Publications by authors named "Adrian Carcamo"
Functional genomics and chemical screens can identify and characterize novel cellular factors regulating signaling networks and chemical tools to modulate their function for the treatment of disease. Screening methods have relied primarily on immortalized and/or transformed cancer cell lines, which can limit the generalization of results to more physiologically relevant systems. Most have also relied on immunofluorescence, or on stably expressed recombinant fluorescent proteins, to detect specific protein markers using high-content imaging readouts.
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- Single-cell measurement techniques have enhanced our understanding of gene expression variability in healthy and diseased states, revealing significant differences in individual cell transcription and responses to changes.
- The new method called hcHCR integrates hybridization chain reaction for RNA visualization with high-content imaging to measure changes in gene expression in human immune cells effectively.
- The system allows for easy detection of gene expression changes in thousands of cells simultaneously, enables multiplexing to assess multiple transcripts, and is adaptable for use in patient-derived cells for research and testing.