western blot experiments revealed a 57-kDa polypeptide band that was distinctively recognized by these antibodies.

Nosocomial infections: Aligning strategy and action from mission to outcomes.

Outstanding health care institutions around the world lead, in effect, by setting an example and keeping hospital-acquired infections to a minimum. Behind the apparent simplicity of an indicator hides an enormous complexity. We believe that the difficulty in achieving seemingly simple benchmarks stems from the necessity to perform optimally at different levels of the organization consistently.

[Overview of the HIV/tuberculosis coinfection in Bogotá, Colombia, 2001].

The magnitude and characteristics of the co-infection of TB-AIDS was evaluated in cases reported in Bogotá, 2001. A cross sectional study was carried out using the epidemiological registry of TBC-AIDS cases from the Health Secretariat of Bogotá. Only TB cases with a positive HIV test were included in.