A Semi-Selective Medium to Evaluate Over-Summering Survival of sp. in Florida Strawberry Fields.

Devastating disease outbreaks with leaf spot symptoms and fruit rot caused by a fungus identified as a cryptic species of have generated concern in the Florida strawberry industry. Some species are saprobes in soil and plant debris, but the ability of the new sp. to survive in strawberry debris was unknown.

Baseline Sensitivity of Isolates from Strawberry to Isofetamid Compared to other SDHIs.

Succinate dehydrogenase inhibitors (SDHIs) are the fungicides most commonly used to control Botrytis fruit rot on commercial strawberry in Florida. The medium-to-high risk of selection of resistance in the causal agent is a threat to the efficacy of this fungicide group. In this study, we characterized the sensitivity of to the SDHI isofetamid, evaluated the SdhB gene mutation associated with resistance, and monitored resistance frequencies to five SDHI fungicides for two consecutive seasons.

Mutations in the Membrane-Anchored SdhC Subunit Affect Fitness and Sensitivity to Succinate Dehydrogenase Inhibitors in Populations from Multiple Hosts.

Succinate dehydrogenase inhibitors (SDHIs) are an essential group of fungicides for managing gray mold, caused by , in numerous crops. Resistance to boscalid, an early-generation SDHI, is widespread worldwide and was linked to mutations in the iron-sulfur protein encoding the SdhB subunit of the SDH complex. Herein, we report on four simultaneous dependent mutations at codons 85 (G85A), 93 (I93V), 158 (M158V), and 168 (V168I) of the membrane-anchored SdhC subunit of .

The Importance of Selecting Appropriate Rotation and Tank-Mix Partners for Novel SDHIs to Enhance Botrytis Fruit Rot Control in Strawberry.

Botrytis fruit rot (BFR), caused by the necrotrophic fungus Botrytis cinerea, is the most important disease of strawberry and is mainly controlled by applications of fungicides from multiple chemical groups. To develop more effective and sustainable BFR management programs, field trials were conducted to evaluate the efficacy of fluopyram and penthiopyrad, two newly registered succinate dehydrogenase inhibitors (SDHIs), rotated or tank mixed with the multisites thiram and captan or the single-sites fludioxonil and fenhexamid. The treatments were applied at two different strawberry fields during the 2013-14 and 2014-15 seasons.

Prevalence of Botrytis Cryptic Species in Strawberry Nursery Transplants and Strawberry and Blueberry Commercial Fields in the Eastern United States.

Botrytis isolates from strawberry transplants originating from Canada and the northern United States as well as isolates collected from strawberry and blueberry commercial fields in the southeastern United States were investigated for the frequency of Botrytis cinerea, other cryptic Botrytis spp. reported recently, and the transposable elements (TE) using six genetic markers. B.

Sources of Primary Inoculum of Botrytis cinerea and Their Impact on Fungicide Resistance Development in Commercial Strawberry Fields.

Strawberry transplants produced in nurseries across Canada, northern United States, and California are shipped annually to other strawberry-growing regions, including Florida. Botrytis cinerea, the causal agent of gray mold, causes latent infections on transplants which are suggested as a potential source of primary inoculum in strawberry fields. In this study, we investigated the survival of B.