A novel HPLC method for the concurrent analysis and quantitation of seven water-soluble vitamins in biological fluids (plasma and urine): a validation study and application.

An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B(1), B(2), B(5), B(6), B(9), B(12)) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol.

Chronic alcohol feeding and its influence on c-Fos and heat shock protein-70 gene expression in different brain regions of male and female rats.

Excessive ingestion of the macronutrient, alcohol, causes devastating complications in the brain leading to atrophy and impaired cognitive function with corresponding increases in morbidity and mortality and, consequently, reduced quality of life measures. The pathogenic mechanisms are unknown, but various studies have shown that the immediate early genes and heat shock (ie, stress or chaperone) proteins are increased in alcohol-exposed tissue. However, many of these studies have been performed in vitro or have failed to consider either the nutritional elements in the experimental design by appropriate use of pair-feeding or whether there are regional and/or gender differences.