Introduction: Sporotrichosis is an infectious fungal zoonosis associated with traumatic implantation in the skin of dimorphic fungi of the Sporothrix schenckii complex. The objective of this study was to diagnose sporotrichosis in patients in the city of Campos dos Goytacazes, and to establish correlations between positive laboratory results and dermatological and topographical aspects of the lesions and personal traits of the patients, such as sex, age and profession.
Methodology: We collected samples from 22 patients with suspect lesions, which were sent to the laboratory for direct microscopic analysis after Gram staining, followed by mycological culture by seeding the material in 4% Sabouraud dextrose agar (Himedia®) supplemented with chloramphenicol (50 mg/Lt) and cycloheximide (400 mg/Lt - Sigma-Aldrich, USA).
Ischemia is responsible for many metabolic abnormalities in the heart, causing changes in organ function. One of modifications occurring in the ischemic cell is changing from aerobic to anaerobic metabolism. This change causes the predominance of the use of carbohydrates as an energy substrate instead of lipids.
View Article and Find Full Text PDFThe aim of the study was to detect Cryptosporidium oocysts in calves feces throughout North of Rio de Janeiro State, Brazil, as well as to examine the risk factors involved in the animals infection. One hundred young calves on 13 dairy farms were analyzed for oocyst excretion. The samples were submitted to Ritchie and Ziehl-Neelsen modified tests, and the observation of the oocysts was made by using the optical microscope.
View Article and Find Full Text PDFThe aim of this study was to analyze the pleomorfism of Cryptosporidium spp. oocysts isolated from calves feces throughout the comparison of two diagnostic techniques. One hundred of cattle feces were collected and the samples were submitted to both flotation sucrose centrifugation and to Ziehl-Neelsen modified tests.
View Article and Find Full Text PDFThis study was designed to identify species of the genus Eimeria from eleven rabbit meat breeders from Campos dos Goytacazes. Fecal samples were collected from rabbits with different health conditions and consintence of feces. The following species of Eimeria were identified: E.
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