Effect of varicocele on sperm function and semen oxidative stress.

Objective: To assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity.

Patients And Methods: In all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin).

Results: The varicocele group showed fewer spermatozoa with intact DNA (grade II, P= 0.

Adolescent varicocele: improved sperm function after varicocelectomy.

Objective: To assess the effect of varicocelectomy on sperm function (DNA integrity and mitochondrial activity) and levels of lipid peroxidation in seminal plasma of adolescents.

Design: Prospective study.

Setting: Patients recruited from a local public school.

Is sperm cryopreservation an option for fertility preservation in patients with spinal cord injury-induced anejaculation?

Objective: To evaluate the effects of cryopreservation on sperm mitochondrial activity and nuclear DNA integrity in men with spinal cord injury.

Design: Prospective controlled study.

Setting: Patients in an academic research environment.

Quality and functional aspects of sperm retrieved through assisted ejaculation in men with spinal cord injury.

Objective: To assess semen quality, sperm DNA fragmentation, and mitochondrial activity in fertile men as well as in men with spinal cord injury who were collecting semen through different methods.

Design: Prospective controlled study.

Setting: Academic research environment.

Sperm nuclear DNA fragmentation and mitochondrial activity in men with varicocele.

Objective: To verify the impact of varicocele on semen quality and sperm function (DNA integrity and mitochondrial activity).

Design: Prospective study.

Setting: Patients in an academic research environment.