In Vitro Susceptibility of Clinical and Carrier Strains of to STAFAL Phage Preparation.

The treatment of infections caused by is currently complicated by the increasing number of strains resistant to antimicrobial agents. One promising way to solve this problem is phage therapy. Due to the lack of data on the effectiveness and safety of phage preparations, STAFAL is the only registered phage preparation for the treatment of infectious diseases in the Slovak Republic and the entire European Union.

Efficiency of photodynamic inactivation Actinomyces israelii and Prevotella melaninogenica.

Background: The goal of our work was to develop a composition for antimicrobial photodynamic inactivation (aPDI) of anaerobic periodontopathogenic pathogens.

Methods: The three test groups were as follows: light plus doxycycline (L+DOX+), light plus doxycycline and hypericin (L + DOX + HYP +), and control groups. aPDI was evaluated by the number of grown colonies on a dense nutrient medium after 12, 24, and 48 hours of bacterial suspension cultivation.

Carbapenem-Resistant in COVID-19 Era-Challenges and Solutions.

The COVID-19 era brought about new medical challenges, which, together with nosocomial bacterial infections, resulted in an enormous burden for the healthcare system. One of the most alarming nosocomial threats was carbapenem-resistant (CRKP). Monitoring CRKP incidence and antimicrobial resistance globally and locally is vitally important.

High Emergence of Multidrug-Resistant Sequence Type 131 Subclade C2 among Extended-Spectrum β-Lactamase (ESBL)-Producing Isolated from the University Hospital Bratislava, Slovakia.

The expansion of sequence type 131 (ST131) extended-spectrum β-lactamase (ESBL)-producing () represents major worldwide challenges. strains originating from healthcare facilities (labeled No. 1 and No.

Moving toward Extensively Drug-Resistant: Four-Year Antimicrobial Resistance Trends of from the Largest Department of Internal Medicine in Slovakia.

imposes a great burden on medical systems worldwide. Surveillance of trends of antibiotic resistance provides a great deal of information needed for antimicrobial stewardship programmes nationwide. Clinical data from long-term, continuous surveillance on trends of antibiotic resistance of in Slovakia is missing.

Autovaccines - potent tool against chronic vulvovaginal candidiasis.

Objective: Our aim was to determine the effect of immunomodulatory therapy in women with chronic and recurrent vulvovaginal candidiasis (RVVC).

Background: We present recent highlights in the research into vaginal microbiome and consequences of chronic inflammation such as vulvovaginal candidiasis (VVC). VVC is a widespread vaginal infection primarily caused by Candida albicans.

Next-Generation Sequencing of Carbapenem-Resistant Strains Isolated from Patients Hospitalized in the University Hospital Facilities.

Carbapenem-resistant (CR) represents an urgent worldwide threat. We focused on CR in selected facilities of the University Hospital Bratislava (UHB) to investigate sequence types (STs), clonal relatedness, and antimicrobial resistance. Suspected carbapenem-nonsusceptible strains were obtained from hospitalized patients.

Susceptibility of Staphylococcus aureus strains to commercial therapeutic phage preparations.

Objectives: The aim of this study was to determine the susceptibility of Staphylococcus aureus strains to commercial phage preparations. The strains were isolated from clinical patients as well as from nasal mucosa of healthy carriers.

Background: The elevating number of antibiotic-resistant S.

Phascinating Phages.

Treatment of infections caused by bacteria has become more complex due to the increasing number of bacterial strains that are resistant to conventional antimicrobial therapy. A highly promising alternative appears to be bacteriophage (phage) therapy, in which natural predators of bacteria, bacteriophages, play a role. Although these viruses were first discovered in 1917, the development of phage therapy was impacted by the discovery of antibiotics, which spread more quickly and effectively in medical practice.

First report of nosocomial outbreak of vancomycin-resistant Enterococcus faecium infection among COVID-19 patients hospitalized in a non-intensive care unit ward in Slovakia.

