Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens.

Considering the objectives of "One Health" and the Sustainable development Goals "Good health and well-being" for the development of effective strategies to apply against bacterial resistance, food safety dangers, and zoonosis risks, this project explored the isolation and identification of strains from the intestinal tract of recently weaned mice; as well as the assessment of antibacterial activity against clinical and zoonotic pathogens. For molecular identification, 16S rRNA gene-specific primers were used and, via BLAST-NCBI, 16 , one and one strains were identified and registered in GenBank after the confirmation of their identity percentage and the phylogenetic analysis of the 16 strains and their association with . The 18 isolated strains showed antibacterial activity during agar diffusion tests against ATCC 15313, enteropathogenic O103, and ATCC 49943.

Effect of Starter Culture and Low Concentrations of Sodium Nitrite on Fatty Acids, Color, and Behavior during Salami Processing.

The reduction of NaNO and safety in meat products have been a concern to the meat industry for the last years. This research evaluated the changes in total fatty acids (TFAs) and myoglobin forms by adding starter culture () and 50 ppm of NaNO during salami processing. In the postripening stage, the starter culture influenced the concentration of the palmitic, oleic, vaccenic, and -linolenic TFAs, whereas the metmyoglobin concentration was lower (which could be related to the antioxidant effect of the starter culture).

modelling of simultaneous interactions of , , and .

The Jameson effect model describes suppression of microorganism growth as being dependent on non-specific competition. This model was developed for simultaneous growth in a liquid medium and microbial interactions between , and with addition of NaNO to mimic the manufacturing process of salami for 48 h at 21°C and then for 14 days at 17°C. in the presence of was inhibited by 2.

Novel extracellular proteolytic activity in Pediococcus acidilactici ATCC 8042.

Proteolytic systems are common in lactic acid bacteria, but there are few reports about proteases or peptidases in the genus Pediococcus. To evaluate the presence of these types of enzymes, Pediococcus acidilactici ATCC 8042 was cultured in MRS broth. Supernatants collected during the log phase showed proteolytic activity towards an elastin dispersion when assayed using a spectrophotometer.