Background: Enterococcus species account for most of the human enterococcal HAI and multidrug-resistant infections and have become a major threat to modern public health. We examine the rise in the number of vancomycin resistant E. faecium blood stream and urinary tract infections in a COVID-19 department during an epidemiologic outbreak investigation to detect and eliminate nosocomial clusters of the bacteria.

Complex Analysis of Vanillin and Syringic Acid as Natural Antimicrobial Agents against Biofilms.

The presence of biofilms on medical devices is a major cause of nosocomial diseases and infections. Extensive research is directed at inhibiting the formation and maturation of such biofilms. Natural plant-derived phenolic compounds have promising antimicrobial effects against drug-resistant bacteria.

Characterization of Anti-Bacterial Effect of the Two New Phages against Uropathogenic .

Urinary tract infections (UTIs) are among the events that most frequently need medical intervention. Uropathogenic are frequently their causative agents and the infections are sometimes complicated by the presence of polyresistant nosocomial strains. Phage therapy is a tool that has good prospects for the treatment of these infections.

Chronic hepatitis C virus infection in the Czech Republic and Slovakia: an analysis of patient and virus characteristics.

Objectives: The MOSAIC study gathered data on chronic hepatitis C virus (HCV) infection and its treatment in various countries worldwide. Here we summarise patient and HCV characteristics in the Czech Republic and Slovakia.

Methods: MOSAIC was an observational study that included patients with chronic HCV infection untreated at the time of enrolment.

Glucose Metabolism Changes in Patients with Chronic Hepatitis C Treated with Direct Acting Antivirals.

Background And Aims: Chronic hepatitis C is a systemic disease and type 2 diabetes mellitus (T2DM) belongs to more common extrahepatic. The aim of this study was to (i) explore the prevalence of impaired fasting glucose (IFG) and T2DM in patients with chronic hepatitis C, (ii) explore the effect of direct acting antivirals (DAA) treatment on the glycemia, and (iii) explore the factors that modulate the effect of DAA treatment on glycemia in patients with chronic hepatitis C.

Methods: We performed a longitudinal retrospective observational study focused on the patients undergoing DAA treatment of chronic hepatitis C.

Association of interleukin-10 gene promoter polymorphisms with susceptibility to acute pyelonephritis in children.

Interleukin-10 (IL-10) is a potent inhibitor of leukocyte chemotaxis, bacterial killing in phagocytes and synthesis of pro-inflammatory cytokines and chemokines, and recent studies have suggested an important role for this immunoregulatory cytokine in the pathogenesis of urinary tract infections (UTIs). Therefore, the gene encoding IL-10 (IL10) is an attractive candidate for association studies attempting to identify susceptibility genes conferring risk of UTIs. In this case-control study, we aimed to investigate the association of single nucleotide polymorphisms (SNPs) in the promoter region of IL10 with acute pyelonephritis in the Slovak population.

Phage therapy of Cronobacter-induced urinary tract infection in mice.

Background: Cronobacter spp. is an opportunistic pathogen causing rare but dangerous cases of meningitis, sepsis and urinary tract infection. Phage therapy overcomes antibiotic resistance and represents an alternative approach to standard antimicrobial treatment.

Bactofection with toll-like receptor 4 in a murine model of urinary tract infection.

The role of innate immunity in the prevention of urinary tract infection is well-documented. Toll-like receptor 4 (TLR4) is a major determinant of innate immune response. In an animal model of urinary tract infection, bactofection-mediated gene transfer of TLR4 was tested in a preventive approach.

[Selected mechanisms of resistance to antimicrobial drugs in Pseudomonas aeruginosa and their detection].

By now most clinically significant bacterial species have resistance mechanisms against almost all accessible antibiotics. One of the most significant nosocomial pathogens is Pseudomonas aeruginosa. Its antibiotic resistance directly correlates with higher morbidity, mortality and longer hospital stay.